Microbial community composition is consistent across anaerobic digesters processing wheat-based fuel ethanol waste streams

Town, Jennifer; Annand, Holly; Pratt, Dyan; Dumonceaux, Tim J.; Fonstad, Terrance A.

doi:10.1016/j.biortech.2014.01.074

Cited by 17 publications

(8 citation statements)

References 24 publications

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“…The high degree of change in the OBW inoculum, irrespective of the substrate, indicates that the methanogenic community in the inoculum had not reached steady state yet, since, in general, during stable anaerobic digestion a static methanogenic community is observed, which is not strongly influenced by a change in substrate (Shin et al ., 2010 ; Ritari et al ., 2012 ; De Vrieze et al ., 2013a,b ; Regueiro et al ., 2014 ; Town et al ., 2014 ; Venkatakrishnan et al ., 2014 ). The endurance of the Methanomicrobiales population in the treatment with the liquid manure substrate indicates that both the acetoclastic and hydrogenotrophic pathway are used for methane production from liquid manure.…”

Section: Discussionmentioning

confidence: 99%

Inoculum selection influences the biochemical methane potential of agro‐industrial substrates

Vrieze

Raport

Willems³

et al. 2015

Microbial Biotechnology

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Obtaining a reliable estimation of the methane potential of organic waste streams in anaerobic digestion, for which a biochemical methane potential (BMP) test is often used, is of high importance. Standardization of this BMP test is required to ensure inter-laboratory repeatability and accuracy of the BMP results. Therefore, guidelines were set out; yet, these do not provide sufficient information concerning origin of and the microbial community in the test inoculum. Here, the specific contribution of the methanogenic community on the BMP test results was evaluated. The biomethane potential of four different substrates (molasses, bio-refinery waste, liquid manure and high-rate activated sludge) was determined by means of four different inocula from full-scale anaerobic digestion plants. A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates. This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing. We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.

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Section: Discussionmentioning

confidence: 99%

Inoculum selection influences the biochemical methane potential of agro‐industrial substrates

Vrieze

Raport

Willems³

et al. 2015

Microbial Biotechnology

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“…Such methods allow more detailed understanding of the organization and behavior of these diverse communities [17,43,45,47,55,58]. However, data showing bacterial and archaeal community shifts during the OLR increase followed by ammonia and VFA inhibition have still been limited.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Organic Loading Rate Increase and the Presence of Zeolite on Microbial Community Composition and Process Stability During Anaerobic Digestion of Chicken Wastes

et al. 2015

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This study investigates the effect of the organic loading rate (OLR) increase from 1.0 to 3.5 g VS L(-1) day(-1) at constant hydraulic retention time (HRT) of 35 days on anaerobic reactors' performance and microbial diversity during mesophilic anaerobic digestion of ammonium-rich chicken wastes in the absence/presence of zeolite. The effects of anaerobic process parameters on microbial community structure and dynamics were evaluated using a 16S ribosomal RNA gene-based pyrosequencing approach. Maximum 12 % of the total ammonia nitrogen (TAN) was efficiently removed by zeolite in the fixed zeolite reactor (day 87). In addition, volatile fatty acids (VFA) in the fixed zeolite reactor accumulated in lower concentrations at high OLR of 3.2-3.5 g VS L(-1) day(-1). Microbial communities in the fixed zeolite reactor and reactor without zeolite were dominated by various members of Bacteroidales and Methanobacterium sp. at moderate TAN and VFA levels. The increase of the OLR accompanied by TAN and VFA accumulation and increase in pH led to the predominance of representatives of the family Erysipelotrichaceae and genera Clostridium and Methanosarcina. Methanosarcina sp. reached relative abundances of 94 and 57 % in the fixed zeolite reactor and reactor without zeolite at the end of the experimental period, respectively. In addition, the diminution of Synergistaceae and Crenarchaeota and increase in the abundance of Acholeplasmataceae in parallel with the increase of TAN, VFA, and pH values were observed.

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“…The authors concluded that the anaerobic digestion of thin stillage (the liquid fraction) can provide an additional energy recovery level of 41%, while wet cake (the solid fraction) has an energy recovery level of 57%. Town et al [25] also investigated the biochemical methane potential of wheat-based stillage. The results showed that the stillage as a whole produced 96% of the methane that was expected of it, while thin stillage produced 125% of the methane predicted, and the wet cake produced 95% of the methane estimated.…”

Section: Methane Recovery From Solid and Liquid Fractions Of Bioethanmentioning

confidence: 99%

Enhancing Bioenergy Yields from Sequential Bioethanol and Biomethane Production by Means of Solid–Liquid Separation of the Substrates

et al. 2019

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The production of second-generation ethanol using lignocellulosic feedstock is crucial in order to be able to meet the increasing fuel demands by the transportation sector. However, the technology still needs to overcome several bottlenecks before feasible commercialization can be realized. These include, for example, the development of cost-effective and environmentally friendly pretreatment strategies and valorization of the sidestream that is obtained following ethanol distillation. This work uses two chemical-free pretreatment methods—nitrogen explosive decompression (NED) and synthetic flue gas explosive decompression—to investigate the potential of a bioethanol production sidestream in terms of further anaerobic digestion. For this purpose, samples from different stages of the bioethanol production process (pretreatment, hydrolysis, and fermentation) and the bioethanol sidestream went through a separation process (involving solid–liquid separation), following which a biomethane potential (BMP) assay was carried out. The results show that both factors being studied in this article (involving the pretreatment method and the separation process) served to influence methane yields. Liquid fractions that were obtained during the process with NED gave rise to methane yields that were 8% to 12% higher than when synthetic flue gas was used; fermented and distillation sidestream gave rise to the highest methane yields (0.53 and 0.58 mol CH4/100 g respectively). The methane yields from the liquid fractions were between 60–88% lower than those that were obtained from solid fractions. Samples from the bioethanol sidestream (solid fraction) that were pretreated with NED had the highest methane yield (1.7 mol CH4/100 g). A solid–liquid separation step can be a promising strategy when it comes to improving the energy output from lignocellulosic biomass and the management of the ethanol distillation sidestream.

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Microbial community composition is consistent across anaerobic digesters processing wheat-based fuel ethanol waste streams

Cited by 17 publications

References 24 publications

Inoculum selection influences the biochemical methane potential of agro‐industrial substrates

Inoculum selection influences the biochemical methane potential of agro‐industrial substrates

Effect of the Organic Loading Rate Increase and the Presence of Zeolite on Microbial Community Composition and Process Stability During Anaerobic Digestion of Chicken Wastes

Enhancing Bioenergy Yields from Sequential Bioethanol and Biomethane Production by Means of Solid–Liquid Separation of the Substrates

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