Microbial community structure of anoxic–oxic-settling-anaerobic sludge reduction process revealed by 454-pyrosequencing

Zhou, Zhen; Weimin, Qiao; Xing, Chaoping; An, Ying; Shen, Xuelian; Ren, Weichao; Jiang, Lu-Man; Wang, Luochun

doi:10.1016/j.cej.2014.12.095

Cited by 121 publications

(42 citation statements)

References 38 publications

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“…As described, Propionvibrio was dominant among the phylotypes identified with the PAO primer set at the genus level, and it may or may not be a PAO (Albertsen et al 2016, Zhou et al 2015). For the eubacterial primers, Saccharibacteria spp.…”

Section: Resultsmentioning

confidence: 87%

“…While most other data sets did not exhibit the coverage to justify significant conclusions, Propionivibrio nonetheless was commonly observed (in many instances dominating; Tables S92). Functionally, Propionivibrio is a fermentative bacterium with the capability to produce short-chain VFAs (i.e., the optimal EBPR substrate) from polysaccharides (Zhou et al 2015); given these metabolic capabilities, certain species of Propionivibrio could be PAOs, although recent research has identified one potential species ( Candidatus Propionivibrio aalborgensis) as a putative GAO (Albertsen et al 2016). Other genera including Azonexus and Dechloromonas (within the Rhodocyclaceae family of the Betaproteobacteria class) were observed, as was Bdellovibrio (which is within the Bdellovibrionaceae family of the Deltaproteobacteria class).…”

Section: Resultsmentioning

confidence: 99%

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Characterizing and contrasting the microbial ecology of laboratory and full-scale EBPR systems cultured on synthetic and real wastewaters

2017

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The anthropogenic discharge of phosphorus (P) into surface waters can induce the proliferation of cyanobacteria and algae, which can negatively impact water quality. Enhanced biological P removal (EBPR) is an engineered process that can be employed to efficiently remove significant quantities of P from wastewater. Within this engineered system, the mixed microbial consortium (MMC) becomes enriched with polyphosphate accumulating organisms (PAOs). To date much knowledge has been developed on PAOs, and the EBPR process is generally well understood; nonetheless, the engineered process remains underutilized. In this study, investigations were conducted using qPCR and Illumina MiSeq to assess the impacts of wastewater (synthetic vs. real) on EBPR microbial ecology. While a strong relationship was demonstrated between EBPR metrics (P:C; influent VFA:P) and excellent P removal across diverse EBPR systems and MMCs, no such correlations existed with the specific MMCs. Moreover, MMCs exhibited distinct clusters based on substrate, and qPCR results based on the putative PAO Accumulibacter did not correlate with BLASTN eubacterial results for either Accumulibacter or Rhodocyclaceae. More critically, PAO-based sequences aligned poorly with Accumulibacter for both eubacterial and PAO primer sets, which strongly suggests that the conventional PAO primers applied in FISH and qPCR analysis do not sufficiently target the putative PAO Accumulibacter. In particular, negligible alignment was observed for PAO amplicons obtained from a MMC performing excellent EBPR on crude glycerol (an atypical substrate). A synthetic wastewater-based MMC exhibited the best observed BLASTN match of the PAO amplicons, raising concerns about the potential relevance in using synthetic substrates in the study of EBPR.

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Characterizing and contrasting the microbial ecology of laboratory and full-scale EBPR systems cultured on synthetic and real wastewaters

2017

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“…5). The evolution of microbial species to slow growers with low sludge yield under anaerobic conditions was proved by pyrosequencing (Zhou et al, 2015). Among slow-growing bacteria, enrichment of fermenters was observed in plants equipped with aerobiceanaerobic zones, with ability to degrade flocs and accelerate sludge decay (Goel and Noguera, 2006;Quan et al, 2012;Li et al, 2014).…”

Section: Bacterial Cells Do Not Decrease Under Anaerobic Conditionsmentioning

confidence: 99%

Concerning the role of cell lysis-cryptic growth in anaerobic side-stream reactors: The single-cell analysis of viable, dead and lysed bacteria

et al. 2015

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“…These techniques have significantly overcome the weakness of traditional methods, but only elucidate the major microbial groups in wastewater treatments. Recently, the metagenomic approach has emerged as one of the most effective and popular techniques for investigating microbial diversity and community in activated sludges [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Sequencing Batch Reactor and Bacterial Community in Aerobic Granular Sludge for Wastewater Treatment of Noodle-Manufacturing Sector

et al. 2018

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Abstract:The sequencing batch reactor (SBR) has been increasingly applied in the control of high organic wastewater. In this study, SBR with aerobic granular sludge was used for wastewater treatment in a noodle-manufacturing village in Vietnam. The results showed that after two months of operation, the chemical oxygen demand, total nitrogen and total phosphorous removal efficiency of aerobic granular SBR reached 92%, 83% and 75%, respectively. Bacterial diversity and bacterial community in wastewater treatment were examined using Illumina Miseq sequencing to amplify the V3-V4 regions of the 16S rRNA gene. A high diversity of bacteria was observed in the activated sludge, with more than 400 bacterial genera and 700 species. The predominant genus was Lactococcus (21.35%) mainly containing Lactococcus chungangensis species. Predicted functional analysis showed a high representation of genes involved in membrane transport (12.217%), amino acid metabolism (10.067%), and carbohydrate metabolism (9.597%). Genes responsible for starch and sucrose metabolism accounted for 0.57% of the total reads and the composition of starch hydrolytic enzymes including α-amylase, starch phosphorylase, glucoamylase, pullulanase, α-galactosidase, β-galactosidase, α-glucosidase, β-glucosidase, and 1,4-α-glucan branching enzyme. The presence of these enzymes in the SBR system may improve the removal of starch pollutants in wastewater.

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Microbial community structure of anoxic–oxic-settling-anaerobic sludge reduction process revealed by 454-pyrosequencing

Cited by 121 publications

References 38 publications

Characterizing and contrasting the microbial ecology of laboratory and full-scale EBPR systems cultured on synthetic and real wastewaters

Characterizing and contrasting the microbial ecology of laboratory and full-scale EBPR systems cultured on synthetic and real wastewaters

Concerning the role of cell lysis-cryptic growth in anaerobic side-stream reactors: The single-cell analysis of viable, dead and lysed bacteria

Sequencing Batch Reactor and Bacterial Community in Aerobic Granular Sludge for Wastewater Treatment of Noodle-Manufacturing Sector

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