Microbial Viability in Preparations Packaged for Single Use

Obayashi, Akiko; Oie, Shigeharu; Kamiya, Akira

doi:10.1248/bpb.26.667

Cited by 17 publications

(12 citation statements)

References 14 publications

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“…The results are shown as values of CFU/mL in semi-logarithmic graphs. As other experimental studies of microbial growth,5,12,13,21,22 the data obtained in this study were not analyzed statistically, because biological significance of these kinds of data is considered to be assessable without statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid

Kubo¹,

Shimono²,

Kaneda³

et al. 2010

Int. J. Med. Sci.

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Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions containing lipid. Methods: The pH value of a TPN solution containing lipid (pH 6.0, containing 20 ppm of NaHSO3) was adjusted by the addition of HCl to 5.7, 5.4, or 4.9. The pH value of another TPN solution (pH5.5, containing 400 ppm of NaHSO3) was adjusted by the addition of NaOH to 5.9, 6.3, or 6.8. A specific number of each microorganism was added to 10 mL of each test solution and incubated at room temperature. The number of microorganisms was counted as colony forming units at 0, 24, and 48 hrs later. Results: C albicans increased similarly at any pH values in the TPN solution. The bacterial species also increased rapidly at pH6.0 in the solution containing 20 ppm of NaHSO3, but growth was suppressed as the pH value was reduced, with growth halted at pH4.9. However, these bacterial species did not increase, even at pH5.9, in the other solution containing 400 ppm of NaHSO3. Conclusions: These results suggest that Candida species can grow rapidly in almost all TPN solutions regardless of the acidity, lipid, and NaHSO3; also, some bacterial species may grow in TPN solutions containing lipid unless the pH value is 5.0 or less. Therefore, each TPN solution should be examined whether or not the bacterial species can proliferate.

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Section: Methodsmentioning

confidence: 99%

Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid

Kubo¹,

Shimono²,

Kaneda³

et al. 2010

Int. J. Med. Sci.

View full text Add to dashboard Cite

show abstract

“…The results are shown as values of CFU/mL in semi-logarithmic graphs. Same as other experimental studies of microbial growth,14-18 the data obtained in this study were not analyzed statistically because biological significance of these kinds of data is considered assessable without statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

Effects of Lipid Emulsion and Multivitamins on the Growth of Microorganisms in Peripheral Parenteral Nutrition Solutions

Kubo¹,

Kaneda²,

Shimono³

et al. 2013

Int. J. Med. Sci.

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Background: Blood stream infections caused by Bacillus cereus or Serratia marcescens in patients receiving peripheral parenteral nutrition (PPN) have occasionally been reported in Japan, but these microorganisms are not major causes of blood stream infections in patients receiving total parenteral nutrition via a central venous catheter. In Japan, commercially available PPN solutions contain amino acids, glucose, and electrolytes, but not contain lipid emulsion (LE) and multivitamins (MV). In this study, the effects of LE and MV on the growth of microorganisms such as Bacillus cereus, Serratia marcescens, Staphylococcus aureus, and Candida albicans in PPN solutions were investigated. Methods: A commercial 3% amino acid and 7.5% glucose solution with electrolytes (AF) was used as the base solution to prepare test solutions (LAF, AFV, and LAFV) containing LE, MV, or both. Specifically, 20% LE was added to AF in a ratio of 1:9 to prepare LAF. MV was added to AF and LAF to prepare AFV and LAFV, respectively. A specified number of each microorganism was added to each 100 mL of AF, LAF, AFV, and LAFV in sterile plastic flasks, and all flasks were allowed to stand at room temperature. The number of colony forming units per mL of each microorganism was counted at 0, 24, and 48 hours after the addition of each microorganism. Results: Both Bacillus cereus and Serratia marcescens increased rapidly in AF as well as in LAF, AFV, and LAFV. Staphylococcus aureus did not increased in AF, but increased slightly in LAF and increased rapidly in AFV and LAFV. Candida albicans increased slightly in AF and increased rapidly in LAF, AFV, and LAFV. Conclusions: The results suggest the followings: if microbial contamination occurs, 1) Bacillus cereus and Serratia marcescens can grow rapidly in PPN solutions consisting of amino acids, glucose and electrolytes; 2) Staphylococcus aureus cannot grow without LE and MV, but can grow rapidly with MV; 3) Candida albicans can grow slowly without LE and MV, and the addition of LE or MV accelerates its growth.

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“…Obayashi et al [54] evaluated the microbial viability in 12 [138] blood products. Obayashi et al [54] evaluated the microbial viability in 12 [138] blood products.…”

Section: Nosocomial Infectionsmentioning

confidence: 99%

“…Viability of Serratia marcescens (a) and Burkholderia cepacia (b) at 30°C in (A) human serum albumin (), human haptoglobin (), and human antithrombin III freeze-dried concentrated (); in (B) polyglobin-N (), venoglobulin-IH (), gamma-venin P (),venilon-I (), globulin injection (), and ahlbulin (); and in (C) urokinase (), interferon-β (), and interferon-α ()[54].…”

mentioning

confidence: 99%

Microbiological and Endotoxin Contamination

Bohrer

2012

Sources of Contamination in Medicinal Products and Medical Devices

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Microbial Viability in Preparations Packaged for Single Use

Cited by 17 publications

References 14 publications

Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid

Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid

Effects of Lipid Emulsion and Multivitamins on the Growth of Microorganisms in Peripheral Parenteral Nutrition Solutions

Microbiological and Endotoxin Contamination

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