Microarray analyzer based on wide field fluorescent microscopy with laser illumination and a device for speckle suppression

Lysov, Yuri; Barsky, V. E.; Urasov, D. A.; Urasov, R. A.; Cherepanov, A. V.; Mamaev, D. D.; Yegorov, Y. Y.; Чудинов, А. В.; Суржиков, С. А.; Rubina, A. Yu.; Smoldovskaya, Olga; Заседателев, А. С.

doi:10.1364/boe.8.004798

Cited by 32 publications

(14 citation statements)

References 17 publications

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“…The registration of fluorescence signals was performed using a two-wavelength microarray analyser (EIMB RAS) based on the concept of digital wide-field fluorescence microscopy [ 17 ]. The measuring range of the method is 0.25-100 IU/ml for sIgE and 100-2500 ng/ml for sIgG4.…”

Section: Methodsmentioning

confidence: 99%

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

et al. 2018

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The immunological profiles of human specific IgE (sIgE) and specific IgG4 (sIgG4) vary by genetic predisposition, living conditions in different geographical locations and patient’s age. The aim of our study was to analyze sIgE and sIgG4 patterns and their age-dependent changes in patients from the Moscow region. For identifying sIgE and sIgG4 profiles the blood samples from 513 patients aged 6 months to 17 years who were showing symptoms of allergic diseases were analyzed using microarrays containing 31 allergens. The highest sIgE prevalence was observed for birch pollen (32%) among pollen allergens, cat dander (24%) among indoor allergens, and egg whites (21%) among food allergens. The most common sIgG4 response was developed toward egg whites (80% of patients). Age-related elevation was identified for patients with increased sIgE to pollen allergens and indoor allergens (cat or dog dander and house dust mites). For each allergen, the proportion of cases with significant levels of sIgG4 appeared to increase with patients’ age. The data on allergen-specific sIgE and sIgG4 prevalence show both general trends and some local special aspects that are indicative for the Moscow region. This information should be useful in terms of epidemiology of allergic diseases.

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Section: Methodsmentioning

confidence: 99%

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

et al. 2018

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“…After the analysis, fluorescent signals from the gel pads with immobilized allergens are detected using a microarray analyzer with laser illumination and a device for speckle suppression [ 15 ]. Fluorescent signals are calculated by referencing piecewise linear calibration curves constructed on the basis of the signals from the gel pads with immobilized IgE and IgG4 to obtain the sIgE and sIgG4 concentrations in IU/ml and ng/ml, respectively.…”

Section: Methodsmentioning

confidence: 99%

Allergen-specific IgE and IgG4 patterns among patients with different allergic diseases

Smoldovskaya

Feyzkhanova

Voloshin

et al. 2018

World Allergy Organization Journal

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BackgroundIn addition to allergen-specific IgE (sIgE), allergen-specific IgG4 (sIgG4) antibodies are also involved in the immune response resulting from an allergen exposure. The aim of our study was to analyze sIgE and sIgG4 patterns in the most common allergic disorders: bronchial asthma, upper airway disorders and atopic dermatitis.MethodsIn this study a screening analysis of blood serum samples from 673 patients aged from 6 months to 17 years with different allergic entities was performed on microarrays. sIgE and sIgG4 levels to the most common allergens were estimated.ResultssIgE response to most pollen allergens is more strongly associated with respiratory diseases than with atopic dermatitis, while sIgE responses to cat and dog dander are more strongly associated with bronchial asthma than with atopic dermatitis and upper airway disorders such as rhinosinusitis and allergic rhinitis. A lower prevalence of sIgG4 to pollen allergens in cases of atopic dermatitis is observed compared with that in cases of asthma and upper airway disorders. Analyzing all the allergic disorders, one can see that sIgG4 response to inhalant allergens is strongly associated with sensitization to the corresponding allergen.ConclusionAllergen-specific IgE and IgG4 patterns that are relevant to concrete allergic diseases differ by sIgE and sIgG4 prevalences to defined allergens.Electronic supplementary materialThe online version of this article (10.1186/s40413-018-0220-5) contains supplementary material, which is available to authorized users.

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“…Several generations of universal fluorescence analyzers have been designed. The most recent one can be used to measure the signal intensity of microarray elements at wavelengths ranging from 380 to 850 nm and perform qualitative and quantitative analyses with an accuracy of ± 5% [20].…”

Section: The Key Aspects Of a Microarray Analysismentioning

confidence: 99%

The EIMB Hydrogel Microarray Technology: Thirty Years Later

Gryadunov¹,

Shaskolskiy²,

Наседкина³

et al. 2018

Acta Naturae

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Biological microarrays (biochips) are analytical tools that can be used to implement complex integrative genomic and proteomic approaches to the solution of problems of personalized medicine (e.g., patient examination in order to reveal the disease long before the manifestation of clinical symptoms, assess the severity of pathological or infectious processes, and choose a rational treatment). The efficiency of biochips is predicated on their ability to perform multiple parallel specific reactions and to allow one to study the interactions of biopolymer molecules, such as DNA, proteins, glycans, etc. One of the pioneers of microarray technology was the Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences (EIMB), with its suggestion to immobilize molecular probes in the three-dimensional structure of a hydrophilic gel. Since the first experiments on sequencing by hybridization on oligonucleotide microarrays conducted some 30 years ago, the hydrogel microarrays designed at the EIMB have come a long and successful way from basic research to clinical laboratory diagnostics. This review discusses the key aspects of hydrogel microarray technology and a number of state-ofthe-art approaches for a multiplex analysis of DNA and the protein biomarkers of socially significant diseases, including the molecular genetic, immunological, and epidemiological aspects of pathogenesis. KEYWORDS hydrogel microarrays, nucleic acid hybridization, multiplex immunochemical assay, antimicrobial drug resistance, genotyping, tumor markers. ABBREVIATIONS NAs -nucleic acids; NGS -next-generation sequencing; FDA -Food and Drug Administration (USA); MTB -Mycobacterium tuberculosis, causative agent of tuberculosis; NTM -non-tuberculous mycobacteria, causative agents of mycobacteriosis; HCV -hepatitis C virus; RMP -rifampin; INH -isoniazid; EMBethambutol; MDR -multidrug resistance; XDR -extensive drug resistance; RTIs -reproductive tract infections; AMD -antimicrobial drugs; CRC -colorectal carcinoma; CEA -carcinoembryonic antigen; CA -carbohydrate antigen. REVIEWS VOL. 10 № 4 (39) 2018 | ACTA NATURAE | 5

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Microarray analyzer based on wide field fluorescent microscopy with laser illumination and a device for speckle suppression

Cited by 32 publications

References 17 publications

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

Allergen-specific IgE and IgG4 patterns among patients with different allergic diseases

The EIMB Hydrogel Microarray Technology: Thirty Years Later

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