Microbial Co-occurrence Network and Fermentation Information of Natural Woody-Plant Silage Prepared With Grass and Crop By-Product in Southern Africa

Du, Zhumei; Yamasaki, Seishi; Oya, Tetsuji; Nguluve, Damiao; Euridse, Denise; Tinga, Benedito; Macome, Felicidade Margarida; Cai, Yimin

doi:10.3389/fmicb.2022.756209

Cited by 11 publications

(20 citation statements)

References 42 publications

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“…Ensiling without the use of an inoculant or starter is a spontaneous fermentative process in which fermentation mainly depends on the composition of epiphytic microorganisms and the natural occurrence of epiphytic LAB ( Guo et al, 2018 ). Many studies have shown that by using the PacBio SMRT sequencing, microorganism composition and its dynamics in the ensiling process can be precisely revealed at the species level because it can generate long sequence reads, which provides valuable biological information regarding the complete bacterial community in the ensiling system ( Bai et al, 2021 ; Du et al, 2022a , b ). The bacteria in all samples of fresh and silage ryegrass in this study were sequenced by SMRT sequencing technology to accurately assess the microbial community and diversity ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, in this study, compared with the BK group, the relative abundance of Enterococcus in CK and AK silages was higher, so the lactic acid content of CK and AK was lower than that of BK. Since Enterococcus are not acid-resistant genera and the pH value of BK was lower, there were few Enterococcus species in BK, and these were mostly Lactiplantibacillus plantarum , which were found to be more acid tolerant ( Du et al, 2022b ). In addition, more butyric acid and NH 3 -N accumulation in BK may have been due to higher protein content in the second ryegrass crop resulting in a higher protein degradation rate.…”

Section: Discussionmentioning

confidence: 99%

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Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage

et al. 2022

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In this study, we analyzed the fermentation quality, microbial community, and metabolome characteristics of ryegrass silage from different harvests (first harvest-AK, second harvest-BK, and third harvest-CK) and analyzed the correlation between fermentative bacteria and metabolites. The bacterial community and metabolomic characteristics were analyzed by single-molecule real-time (SMRT) sequencing and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS), respectively. After 60 days of ensiling, the pH of BK was significantly lower than those of AK and CK, and its lactic acid content was significantly higher than those of AK and CK. Lactiplantibacillus and Enterococcus genera dominate the microbiota of silage obtained from ryegrass harvested at three different harvests. In addition, the BK group had the highest abundance of Lactiplantibacillus plantarum (58.66%), and the CK group had the highest abundance of Enterococcus faecalis (42.88%). The most annotated metabolites among the differential metabolites of different harvests were peptides, and eight amino acids were dominant in the composition of the identified peptides. In the ryegrass silage, arginine, alanine, aspartate, and glutamate biosynthesis had the highest enrichment ratio in the metabolic pathway of KEGG pathway enrichment analysis. Valyl-isoleucine and glutamylvaline were positively correlated with Lactiplantibacillus plantarum. D-Pipecolic acid and L-glutamic acid were positively correlated with Levilactobacillus brevis. L-phenylalanyl-L-proline, 3,4,5-trihydroxy-6-(2-methoxybenzoyloxy) oxane-2-carboxylic acid, and shikimic acid were negatively correlated with Levilactobacillus brevis. In conclusion, this study explains the effects of different harvest frequencies on the fermentation quality, microbial community, and metabolites of ryegrass, and improves our understanding of the ensiling mechanisms associated with different ryegrass harvesting frequencies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage

et al. 2022

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“…( Du et al, 2020 ). The utilization of fermentation substrates by LAB is limited ( Bai et al, 2020 ), and excess fermentation substrate may allow some unfavorable microorganisms with strong tolerance (such as acid tolerance and facultative anaerobic activity) to multiply or remain latent ( Wang et al, 2019a ; Yan et al, 2019 ; Du et al, 2022 ), which also partially explains the higher microbial diversity in NA3 and NA9 ( Figure 2 ). In this study, the treatment that was neither inoculated with LAB nor supplemented with corn flour (NA) had a high pH and low LA content.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, these complex microbial structures and interactions have been well characterized thanks to the single-molecule real-time (SMRT) technology that can cover full-length DNA fragments integrated on the PacBio platform. SMRT technology is also widely used to reveal the microbial environment of silage such as whole-plant corn, alfalfa, Napier grass, and sugarcane due to its high throughput, low error rate, and ability to trace microorganisms to the species or even strain level ( Cai et al, 2020 ; Bai J. et al, 2021 ; Xu D. et al, 2021 ; Du et al, 2022 ). To the best of our knowledge, so far, no studies revealing complete microbial information containing whole-plant paper mulberry silage have been reported.…”

Section: Introductionmentioning

confidence: 99%

Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage

Sun

Huang

et al. 2022

Front. Microbiol.

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The purpose of this study was to investigate the effects of inoculation with two lactic acid bacteria (LAB) strains (Lacticaseibacillus rhamnosus and Lentilactobacillus buchneri) and the addition of four corn flour proportions (0, 3, 6, and 9%) in different treatments, on the composition and function of the bacterial community in whole-plant paper mulberry silage. The different treatments promoted Lactiplantibacillus, Lentilactobacillus, and Lacticaseibacillus growth, but the microbial species responsible for fermentation differed among the treatments. High species diversity and various Gram-negative bacteria, such as Flavitalea sp., Pantoea agglomerans, Acinetobacter pittii, Turicibacter sanguinis, and Ralstonia pickettii, were found in the uninoculated LAB treatments. A beneficial bacterium, Lactobacillus johnsonii, was discovered for the first time in whole-plant paper mulberry silage. LAB inoculation simplifies the microbial community structure, and beneficial Lactobacillus as a key species aggregates in the inoculated treatment group. However, L. rhamnosus inoculation alone may have limited bacteriostatic activity against in whole-plant paper mulberry silage. Compared with silage lacking corn flour, amino sugar and nucleotide sugar metabolism, galactose metabolism, the phosphotransferase system and the pentose phosphate pathway metabolic activity were increased in corn flour-containing silage. Whole-plant paper mulberry can be used as a high-quality silage to provide high-quality feed resources for sustainable ruminant livestock production. Moreover, additive use is necessary for preparing paper mulberry silage.

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“…Silage has the advantages of a strong sour taste and a moist and soft texture, which can improve its palatability to ruminants [13][14][15]. In addition, silage that can be stored can ensure a balanced supply of feed throughout the year and solve the problem of seasonal feed shortages in tropical regions.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Lactic Acid Bacteria Isolated from Banana and Its Application in Silage Fermentation of Defective Banana

et al. 2022

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To effectively utilize banana by-products, we prepared silage with defective bananas using screened lactic acid bacteria (LAB), sucrose, and tannase as additives. Eleven strains of LAB were isolated from the fruits and flowers of defective bananas, all of which were Gram-positive and catalase-negative bacteria that produced lactic acid from glucose. Among these LAB, homofermentative strain CG1 was selected as the most suitable silage additive due to its high lactic acid production and good growth in a low pH environment. Based on its physiological and biochemical properties and 16S rRNA gene sequence analysis, strain CG1 was identified as Lactiplantibacillus plantarum. Defective bananas contain 74.8–76.3% moisture, 7.2–8.2% crude protein, 5.9–6.5% ether extract, and 25.3–27.8% neutral detergent fibre on a dry matter basis. After 45 d of fermentation, the silage of deficient bananas treated with LAB or sucrose alone improved fermentation quality, with significantly (p < 0.05) lower pH and higher lactic acid contents than the control. The combination of LAB and sucrose had a synergistic effect on the fermentation quality of silage. The tannase-treated silage significantly (p < 0.05) decreased the tannin content, while the combination of tannase and LAB in silage not only decreased (p < 0.05) the tannin content, but also improved the fermentation quality. The study confirmed that defective bananas are rich in nutrients, can prepare good quality silage, and have good potential as a feed source for livestock.

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Microbial Co-occurrence Network and Fermentation Information of Natural Woody-Plant Silage Prepared With Grass and Crop By-Product in Southern Africa

Cited by 11 publications

References 42 publications

Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage

Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage

Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage

Characterization of Lactic Acid Bacteria Isolated from Banana and Its Application in Silage Fermentation of Defective Banana

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