Microbiota associations with inflammatory pathways in asthma

Wang, Juan; Chai, Jianmin; Zhang, Linlin; Zhang, Lijiao; Yan, Wei; L, Sun; Chen, Yahong; Sun, Yongchang; Zhao, Jiangchao; Chang, Chun

doi:10.1111/cea.14089

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…In this study, the results of 16S rDNA amplicon sequencing showed that the AR mice had a higher relative abundance of Proteobacteria and a lower abundance of Actinobacteria than the naïve and MFXD groups. Similar to our results, Wang et al (2022) revealed that in patients with asthma, the abundance of Actinobacteria may be negatively associated with neutrophils and participate in airway inflammation. Proteobacteria was also increased in patients with asthma, which may correlate with bronchial hyperresponsiveness.…”

Section: Discussionsupporting

confidence: 92%

Mahuang Fuzi Xixin decoction ameliorates allergic rhinitis and repairs the airway epithelial barrier by modulating the lung microbiota dysbiosis

Wei,

Ding,

Liang

et al. 2023

Front. Microbiol.

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BackgroundAllergic rhinitis (AR) is a common disorder, that burdens general well-being. Although the lung is connected to the upper respiratory tract, which is rich in microorganisms, no studies have reported the relationship between lung microbiota and AR. Mahuang Fuzi Xixin decoction (MFXD) is a traditional Chinese medicine (TCM) formula that is widely used to treat AR in the clinic but its underlying mechanism remains unclear.HypothesisWe hypothesized that lung microbiota is associated with the pathogenesis of AR, and MFXD can improve AR by regulating microbiota dysbiosis.MethodsThe ovalbumin-induced mouse AR model was used to evaluate the therapeutic effect of MFXD on AR. Then 16S rDNA amplicon sequencing, untargeted metabolomics, and other molecular biology technology were used to clarify the effects of MFXD on lung microbes dysbiosis and AR progression. Further, the human nasal epithelial cell line (HNEpCs) was used to evaluate the protective effect of MFXD on epithelial barrier damage caused by specific pathogens.ResultsMFXD decreased plasma histamine and IgE levels, ameliorated pathological damage, and diminished the expression of tight junction proteins (ZO-1 and occludin) in lung and nasal tissues. MFXD altered AR-induced microbiota dysbiosis in the lungs and also plasma metabolites. Oral administration of MFXD altered microbiota dysbiosis in lung and AR-associated metabolic disorders. The dominant bacteria in the lungs of AR mice damaged the airway barrier, and MFXD reversed this change.ConclusionThis study revealed the correlation between the lung microbiota and AR in the mice model. We confirmed that lung microbiota plays a vital role in AR and that MFXD reduced damage to the epithelial barrier of the lungs and nasal mucosa by regulating lung microbiota and plasma metabolism imbalances. Our research provides a reference for the effect of lung microbiota on AR and provides a new idea for the treatment of AR.

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Section: Discussionsupporting

confidence: 92%

Mahuang Fuzi Xixin decoction ameliorates allergic rhinitis and repairs the airway epithelial barrier by modulating the lung microbiota dysbiosis

Wei,

Ding,

Liang

et al. 2023

Front. Microbiol.

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“…The overall Inuit OP microbiome composition was consistent with other studies where the four predominant phyla found here (Firmicutes, Actinobacteria, Bacteroidetes and Protobacteria) were also reported either in healthy individuals or in people with various respiratory diseases 13 , 16 , 23 , 32 . Streptococcus was the most abundant genus in our samples, a finding consistent with its predominance in oral, bronchial and lung tissues 13 .…”

Section: Discussionsupporting

confidence: 91%

Distinctive features of the oropharyngeal microbiome in Inuit of Nunavik and correlations of mild to moderate bronchial obstruction with dysbiosis

Flahaut,

Leprohon,

Pham

et al. 2023

Sci Rep

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Inuit of Nunavik are coping with living conditions that can influence respiratory health. Our objective was to investigate associations between respiratory health in Inuit communities and their airway microbiome. Oropharyngeal samples were collected during the Qanuilirpitaa? 2017 Inuit Health Survey and subjected to metagenomic analyses. Participants were assigned to a bronchial obstruction group or a control group based on their clinical history and their pulmonary function, as monitored by spirometry. The Inuit microbiota composition was found to be distinct from other studied populations. Within the Inuit microbiota, differences in diversity measures tend to distinguish the two groups. Bacterial taxa found to be more abundant in the control group included candidate probiotic strains, while those enriched in the bronchial obstruction group included opportunistic pathogens. Crossing taxa affiliation method and machine learning consolidated our finding of distinct core microbiomes between the two groups. More microbial metabolic pathways were enriched in the control participants and these were often involved in vitamin and anti-inflammatory metabolism, while a link could be established between the enriched pathways in the disease group and inflammation. Overall, our results suggest a link between microbial abundance, interactions and metabolic activities and respiratory health in the Inuit population.

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“…Initial studies of the respiratory microbiota in humans showed the microbial composition and community structure of the lower airways were significantly different between asthmatic and healthy humans; however, these studies considered asthma as a single entity ( 17 , 37 , 54 ). More recently, the microbiota composition has been associated with disease severity and specific inflammatory pathways in humans with asthma underscoring the importance of recognizing phenotypic and endotypic characteristics ( 7 , 55 – 57 ). Whether cats with different phenotypes have yet unrecognized differences in endotypes deserves further study with a more thorough immunologic evaluation but could be supported by the heterogeneity between bacterial taxa within the asthmatic cats of this study.…”

Section: Discussionmentioning

confidence: 99%

Respiratory dysbiosis in cats with spontaneous allergic asthma

et al. 2022

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Deviations from a core airway microbiota have been associated with the development and progression of asthma as well as disease severity. Pet cats represent a large animal model for allergic asthma, as they spontaneously develop a disease similar to atopic childhood asthma. This study aimed to describe the lower airway microbiota of asthmatic pet cats and compare it to healthy cats to document respiratory dysbiosis occurring with airway inflammation. We hypothesized that asthmatic cats would have lower airway dysbiosis characterized by a decrease in richness, diversity, and alterations in microbial community composition including identification of possible pathobionts. In the current study, a significant difference in airway microbiota composition was documented between spontaneously asthmatic pet cats and healthy research cats mirroring the finding of dysbiosis in asthmatic humans. Filobacterium and Acinetobacter spp. were identified as predominant taxa in asthmatic cats without documented infection based on standard culture and could represent pathobionts in the lower airways of cats. Mycoplasma felis, a known lower airway pathogen of cats, was identified in 35% of asthmatic but not healthy cats. This article has been published alongside “Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma” (1).

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Microbiota associations with inflammatory pathways in asthma

Cited by 8 publications

References 40 publications

Mahuang Fuzi Xixin decoction ameliorates allergic rhinitis and repairs the airway epithelial barrier by modulating the lung microbiota dysbiosis

Mahuang Fuzi Xixin decoction ameliorates allergic rhinitis and repairs the airway epithelial barrier by modulating the lung microbiota dysbiosis

Distinctive features of the oropharyngeal microbiome in Inuit of Nunavik and correlations of mild to moderate bronchial obstruction with dysbiosis

Respiratory dysbiosis in cats with spontaneous allergic asthma

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