Microbacterium oryzae sp. nov., an actinobacterium isolated from rice field soil

Abstract

A novel aerobic soil actinobacterium (strain MB10 T ) belonging to the genus Microbacterium was isolated from rice field soil samples collected from Jagatpur, Orissa, India. Cells were Gram-stain positive, short rod-shaped and motile. The strain was oxidase-negative and catalase-positive. Heterotrophic growth was observed at pH 5.0-11.0 and at 16-37 6C; optimum growth was observed at 28 6C and pH 7.0-9.0. The DNA G+C content was 71.6 mol%. Predominant cellular fatty acids of strain MB10 T were iso-C 14 : 0 , anteiso-C 15 : 0 , C 16 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . Cell wall sugars were galactose, glucose and rhamnose. The major isoprenoid quinones were MK-9 (10 %), MK-10 (43 %) and MK-11 (36 %). The peptidoglycan represents the peptidoglycan type B2b. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid and unknown glycolipids. 16S rRNA gene sequence identity revealed the strain MB10 T clustered within the radiation of the genus Microbacterium and showed 99.2 % similarity with Microbacterium barkeri DSM 20145 T . However, DNA-DNA similarity study was 37.0 % with Microbacterium barkeri DSM 20145 T , the nearest phylogenetic relative. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence analysis and DNA-DNA reassociation studies, it is proposed that strain MB10 T represents a novel species of the genus Microbacterium, for which the name Microbacterium oryzae sp. nov. is proposed; the type strain is MB10 T (5JCM 16837 T 5DSM 23396 T ).

Taxonomic study of the genus Tepidiphilus: transfer of Petrobacter succinatimandens to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov., emended description of the genus Tepidiphilus and description of Tepidiphilus thermophilus sp. nov., isolated from a terrestrial hot spring

“…Cell morphology was examined by using transmission electron microscopy (model MORGAGNI 268D; FEI) as described by Kumari et al (2013). Gram staining was carried out using a commercial kit (Becton Dickinson) according to the manufacturer’s instructions.…”

“…All strains were at the stationary phase of growth at the time of fatty acid analysis. Analysis was performed by the method described by Kumari et al (2013). Fatty acid methyl ester mixtures were separated by using the Sherlock Microbial identification system (MIDI, Microbial ID) with an Agilent model 6890N gas chromatograph.…”

Microbacterium nanhaiense sp. nov., an actinobacterium isolated from sea sediment

“…At the time of writing, the genus Microbacterium comprised 88 recognized species with validly published names (http://www.bacterio.net/microbacterium.html). Strains representing the genus Microbacterium are ubiquitously distributed in various environments including soil, insects, marine environments, human clinical specimens, plants, dairy products and sewage sludge compost (Takeuchi & Hatano, 1998b; Brennan et al , 2001; Rivas et al , 2004; Lee et al , 2006; Park et al , 2006; Shivaji et al , 2007; Bakir et al , 2008; Vaz-Moreira et al , 2008; Anand et al , 2012; Kämpfer et al , 2012; Kumari et al , 2013; Yu et al , 2013). They have also been found in the phyllospheres of sugar beet and spring wheat, and as endophytes in cotton (Thompson et al , 1993; Legard et al , 1994; McInroy & Kloepper, 1995).…”

Marinomonas fungiae sp. nov., isolated from the coral Fungia echinata from the Andaman Sea

“…Cellular fatty acids were analysed from cells grown to stationary phase on MA at 28 °C for 3 days. The remaining procedures for fatty acids analysis were as described by Kumari et al (2013). Analysis of the fatty acid profile of strain AN44 T revealed eight major fatty acids (accounting for 97.3 % of the total fatty acids): C 18 : 1 ω7 c (49.1 %), C 16 : 1 ω7 c /C 16 : 1 ω6 c (19.9 %), C 16 : 0 (11.1 %), C 10 : 0 3-OH (6.7 %), C 12 : 0 (3.7 %), C 10 : 0 (3.5 %), C 14 : 0 (1.8 %) and C 18 : 0 (1.2 %).…”