Microchamber microfluidics combined with thermogellable glycomicrogels – Platform for single cells study in an artificial cellular microenvironment

Student, Sebastian; Milewska, Małgorzata; Ostrowski, Ziemowit; Gut, K.; Wandzik, Ilona

doi:10.1016/j.msec.2020.111647

Cited by 7 publications

(6 citation statements)

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“… 22 , 106 Recently, microgels were used as soft matrices for 3D cell culture 99 or in microfluidic microchambers. 107 …”

Section: Applications Of Trehalose Materialsmentioning

confidence: 99%

“…22,106 Recently, microgels were used as soft matrices for 3D cell culture 99 or in microfluidic microchambers. 107 As with other hydrogel drug delivery systems, these trehalose-containing hydrogels and nanogels offer tunable release and degradation delivery matrices. Even the multifunctionalized trehalose used as monomers and cross-linkers still provide stabilization for the encapsulated proteins.…”

Section: Conjugatesmentioning

confidence: 99%

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Synthesis and Application of Trehalose Materials

Vinciguerra

Gelb

Maynard

2022

JACS Au

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Trehalose is a naturally occurring, nonreducing disaccharide that is widely used in the biopharmaceutical, food, and cosmetic industries due to its stabilizing and cryoprotective properties. Over the years, scientists have developed methodologies to synthesize linear polymers with trehalose units either in the polymer backbone or as pendant groups. These macromolecules provide unique properties and characteristics, which often outperform trehalose itself. Additionally, numerous reports have focused on the synthesis and formulation of materials based on trehalose, such as nanoparticles, hydrogels, and thermoset networks. Among many applications, these polymers and materials have been used as protein stabilizers, as gene delivery systems, and to prevent amyloid aggregate formation. In this Perspective, recent developments in the synthesis and application of trehalose-based linear polymers, hydrogels, and nanomaterials are discussed, with a focus on utilization in the biomedical field.

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“… 22 , 106 Recently, microgels were used as soft matrices for 3D cell culture 99 or in microfluidic microchambers. 107 …”

Section: Applications Of Trehalose Materialsmentioning

confidence: 99%

Section: Conjugatesmentioning

confidence: 99%

Synthesis and Application of Trehalose Materials

Vinciguerra

Gelb

Maynard

2022

JACS Au

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“…[16][17][18] Trehalose incorporation has also been found to impart beneficial characteristics to macromolecular non-viral transfection agents such as pDNA, 19 cryopreservatives, 20 anti-adhesives to inhibit bacterial infections 21,22 and hydrogel matrices for 3D cell culture. 23,24 The activity of trehalose glycopolymers is usually investigated in the context of structural differences in these macromolecules, including the length of the linker structure between the trehalose moiety and the polymer chain, 14 the type of trehalose functionalization, 8 the position of functional group(s) on trehalose, 9 macromolecular topology, 25 molecular weight, 26 or the ratio of sugar to non-sugar moieties. 13,18 However, the uniqueness of trehalose glycopolymers is rather poorly verified, because they are rarely compared with glycopolymers of other disaccharides, and even when they are, the compared glycopolymers usually differ in the linker structure between disaccharide moieties or in the functionalization approach, which may significantly influence the overall macromolecular properties.…”

Section: Introductionmentioning

confidence: 99%

Structurally analogous trehalose and sucrose glycopolymers – comparative characterization and evaluation of their effects on insulin fibrillation

et al. 2022

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“…Most PDMS-based active degassing methods use vacuum microchambers for bubble extraction [ 34 , 35 ], which is usually implemented in a multi-layered structure, making device fabrication difficult and impractical. As a simple alternative, in-plane degassing methods has been developed for PDMS-based microfluidic devices [ 36 , 37 , 38 , 39 ]. In this case, it is necessary to place degassing lines near the microchannels where air bubbles form frequently.…”

Section: Introductionmentioning

confidence: 99%

Lateral Degassing Method for Disposable Film-Chip Microfluidic Devices

et al. 2021

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It is critical to develop a fast and simple method to remove air bubbles inside microchannels for automated, reliable, and reproducible microfluidic devices. As an active degassing method, this study introduces a lateral degassing method that can be easily implemented in disposable film-chip microfluidic devices. This method uses a disposable film-chip microchannel superstrate and a reusable substrate, which can be assembled and disassembled simply by vacuum pressure. The disposable microchannel superstrate is readily fabricated by bonding a microstructured polydimethylsiloxane replica and a silicone-coated release polymeric thin film. The reusable substrate can be a plate that has no function or is equipped with the ability to actively manipulate and sense substances in the microchannel by an elaborately patterned energy field. The degassing rate of the lateral degassing method and the maximum available pressure in the microchannel equipped with lateral degassing were evaluated. The usefulness of this method was demonstrated using complex structured microfluidic devices, such as a meandering microchannel, a microvortex, a gradient micromixer, and a herringbone micromixer, which often suffer from bubble formation. In conclusion, as an easy-to-implement and easy-to-use technique, the lateral degassing method will be a key technique to address the bubble formation problem of microfluidic devices.

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Microchamber microfluidics combined with thermogellable glycomicrogels – Platform for single cells study in an artificial cellular microenvironment

Cited by 7 publications

References 50 publications

Synthesis and Application of Trehalose Materials

Synthesis and Application of Trehalose Materials

Structurally analogous trehalose and sucrose glycopolymers – comparative characterization and evaluation of their effects on insulin fibrillation

Lateral Degassing Method for Disposable Film-Chip Microfluidic Devices

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