Microbial surfaces in relation to pathogenicity.

Smith, H.

doi:10.1128/mmbr.41.2.475-500.1977

Cited by 192 publications

(134 citation statements)

References 88 publications

(127 reference statements)

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…The proteins located on the surfaces of Gram-negative bacteria are responsible for interactions of bacterial cells with the host tissue [1]. In this sense there-fore, physiologically active proteins associated with the bacterial envelope may play signi¢cant roles in destructive and protective events in the vicinity of bacteria in the infected areas.…”

Section: Introductionmentioning

confidence: 99%

Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis

Fujimura¹

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

Two arginine specific proteinases (Arg-gingipain [RGP-A, RGP-B]) and a lysine specific proteinase (Lys-gingipain [KGP]) were purified from materials of the envelope of Porphyromonas gingivalis solubilized by a detergent by the sequential procedures of ion-exchange chromatography, affinity chromatography, and isoelectric focusing. Each purified enzyme showed a single stained band on SDS-PAGE. The three enzymes were commonly activated by reducing reagents such as mercaptoethanol, dithiothreitol and cysteine. RGP-B was activated markedly by glycyl-glycine and KGP was activated significantly by EDTA. Both RGP-A and RGP-B were inhibited by p-hydroxymercuribenzoate, tosyl-L-lysine chloromethyl ketone (TLCK), N-ethylmaleimide, EDTA, leupeptin, L-trans-epoxy-succinylleucylamido-(4-guanidino)butane and antipain. KGP was inhibited by p-hydroxymercuribenzoate, N-ethylmaleimide and TLCK. The molecular masses of RGP-A and RGP-B were the same (43 kDa), and that of KGP was 48 kDa. The hydrolytic activities of RGPs and KGP to chromogenic synthetic substrates were limited to the compounds with arginine and lysine in the P-1 positions, respectively. When IgG was treated with the three enzymes separately, it was demonstrated that two new fragments of 34 kDa and 15 kDa were generated in each reaction product. Hemoglobin was almost exhaustively digested by RGP-B, but substantial degradation could not be induced by RGP-A or KGP treatment. z

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis

Fujimura¹

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…Survival and sporulation of only a few of the parasitic cells could provide further dissemination of the fungus. Smith (23) pointed out that, the more rapid the rate of multiplication, the more likely infection will be established, despite the activity of host defense mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Disseminated Coccidioidomycosis

Jitsukawa

Sato

Hayashi

et al. 1990

The Journal of Dermatology

View full text Add to dashboard Cite

We report a case of disseminated coccidioidomycosis in a 39-year-old Japanese male whose illness developed after returning from a trip to an endemic area. He showed positive coccidioidin skin reaction throughout the entire course of his illness. The primary lesion in the lung subsequently spread to the bone. While the patient was on treatment with 5-FC, he made another trip to the same endemic area. After this episode, he developed pulmonary symptoms and cutaneous nodules on his wrist. The possibility of reinfection with Coccidioides immitis is discussed. Electron microscopy of the cutaneous nodules revealed that the spherules examined maintained their structural integrity in the granulomatous lesion, suggesting the high viability of the organism. Host-parasitic interaction in coccidioidomycosis is discussed.

show abstract

“…ducreyi with host defence mechanisms such as serum and phagocytic leukocytes have been reported [1,2], little is known of the mechanisms by There is now considerable evidence that antigens on the surface of bacteria are frequently important determinants of virulence [3]. For this reason, recent research on the biology of H. ducreyi has centred on the structure of its cell wall.…”

Section: Although Investigations Of the Interaction Of Hmentioning

confidence: 99%

Identification of surface-exposed proteins Haemophilus ducreyi

Abeck

1987

FEMS Microbiology Letters

View full text Add to dashboard Cite

Microbial surfaces in relation to pathogenicity.

Cited by 192 publications

References 88 publications

Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis

Comparative properties of envelope-associated arginine-gingipains and lysine-gingipain of Porphyromonas gingivalis

Disseminated Coccidioidomycosis

Identification of surface-exposed proteins Haemophilus ducreyi

Contact Info

Product

Resources

About