Microencapsulation of Lemongrass Leaves Effect on Reactive Oxygen Species (ROS) Fibroblasts

Veronica, Gisca; Komariah,; Maria, Ln Gabriella Clara

doi:10.1109/inhence52833.2021.9537219

Cited by 3 publications

(5 citation statements)

References 37 publications

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“…This non enzymatic antioxidant also donated its electrons, which cause free radicals became a more stable molecules and also breaking the chained reactions [31]. This result supports the findings of Veronica et al [32],…”

Section: N Et Alsupporting

confidence: 79%

The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress

et al. 2022

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Objective: The purpose of this research is to assess the antioxidant activity of lemongrass leaves extract in terms of lowering ROS generation and its effect on the viability and proliferation of fibroblasts under oxidative stress. Material and Methods: The antioxidant activity was measured using the DPPH method and the ROS assay was carried out by fluorescent H2DCFDA staining. Viability and proliferation assays were performed using the Cell Counting Kit-8 (CCK-8) and was read at 450 nm using microplate reader. The groups were divided into 8, namely fibroblasts without treatment (comparison group), fibroblast induced by H 2 O 2 (negative control), fibroblast with H 2 O 2 then treated with ascorbic acid (positive control), and fibroblast with H 2 O 2 then treated with lemongrass leaves extract at various concentrations (10, 20, 30, 40, and 50 ppm). Results: The results showed that the antioxidant activity of lemongrass leaves extract had an IC 50 value of 64.17 ppm. ROS production were reduced by the LgLE of all concentrations if compared with negative control (p=0.819). LgLE can maintained the fibroblast viability with 10 ppm of LgLE was the most optimum concentration (p<0.05). LgLE can induced the proliferation of fibroblast, with the most effective was at 24 h of observation (p<0.05). Conclusion: Lemongrass leaves extract has a strong antioxidant activity that can reduce oxidative stress and increase the viability and proliferation of fibroblasts with the optimum concentration is at 10 ppm.

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Section: N Et Alsupporting

confidence: 79%

The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress

et al. 2022

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“…Sebagai respon terhadap luka, tubuh mengalami proses penyembuhan (Gonzales et al, 2016). Foto hasil pengamtan dianalisis untuk menghitung fraksi keseluruhan dari sel terflouresensi hijau menggunakan Image J Software (Veronica et al, 2021).…”

Section: Pendahuluanunclassified

Penurunan Produksi Reactive Oxygen Species (ROS) Fibroblas dengan Nano Kitosan Kumbang Tanduk (Xylotrupes gideon)

Komariah

Priscilla²,

Wahyudi³

et al. 2023

JPS

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Fibroblas berperan dalam proses penyembuhan luka dengan pembentukan pembuluh darah, penggerakan serta proliferasi, deposit matriks ekstraseluler dan remodeling jaringan. Pada penyembuhan luka, netrofil dan makrofag akan mengalami peningkatan penggunaan oksigen sehingga memproduksi ROS, peningkatan produk ini akan menyebabkan stres oksidatif pada fibroblas yang akan mempengaruhi proses migrasi dan proliferasi selama proses penyembuhan luka. Kandungan gugus amino (-NH2) dan gugus hidroksil (-OH) pada nano kitosan mampu mengurangi stres oksidatif fibroblas. Penelitian bertujuan untuk mengetahui kemampuan nano kitosan X. gideon dalam menurunkan produksi ROS fibroblas. Penelitian terbagi menjadi enam kelompok terdiri dari kontrol negatif (H2O2), kontrol pembanding, kontrol asam askorbat, nano kitosan konsentrasi 200, 400, dan 600 µg/mL. Produksi ROS dilihat menggunakan probe 2′,7′-Dichlorodihydrofluorescein diacetate (H2DCF-DA). Produksi fibroblas diperlihatkan dengan intensitas fibroblas terflouresen hijau dihitung menggunakan software image J. Rerata produksi ROS pada kelompok nano kitosan 200, 400, dan 600 µg/mL berbeda signifikan dengan kontrol negatif (p<0,05), sedangkan yang diberikan asam askorbat tidak signifikan (p>0,05) dengan perlakuan 200 dan 600 µg/mL, namun signifikan dengan nano kitosan 400 µg/mL. Nano kitosan X. gideon mampu menurunkan produksi ROS fibroblas pada konsentrasi 400 µg/mL.

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“…Chitosan with 83% degrees of deacetylation and a weight value of 0.5 g was dissolved in 1% acetic acid (Merck, Germany). Subsequently, 2 mL of 10% lemongrass leaf extract (LE) and 100 mL of distilled water were added (Veronica et al 2021). Stirring was conducted using a magnetic stirrer (IKA RH basic 2, Germany) and heating at 40 °C with a speed of 2,500 rpm for 20 min and was subsequently carried out without heating for 100 min.…”

Section: The Preparation Of Le-loaded On Chitosan X Gideon (Lechxg)mentioning

confidence: 99%

“…Herbal medicine for the ulcer healing process has been widely used, such as lemongrass (Cymbopogon cit ratus DC) (Veronica et al 2021). Lemongrass is one of the spices growing in the tropics and is widely used in Southeast Asia, including Indonesia (Maria et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…by protecting the active compound from oxidation to improve its therapeutic potential (Negi and Kesari 2022). The common polymer material used as a trapping matrix for encapsulation is chitosan (Andikop utri et al 2021), which is a natural polymer compound ob tained from insect exoskeleton (Baharlouei and Rahman 2022), such as horn beetle (Xylotrupes gideon) (Veronica et al 2021). Thus, it can be developed to facilitate a drug delivery system due to its biocompatible, biodegradable, low toxicity level (Agarwal et al 2018), and simple prepa ration method (Mohammed et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Komariah,

Trisfilha,

Wahyudi

et al. 2023

Indones. J. Biotechnol.

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During phagocytosis, phagocyte cells discharge reactive oxygen species referred to as respiratory bursts, inducing a rise in pro‐oxidants and subjecting the cell to oxidative stress. Such stress is a biological mechanism related to an imbalance in pro‐oxidant/antioxidant homeostasis, which generates toxic reactive oxygen. Encapsulation is a coating process to improve the stability of bioactive compounds from lemongrass extract. Therefore, this study aims to determine the encapsulation activity of lemongrass leaf extract with chitosan X. gideon (LEChXg) to reduce the oxidative stress of fibroblasts. The research used the human dermal fibroblast (HDF) cell line, comprising negative and positive controls and use of LEChXg 100, 200, 300, 400, and 500 µg/mL. HDF cell migration was evaluated by employing the scratch wound healing method and the wound closure was oberseved at 0, 2, 4, 6, and 24 h intervals. The cell proliferation was observed at 24, 48, and 72 h using CCK‐8 at a 450 nm wavelength. The results showed that the observations at 0, 2, and 4 h did not demonstrate any significant difference on the cell migration (p > 0.05) among the groups. However, the wound closure at 4 and 6 h showed a significant difference (p < 0.05) with LEChXg 300 µg/mL. Despite the lack of any significant variation observed up to 24 h, fibroblast subjected to the stressor did not achieve complete closure. The groups treated with LEChXg were more stable in maintaining fibroblast proliferation up to the end of the observation than those with stressors at 24, 48, and 72 h. Fibroblast induced with a stressor was also more stable in maintaining migration and proliferation in groups receiving LEChXg 300 µg/mL.

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Microencapsulation of Lemongrass Leaves Effect on Reactive Oxygen Species (ROS) Fibroblasts

Cited by 3 publications

References 37 publications

The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress

The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress

Penurunan Produksi Reactive Oxygen Species (ROS) Fibroblas dengan Nano Kitosan Kumbang Tanduk (Xylotrupes gideon)

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

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