Microcirculatory and trophic effects of short chain fatty acids in the human rectum after Hartmann's procedure

Mortensen, Frank Viborg; Hessov, I; Birke, H.; Korsgaard, Niels; Nielsen, Henning Κ.

doi:10.1002/bjs.1800781019

Cited by 65 publications

(32 citation statements)

References 24 publications

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“…SCFAs cause greater blood flow to the intestinal wall through diminishing the resistance of the arterioles of the colon and rectum. They promote adhesion between the colonocytes, increase sodium and water absorption, stimulate normal growth of the colon epithelial cells, influence motility and favor healing of intestinal wall lesions 15,16,17 . SCFAs play a protective role in relation to neoplasia development because their antiproliferative action interferes in the cell differentiation process, thereby favoring apoptosis 17,18 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

et al. 2008

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Purpose:To evaluate histopathological alterations of the colon wall in segments with and without intestinal transit, by computer-assisted imaging, and to correlate these with the length of time diversion. Methods: Thirty male Wistar rats were subjected to intestinal transit diversion by a proximal colostomy and distal mucosa fistula. The animals were divided into three experimental groups according to how long after the initial surgical procedure they were sacrificed: six, twelve and eighteen weeks. Colon segments with and without transit were subjected to histopathological study. The variables colon crypt length, mucosal ulceration, muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria, vascular congestion, number of caliciform cells, inflammatory grade and degree of inflammation, comparing the two colon segments in the different experimental groups were studied. Intestinal crypt length, muscle layer thickness of the mucosa, submucosa and muscularis propria and caliciform cells were measured by computer-assisted imaging method. Mean equality, variance analysis and correlation tests were used in the statistical analysis, and the significance level was set at 5%. Results:Comparison between segments with and without transit showed that the latter presented reduced length of colon crypts and increased muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria. There were greater quantities of ulceration of the mucosal and greater degree of inflammation with increasing time without transit. Mucosal ulceration, submucosal vascular congestion, increased thickness of the submucosal and muscularis propria layers, presence of caliciform cells, inflammatory infiltrate and inflammatory grade correlated significantly with the length of time without transit. Conclusions: Histological alterations occurred in all layers of the colon wall, in the segments without intestinal transit. Ulcerations in the intestinal mucosa, increased number of caliciform cells, greater vascular congestion of the submucosal layer and inflammatory reaction were related to increasing length of time without transit. Key words: Colon. Colitis. Colostomy. Image Processing, Computer-Assisted. Rats. RESUMOObjetivo: Avaliar por método de imagem assistida por computador as alterações histopatológicas da parede cólica em segmentos providos e desprovidos de trânsito intestinal e relacioná-las ao tempo de exclusão. Métodos: Trinta ratos Wistar machos foram submetidos à derivação do trânsito no cólon esquerdo por meio de colostomia proximal e fístula mucosa distal. Os animais foram divididos em três grupos experimentais segundo o sacrifício ter sido realizado seis, doze e dezoito semanas após o procedimento cirúrgico inicial. Segmentos dos cólons providos e desprovidos de trânsito foram submetidos a estudo histopatológico. Foram analisadas as variáveis: comprimento das criptas cólicas, ulceração na mucosa, espessura das camadas muscular da mucosa, submucosa e muscular própria, congestão vascular, número...

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Section: Discussionmentioning

confidence: 99%

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

et al. 2008

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“…te: aumento da proliferação celular da mucosa colô-nia, acelerando a reepitelização e aumento do fluxo sangüíneo, com conseqüente incremento da oferta de oxigênio e aceleração da maturação do colágeno 15 . Friedel e Levine 16 mantiveram ratos em nutrição parenteral total, recebendo infusões de AGCC no cólon proximal e observaram aumento de massa e de DNA da mucosa, sem contudo verificar alterações na função absortiva da víscera .…”

Section: Greca Et Alunclassified

Os ácidos graxos de cadeia curta na cicatrização de anastomoses colônicas: estudo experimental em ratos

Greca

Biondo-Simões

Martins³

et al. 2003

Rev. Col. Bras. Cir.

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RESUMO:Objetivo: Avaliar a ação dos ácidos graxos de cadeia curta (AGCC) na cicatrização de anastomoses colônicas em ratos. Método: Utilizaram-se 50 ratos divididos em 4 grupos. Dois desses grupos, vinte e seis animais, foram submetidos à operação de Hartmann; metade destes (grupo HF) recebeu infusões retais diárias, no pós-operatório, de solução fisiológica a 0,9% ; a outra metade (grupo HA) recebeu solução de butirato de sódio à 80mmol/l (AGCC) (HA). Os dois grupos restantes, vinte e quatro animais foram submetidos à anastomose colônica término-terminal a 4 cm da borda anal. Destes, 12 animais (grupo AF) receberam infusões retais de soro fisiológico a 0,9% e 12 animais (grupo AA) receberam a solução com AGCC. Os animais foram avaliados no 7º e 14º dia do pós-operatório. Foi realizada análise histológica da densitometria do colágeno pela coloração do sirius red, utilizandose a microscopia de polarização com método computadorizado. Resultados: Comparando-se os grupos submetidos à cirurgia de Hartmann, portanto na ausência do trânsito intestinal, observou-se concentração significativamente maior de colágeno I e total no grupo que recebeu AGCC (p=0,001). Comparando-se os grupos submetidos à anastomose término-terminal, com interferência do trânsito intestinal, não se encontrou diferença significante da concentração de colágeno I e total (p=0,056 e p=0,397, respectivamente). A ação isolada do trânsito intestinal promoveu também aumento da produção de colágeno, quando comparado ao grupo sem atuação do trânsito intestinal. Conclusão: A administração via retal de AGCC, na ausência do trânsito intestinal mostrou-se de grande valia, promovendo aumento da síntese do colágeno. Independentemente da interferência do trânsito intestinal, o principal efeito do AGCC esteve relacionado com o aumento da maturação do colágeno. Descritores: Ácidos graxos voláteis INTRODUÇÃOAlém da absorção de água, com eficiên-cia de 99%, o cólon participa na absorção de sódio, cloro, amônia, vitaminas e ácidos graxos de cadeia curta. Estas substâncias estão relacionadas com a manutenção do pH intestinal, com trofismo da mucosa colônica e proteção da microbiota normal, evitando o crescimento de bactérias patogênicas.

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“…Mucus plays the important role of colonic protection against luminal pathogens and toxins. Indeed, short chain fatty acids enemas are expected to be a therapeutic alterative in patients with diversion colitis or distal ulcerative colitis either by providing an energy source for colonocytes or by stimulating the renewal of injured epithelium (18,21). However, Tarrerias et al (22) reported that the beneficial effect of short chain fatty acid (especially butyrate) enemas on hypersensitivity and inflammation in inflammatory bowel disease is questionable in the TNBS-induced colitis model.…”

Section: Discussionmentioning

confidence: 99%

Milk Whey Culture With Propionibacterium freudenreichii ET-3 Is Effective on the Colitis Induced by 2,4,6-Trinitrobenzene Sulfonic Acid in Rats

Uchida¹,

Mogami²

2005

J Pharmacol Sci

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Abstract. This study aimed to evaluate whether milk whey culture with Propinibacterium freudenreichii ET-3 (milk whey culture), which has been reported to have Bifidogenic activity, is effective on the colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in rats. For the induction of colitis, the colon was clamped and 0.1 M TNBS in 35% ethanol was injected into the luminal side of the clamped portion under pentobarbital anesthesia. From the next day of colitis induction, milk whey culture was administered orally at doses of 1 and 3 g / kg, twice a day for 9 days. On the 10th day, rats were sacrificed and ulcer size was measured. Milk whey culture significantly accelerated the healing of the colitis in a dose-dependent manner, but culture medium did not. To clarify the active substance, the effects of propionic acid and acetic acid contained in milk whey culture was tested. Sodium propionate significantly accelerated the healing of TNBS-induced colitis, but sodium acetate did not. The above results show that milk whey culture may become a useful prebiotic for the therapy of inflammatory bowel disease and that propionic acid may be one of the active substances contained in milk whey culture.

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Microcirculatory and trophic effects of short chain fatty acids in the human rectum after Hartmann's procedure

Cited by 65 publications

References 24 publications

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

Os ácidos graxos de cadeia curta na cicatrização de anastomoses colônicas: estudo experimental em ratos

Milk Whey Culture With Propionibacterium freudenreichii ET-3 Is Effective on the Colitis Induced by 2,4,6-Trinitrobenzene Sulfonic Acid in Rats

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