Microbiological Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation

Safwat, Osama; Elkateb, Mona Abdallah; Dowidar, Karin; Salam, Hala A. Abdel; Meligy, Omar El

doi:10.17796/1053-4628-42.1.3

Cited by 15 publications

(18 citation statements)

References 27 publications

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“…(-), <10 3 CFUÁmL À1 ; (þ), À10 3 CFUÁmL À1 ; (þþ), 10 4 CFUÁmL À1 ; (þþþ), 10 4 -10 5 CFUÁmL À1 ; (þþþþ), >10 5 CFU/mL. Our data agree with Safwat et al [11], who found that gaseous ozone had a strong antimicrobial effect on cariogenic bacteria both in vitro and ex vivo and can be used as an adjuvant in caries therapy, unlike other studies [13] that have shown that 20% of S. mutans, 6.7% of Fusobacterium nucleatum, 13.4% of Bifidobacterium spp. and 6.7% Eubacterium spp.…”

Section: Resultssupporting

confidence: 91%

“…A further study argues that 60 s ozone application results in the removal of most cariogenic microorganisms [10]. While the antibacterial properties of ozone and its role in creating suitable conditions for restoration of dental tissue mineral structures provide a strong argument for its use in the management of dental caries, some studies contradict these favourable results [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Types of microorganisms in proximal caries lesion and ozone treatment

Kirilova

Topalova-Pirinska

Kirov

et al. 2019

Biotechnology & Biotechnological Equipment

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The purpose of this clinical study was to investigate the types of microorganisms in deep proximal caries lesions and the efficacy of elimination of microorganisms after a 24 s ozone application to proximal cavity lesions prepared for restoration. Sixteen caries-active volunteers (female: 8, male: 8; age range: 35-55), with proximally situated deep caries lesions on premolars or molars, were included in the study. Each patient underwent two microbiological investigations. First, the caries decay before treatment was assessed. Second, the dentine tissue was assessed after the removal of necrotic tooth decay and 24 s ozone application. The prevalence of oral streptococci was determined. In addition, we isolated Candida albicans, Enterococcus faecalis and Peptostreptococcus spp. from deep proximal caries lesion in caries-active patients. All microorganisms found in the deep proximal caries lesions were destroyed following 24 s ozone application. Our findings suggest that S. anginosus group, C. albicans and E. faecalis should be considered as an index for caries activity in caries-active patients, but additional studies are necessary to confirm this suggestion. This study demonstrated that 24 s of gaseous ozone application to the deep proximal caries lesions effectively eliminated microbial species.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Types of microorganisms in proximal caries lesion and ozone treatment

Kirilova

Topalova-Pirinska

Kirov

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Dentistry is constantly searching for treatments that cause less discomfort to patients. This is also important for dentists working in the context of the pandemic, which requires professional preventive and minimally invasive approaches to the management of caries ( 7 ).New technologies such as cavity preparation by oscillation (ultrasound) ( 8 ), lasers ( 9 ), ozone ( 10 ), and chemomechanical caries removal agents ( 11 ) have been used. Most of these methods involve a high cost and may lead to tooth hypersensitivity, contributing to painful sensations, especially in children.Chemomechanical caries removal is a non-invasive technique that aims to dissolve necrotic tissues, facilitating the removal of the tissue softened manually with blunt-tip instruments.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study

Guedes¹,

Bonvicini²,

Souza³

et al. 2021

J Clin Exp Dent

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Background The use of chemomechanical agents for caries removal has been indicated as a non-invasive treatment option; however, their possible deleterious effects on the dental-pulp complex have been insufficiently studied. This study assessed the direct cytotoxicity of two chemomechanical caries removal agents (Brix 3000™ - BX and Papacarie Duo™ - PD) on pulp cells from deciduous teeth, as well as to assess the morphology and chemical compositions of the dentin surface after the application of these materials. Material and Methods The cells were seeded (50,000 cells/cm²) in a culture medium (DMEM with 10% fetal bovine serum - FBS). After 24 hours, the BX and PD materials were added to 1:20, 1:100, and 1:1000 dilutions. Non-exposed cells were considered as the control group. The viability test (MTT), Trypan Blue assay (TB), and cell morphology (Scanning Electron Microscopy - SEM) were performed after 24 hours of agent application. For the SEM and chemical (energy-dispersive X-ray spectrometry - EDS) dentin evaluation, 0.3-mm-thick dentin discs were obtained and divided into control group (no treatment) and surfaces covered with 37% phosphoric acid, BX, or PD. Data were compared by one-way ANOVA and Tukey’s test ( p <0.05). Results Decreases in cell viability and numbers of viable cells were observed for both materials, at all dilutions, when compared with the control group ( p <0.05). The BX and PD materials did not cause visually perceptible changes, according to SEM, on the surfaces of dentin discs. The EDS analysis did not indicate a statistically significant difference in the levels of calcium (Ca) and phosphorus (P) between the materials and the control group ( p >0.05). Conclusions Both materials showed cytotoxicity when in direct contact with the pulp cells from deciduous teeth, and the BX material presented lower cytotoxicity than the PD material. Moreover, both materials did not significantly change the dentin composition. Key words: Cell culture, cytotoxicity, dental pulp, papacarie, primary teeth.

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“…Baseado nisso, observa-se uma incoerência a respeito dos protocolos para implementação da ozonioterapia, que pode ser explicada devido a uma falta de estudos na área e a dificuldade de se trabalhar com o gás em ambiente clínico, por conta de sua instabilidade. Porém, como estratégia de gerenciamento alternativo, o ozônio continua sendo recomendado devido à sua poderosa capacidade de inativar microrganismos, somado ao fator de haver evidências crescentes de que ele pode ser empregado como um agente terapêutico útil no tratamento odontológico, seja em relação à periodontite associada a bactérias, vírus e fungos (Kshitish & Laxman, 2010), estomatite protética (Muller et al, 2007), irrigação de canais radiculares (Cardoso et al, 2008), limpeza da cavidade após remoção da biomassa cariogênica (Safwat et al, 2018) ou desinfecção de matérias de uso clinico (Savabi et al, 2018).…”

Section: Discussionunclassified

Avaliação da ação antifúngica da ozonioterapia sobre diferentes espécies de Candida para aplicação na cavidade oral – Revisão de Literatura

Roque

Loth

Baeza

2021

RSD

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O gênero Candida é o mais comumente associado ao desenvolvimento de infecção nos tecidos orais, denominada candidose oral. O manejo terapêutico dessa patologia é bem complexo e inclui a utilização de agentes antifúngicos de aplicação tópica ou sistêmica, dependendo da severidade da infecção. Nesse contexto, o objetivo desse estudo foi, por meio de uma revisão de literatura, avaliar a ação antifúngica do ozônio sobre leveduras do gênero Candida, visando aplicação em cavidade oral, pelo fato de o ozônio ser sugerido como terapia alternativa devido a sua ação oxidante. Para tanto, foi realizada busca pelo termo “ozone and Candida” nas bases de dados PubMed, SciELO e BVS por artigos publicados até abril de 2020. Após avaliação foram selecionados 23 artigos que se enquadram nos critérios. Pode-se concluir que o ozônio apresenta uma significante ação antimicrobiana sobre leveduras do gênero Candida. O presente estudo sugere que o seu uso tem a possibilidade de atenuar a resposta curativa dessa infecção, visto que inúmeros estudos tenham relatado melhora nos quadros infecciosos e diminuição de unidades formadoras de colônia. Todavia, novas investigações e comparações de perfil de toxicidade são necessários para estabelecer corretos protocolos de uso clínico.

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Microbiological Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation

Abstract: Ozone gas had a significant antimicrobial effect in deep class I carious lesions.

Cited by 15 publications

References 27 publications

Types of microorganisms in proximal caries lesion and ozone treatment

Types of microorganisms in proximal caries lesion and ozone treatment

Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary in vitro study

Avaliação da ação antifúngica da ozonioterapia sobre diferentes espécies de Candida para aplicação na cavidade oral – Revisão de Literatura

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