Microarray evaluation of specific IgE to allergen components in elite athletes

Abstract

The ImmunoCAP ISAC represents a useful additional tool for diagnosis and management of poly-sensitized athletes.

Allergen extract vs. component sensitization and airway inflammation, responsiveness and new‐onset respiratory disease

“…Further, high concordance has been shown between the use of timothy extract and allergen components measured with multiplex technique [12,13]. The PPAs for timothy and cat were lower than those presented by Bonini et al [12] and Melioli et al [13]. The PPAs for timothy and cat were lower than those presented by Bonini et al [12] and Melioli et al [13].…”

“…To evaluate the agreement between allergen extract and component IgE testing, calculations were made of the positive per cent agreement (PPA), that is the percentage of subjects positive to allergen components (>0.3 ISU) out of all subjects who were positive using the extractbased method at an either cut-off of >0.35 kUA/L or an arbitrary chosen [12,13] higher cut-off of >1 kUA/L, and of the negative per cent agreement (NPA), that is the percentage of subjects who were negative to allergen components (<0.3 ISU) out of all subjects who were negative to the extract. FeNO, S-ECP values and IgE antibody concentrations (both methods of assessment) were log-transformed before statistical analysis.…”

“…There is generally a high concordance between single and multiplex measurements of IgE to allergen components [11], as well as between measurements using allergen extract and the corresponding components [15][16][17][18][19][20]. However, differences have been reported for some allergens [12,13], with a subgroup of subjects being sensitized according to extract-based measurements, but not according to measurements using components. The clinical importance of this discordance has not been studied in relation to present airway inflammation and responsiveness or in relation to later development of allergic airway disease.…”

“…Several child cohort studies [8][9][10] have examined sensitization using both extract-based tests and allergen components, although none of these studies included a direct comparison. Only a few epidemiological studies [11][12][13] have compared IgE testing towards allergen extracts with testing towards allergen components, and all these studies have had a cross-sectional design [14]. There is generally a high concordance between single and multiplex measurements of IgE to allergen components [11], as well as between measurements using allergen extract and the corresponding components [15][16][17][18][19][20].…”

Allergen-Based Diagnostic: Novel and Old Methodologies with New Approaches

“…First, the sIgE from serum samples interacts with the recombinant allergen previously adsorbed to the solidphase; then, a secondary anti-human IgE antibody labeled with fluorochrome recognizes sIgE-recombinant allergen complex (Figure 7). Fluorescence is measured using a biochip; and results are scanned and analyzed in specialized software, reporting results in arbitrary units named ISAC Standardized Units (ISU) (Figure 8) [32]. Table 2.…”