This study highlights the development of a lab-scale, indigenously designed; Packed-Bed Bio lm Reactors (PBBR) packed with brick pieces. The developed bio lm in the reactor was used for the decolourisation and biodegradation of the textile industry e uent. The PBBR was continuously operated for 264 days, during which 301 cycles of batch and continuous treatment were operated. In batch mode under optimised conditions, more than 99% dye decolourisation and ≥92% COD reduction were achieved in 6 h of contact time upon supplementation of e uent with 0.25 g L -1 glucose, 0.25 g L -1 urea, and 0.1 g L -1 phosphates. A decolourisation rate of 133.94 ADMI units h -1 was achieved in the process. PBBR, when operated in continuous mode, showed ≥95% and ≥92% reduction in ADMI and COD values. Subsequent aeration and passage through charcoal reactor assisted in achieving ≥96 % reduction in COD and ADMI values. An overall increase of 81% in dye-laden e uent decolourisation rate, from 62 mg L -1 h -1 to 262 mg L -1 h -1 , was observed upon increasing the ow rate from 18 mL h -1 to 210 mL h -1 . Dye biodegradation was determined by UV-Vis and FTIR spectroscopy and toxicity study. SEM analysis showed the morphology of the attached-growth bio lm.
HighlightsPacked-Bed Bio lm Reactor with an indigenous consortium was developed for textile e uent treatment.The use of developed consortia and optimisation resulted in more than 92% of COD and 99% of dye removal.FTIR analysis was conducted for the detection and quanti cation of the dye metabolites. SEM analysis was used to examine the morphology of the attached-growth bio lm.The developed reactor was successfully run for 264 d with 301 cycles.