Microbial bioconversion of thermally depolymerized polypropylene by Yarrowia lipolytica for fatty acid production

Mihreteab, Merhawi; Stubblefield, Bryan A.; Gilbert, Eric S

doi:10.1007/s00253-019-09999-2

Cited by 46 publications

(18 citation statements)

References 32 publications

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“…This result might be due to the low gene expression level or even the absence of certain PP-degrading enzymes in P. aeruginosa , meaning that defective enzyme functions could then reduce the PP biodegradation rate. It has been reported that Yarrowia lipolytica 78-003, a bacterial strain, can convert PP to fatty acids and then to the energy source PHA [ 40 ]. Therefore, insufficient raw carbon fragments resulting from the extremely slow PP biodegradation rate and/or the defective enzyme function for converting PP to the corresponding energy source PHA by P. aeruginosa might have further limited the increase of the bacterial population ( Figure 6 D).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Biodegradation Efficiency of Four Various Types of Plastics by Pseudomonas aeruginosa Isolated from the Gut Extract of Superworms

et al. 2020

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Plastic waste worldwide is becoming a serious pollution problem for the planet. Various physical and chemical methods have been tested in attempts to remove plastic dumps. However, these have usually resulted in secondary pollution issues. Recently, the biodegradation of plastic by fungal and bacterial strains has been spotlighted as a promising solution to remove plastic wastes without generating secondary pollution. We have previously reported that a Pseudomonas aeruginosa strain isolated from the gut of a superworm is capable of biodegrading polystyrene (PS) and polyphenylene sulfide (PPS). Herein, we demonstrate the extraordinary biodegradative power of P. aeruginosa in efficiently depolymerizing four different types of plastics: PS, PPS, polyethylene (PE) and polypropylene (PP). We further compared biodegradation rates for these four plastic types and found that PE was biodegraded fastest, whereas the biodegradation of PP was the slowest. Moreover, the growth rates of P. aeruginosa were not always proportional to biodegradation rates, suggesting that the rate of bacterial growth could be influenced by the composition and properties of intermediate molecules produced during plastic biodegradation, and these may supply useful cellular precursors and energy. In conclusion, an initial screening system to select the most suitable bacterial strain to biodegrade certain types of plastic is particularly important and may be necessary to solve plastic waste problems both presently and in the future.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Biodegradation Efficiency of Four Various Types of Plastics by Pseudomonas aeruginosa Isolated from the Gut Extract of Superworms

et al. 2020

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“…In 2019, Mihreteab et al reported that strain Yarrowia lipolytica 78-003 was able to convert such depolymerization products to value-added fatty acids when mixed with biosurfactants and trace nutrients. During a period of 312 h, Y. lipolytica 78-003 assimilated more than 80% of the substrate and produced up to 492 mg L −1 lipids mainly composed of C 16 -C 18 unsaturated fatty acids (Mihreteab et al, 2019). Johnston et al (2019) found that R. eutropha H16 could utilize oxidized PP fragments as an additional carbon source to produce PHA in TSB medium.…”

Section: From Aromatic Hydrocarbons To Succinic Acids and Phamentioning

confidence: 99%

Microbial Degradation and Valorization of Plastic Wastes

2020

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“…Despite the lack of well-studied depolymerizing enzymes, polystyrene, polypropylene, and polyethylene can be non-enzymatically treated, e.g., via pyrolysis. The resulting long-chain hydrocarbons can then be readily metabolized by microbes such as Yarrowia lipolytica (Mihreteab et al, 2019). The partial or complete plastic degradation pathways from existing microorganisms could potentially be incorporated into recombinant microbial hosts to not only break down polymers but also convert the degradation products into fuels, valuable chemicals, or biodegradable plastics.…”

Section: Plastic Waste Degradation and Conversion To Biofuelsmentioning

confidence: 99%

Biofuels for a sustainable future

Liu

Cruz-Morales

Zargar

et al. 2021

Cell

246

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Rapid increases of energy consumption and human dependency on fossil fuels have led to the accumulation of greenhouse gases and consequently, climate change. As such, major efforts have been taken to develop, test, and adopt clean renewable fuel alternatives. Production of bioethanol and biodiesel from crops is well developed, while other feedstock resources and processes have also shown high potential to provide efficient and cost-effective alternatives, such as landfill and plastic waste conversion, algal photosynthesis, as well as electrochemical carbon fixation. In addition, the downstream microbial fermentation can be further engineered to not only increase the product yield but also expand the chemical space of biofuels through the rational design and fine-tuning of biosynthetic pathways toward the realization of ''designer fuels'' and diverse future applications.

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Microbial bioconversion of thermally depolymerized polypropylene by Yarrowia lipolytica for fatty acid production

Cited by 46 publications

References 32 publications

Evaluation of the Biodegradation Efficiency of Four Various Types of Plastics by Pseudomonas aeruginosa Isolated from the Gut Extract of Superworms

Evaluation of the Biodegradation Efficiency of Four Various Types of Plastics by Pseudomonas aeruginosa Isolated from the Gut Extract of Superworms

Microbial Degradation and Valorization of Plastic Wastes

Biofuels for a sustainable future

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