Microbial Contamination inSpodoptera littoralisNuclear Polyhedrosis Virus Produced in Insects in Egypt

Role of Nucleopolyhedroviruses (NPVs) in the Management of Lepidopteran Pests in Asia

“…Although these studies on L. dispar NPV and C. pomonella GV failed to detect human pathogens in the product, these viruses were produced under temperate conditions, and it might be anticipated that production in a near tropical situation would involve greater microbiological contamination problems. Grzywacz et al (1997) quantified microbial contaminants level in S. littoralis NPV. They found 10 6 -10 9 bacterial colony forming units/ml in virus suspension containing 2.1 Â 10 9 POBs/ml.…”

Productivity and quality of polyhedral occlusion bodies of a nucleopolyhedrovirus harvested from Spodoptera litura (Lepidoptera: Noctuidae) larvae

“…The guideline for microbial pesticides proposed by the Ministry of Agriculture, Forestry and Fisheries of Japan does not show this kind of the limit but requests to describe methods of preventing a biological alien substance into the products (the Ministry of Agriculture, Forestry and Fisheries of Japan, 1997). The removal of microbial contaminants from the product, however, is expensive (Shapiro, 1982) and sometimes found to be difficult (Grzywacz et al, 1997). Microbial contamination in NPV-infected live larvae would be lower than that in NPV-killed larvae.…”

Mass Production of Entomopathogens in Less Industrialized Countries

“…Because BVs are produced in insects, there are issues around the microbial flora that occur in live insects and proliferate in dead insects. Several studies have characterized these conditions (Podgwaite et al, 1983;Grzywacz et al, 1997;Lasa et al, 2008). To date, none of the studies have identified human or veterinary pathogens, but such microbiological studies are an essential safety measure that should not be neglected.…”