Microbial adhesion and ultrastructure from the single-molecule to the single-cell levels by Atomic Force Microscopy

Beaussart, Audrey; El-Kirat-Chatel, Sofiane

doi:10.1016/j.tcsw.2019.100031

Cited by 24 publications

(17 citation statements)

References 122 publications

(177 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity of these antimicrobial peptides allows the selective disruption of cell membranes, which leads to membrane permeabilization, depolarization, dissipation of electrochemical gradients, and eventual cell death [54]. Microbial cell walls are covered by polyanionic molecules such as lipoteichoic acids in Gram-positive bacteria, while in Gram-negative bacteria the peptidoglycan layer is thinner and is surrounded by two phospholipids membranes, with the outer membrane being topped by lipopolysaccharides [55]. Therefore, the effectiveness of the porcine liver hydrolysates could be due to the cationic and hydrophobic properties of the peptides, which allow the reaction with the internal cytoplasmic anionic membranes of the microbes, causing their breakdown [10].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Evaluation of the Antioxidant and Antimicrobial Activities of Porcine Liver Protein Hydrolysates Obtained Using Alcalase, Bromelain, and Papain

et al. 2020

View full text Add to dashboard Cite

In order to make the by-products generated from the porcine industry more valuable, pig livers were used in this trial to obtain protein hydrolysates. Three proteases (alcalase, bromelain, and papain) were utilized for enzymatic hydrolysis with two different durations, 4 and 8 hours. Ultrafiltration process was used for the recovery of the extracts, employing three different membrane pore sizes (30, 10, and 5 kDa). The porcine livers contained considerable amounts of protein (19.0%), considering they are almost composed of water (74.1%). The antioxidant activity of the obtained hydrolysates was investigated using four antioxidant methods (2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2-2′-Azino-di-[3-ethylbenzthiazoline sulfonate] (ABTS) radical scavenging activity, ferric reducing antioxidant power assay (FRAP), and oxygen radical absorbance capacity assay (ORAC)). Antibacterial properties were also measured against Gram-negative and Gram-positive bacteria. Results indicated that the three studied factors (type of enzyme, membrane pore size, and time) significantly affected the parameters evaluated. Hydrolysates obtained at 8 hours with alcalase had the best antioxidant properties. The 30 kDa alcalase extracts exhibited the highest DPPH (562 µg Trolox/g), FRAP (82.9 µmol Fe2+/100 g), and ORAC (53.2 mg Trolox/g) activities, while for ABTS the 10 kDa alcalase showed the higher values (1068 mg ascorbic acid/100 g). Concerning the antibacterial activity, 30 kDa hydrolysates obtained with bromelain for 4 hours exhibited the highest antimicrobial capacity, providing an inhibition of 91.7%.

show abstract

Section: Antimicrobial Activitymentioning

confidence: 99%

Evaluation of the Antioxidant and Antimicrobial Activities of Porcine Liver Protein Hydrolysates Obtained Using Alcalase, Bromelain, and Papain

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The bacterial cell wall is composed of peptidoglycan, which is structured around a poly-( N -acetylglucosamine- N -acetylmuramic acid) backbone [ 53 ]. Gram-positive bacteria differ from Gram-negative bacteria because of their thick peptidoglycan layer and the absence of lipopolysaccharide (LPS) [ 54 ]. Stronger inhibition of viable Gram-negative bacteria has also been reported in some studies [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel Strategy for Creating an Antibacterial Surface Using a Highly Efficient Electrospray-Based Method for Silica Deposition

Levana

Hong

Kim

et al. 2022

IJMS

View full text Add to dashboard Cite

Adhesion of bacteria on biomedical implant surfaces is a prerequisite for biofilm formation, which may increase the chances of infection and chronic inflammation. In this study, we employed a novel electrospray-based technique to develop an antibacterial surface by efficiently depositing silica homogeneously onto polyethylene terephthalate (PET) film to achieve hydrophobic and anti-adhesive properties. We evaluated its potential application in inhibiting bacterial adhesion using both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria. These silica-deposited PET surfaces could provide hydrophobic surfaces with a water contact angle greater than 120° as well as increased surface roughness (root mean square roughness value of 82.50 ± 16.22 nm and average roughness value of 65.15 ± 15.26 nm) that could significantly reduce bacterial adhesion by approximately 66.30% and 64.09% for E. coli and S. aureus, respectively, compared with those on plain PET surfaces. Furthermore, we observed that silica-deposited PET surfaces showed no detrimental effects on cell viability in human dermal fibroblasts, as confirmed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide and live/dead assays. Taken together, such approaches that are easy to synthesize, cost effective, and efficient, and could provide innovative strategies for preventing bacterial adhesion on biomedical implant surfaces in the clinical setting.

show abstract

“…Complementary to biochemical assays and omics methods, the strength of AFM lies in its ability to analyze and nanomanipulate single cells with high spatial-temporal resolution (reviewed in (Li et al, 2019). Single-cell analysis is crucial for understanding the adhesion that drives the first-steps of hostpathogen interactions (Beaussart and El-Kirat-Chatel, 2019). Many molecular methods have been used to study host-pathogen surface interactions, but only AFM can localize and quantify those interactions at the nm and pN scale, respectively.…”

Section: Quantifying Host-pathogen Interactionsmentioning

confidence: 99%

AFM-Based Correlative Microscopy Illuminates Human Pathogens

Bhat

Price

Dahms

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Microbes have an arsenal of virulence factors that contribute to their pathogenicity. A number of challenges remain to fully understand disease transmission, fitness landscape, antimicrobial resistance and host heterogeneity. A variety of tools have been used to address diverse aspects of pathogenicity, from molecular host-pathogen interactions to the mechanisms of disease acquisition and transmission. Current gaps in our knowledge include a more direct understanding of host-pathogen interactions, including signaling at interfaces, and direct phenotypic confirmation of pathogenicity. Correlative microscopy has been gaining traction to address the many challenges currently faced in biomedicine, in particular the combination of optical and atomic force microscopy (AFM). AFM, generates high-resolution surface topographical images, and quantifies mechanical properties at the pN scale under physiologically relevant conditions. When combined with optical microscopy, AFM probes pathogen surfaces and their physical and molecular interaction with host cells, while the various modes of optical microscopy view internal cellular responses of the pathogen and host. Here we review the most recent advances in our understanding of pathogens, recent applications of AFM to the field, how correlative AFM-optical microspectroscopy and microscopy have been used to illuminate pathogenicity and how these methods can reach their full potential for studying host-pathogen interactions.

show abstract

Microbial adhesion and ultrastructure from the single-molecule to the single-cell levels by Atomic Force Microscopy

Cited by 24 publications

References 122 publications

Evaluation of the Antioxidant and Antimicrobial Activities of Porcine Liver Protein Hydrolysates Obtained Using Alcalase, Bromelain, and Papain

Evaluation of the Antioxidant and Antimicrobial Activities of Porcine Liver Protein Hydrolysates Obtained Using Alcalase, Bromelain, and Papain

A Novel Strategy for Creating an Antibacterial Surface Using a Highly Efficient Electrospray-Based Method for Silica Deposition

AFM-Based Correlative Microscopy Illuminates Human Pathogens

Contact Info

Product

Resources

About