Microencapsulation of probiotic bacteria <i>Lactobacillus plantarum</i>
 15HN using alginate-psyllium-fenugreek polymeric blends

Haghshenas, Babak; Abdullah, Norhani; Nami, Yousef; Radiah, Dayang; Rosli, Rozita; Khosroushahi, Ahmad Yari

doi:10.1111/jam.12762

Cited by 72 publications

(62 citation statements)

References 22 publications

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“…The loading abilities in Al and Ch were 16% and 20%, respectively, and these values were not significantly different (Table ). In previous studies, the EEs were approximately 92% (Praepanitchai, Noomhorm, & Anal, ) or 99% (Haghshenas et al., ) and the loading ability was approximately 14% for calcium alginate capsules (Li et al., ).…”

Section: Resultsmentioning

confidence: 91%

“…The encapsulation efficiency (EE) of the probiotics was calculated using the following expression (Haghshenas et al., ): EE (%) = ( Xt / Xi ) ×100, where Xt is the viability of JW15 in the capsules after encapsulation and Xi is the viability of JW15 (10 9 cells/mL) added during the preparation process. Cell viability was identified using a bacterial viability assay kit, following the manufacturer's protocol (ab189818; Abcam, Cambridge, UK; positive control, live‐free cells; negative control, 70% isopropanol‐treated dead cells).…”

Section: Methodsmentioning

confidence: 99%

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Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system

Kim

Nam

et al. 2020

Journal of Food Science

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The delivery of active probiotic cells in capsules can reduce probiotic cell loss induced by detrimental external factors during digestion. In this study, we determined the optimal conditions for the encapsulation of Weissella cibaria JW15 (JW15) within calcium and polyethylene glycol (PEG)-alginate with chicory root extract powder (CREP). JW15 was encapsulated as the core material (10 9 cells/mL, 2 mL/min), and a solution containing a mixture of 1.5% sodium alginate and 1% CREP was extruded into a receiving bath with 0.1 M calcium chloride (CaCl 2 ) and 0.05% PEG. Capsule morphology and size were measured using optical microscopy. The optimal air pressure and frequency vibration for capsules containing alginate only (Al) were 200 mbar and 200 Hz, respectively and 100 mbar and 350 Hz for capsules containing alginate with CREP (Ch), respectively. The voltage for both capsules types was fixed at 1.35 kV. Then, the capsules were incubated in a simulated gastrointestinal (GI) system for 6 hr at 37°C. The addition of PEG in a CaCl 2 hardening solution led to degradation of the Ch capsule (Ch-PEG) and the release of cells into the small intestine vessel in the simulated GI system. By contrast, the cells were trapped within the Al capsules. Based on these data, effective encapsulation using alginate with CREP and PEG can enable JW15 to be released at a targeted anatomical site of activity within the GI system, thereby, enhancing the efficacy of probiotic cells. These protective effects can be leveraged during the development of probiotic products.Practical Application: Weissella cibaria JW15 (10 9 cells/mL) was encapsulated in biodegradable and biocompatible capsules, prepared by mixing 1.5% alginate with 1% chicory root extract powder (CREP) in 0.1 M CaCl 2 and 0.05% PEG using an encapsulator. The optimal processing parameters were as follows: pressure, 100 mbar; vibration frequency, 350 Hz; voltage, 1.35 kV; and core flow rate, 2 mL/min. When the resulting capsules were subjected to a simulated gastrointestinal system for 6 hr, the cells were released into the small intestine, and up to 95% cell viability was preserved. These results suggest that capsules made from alginate with CREP and formulated using calcium and PEG are a promising delivery system for probiotic cells. Further reproduction without permission is prohibitedThe encapsulation efficiency (EE) of the probiotics was calculated using the following expression (Haghshenas et al., 2015): EE (%) = (Xt/Xi) ×100, where Xt is the viability of JW15 in

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Section: Resultsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system

Kim

Nam

et al. 2020

Journal of Food Science

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“…First, they may be designed to form a physical barrier that protects the probiotics from any problematic components in the surrounding environment such as gastric acids, bile salts, or digestive enzymes. Second, they may be designed to coencapsulate the probiotics with specific nutrients that help the probiotics to survive such as digestible carbohydrates, dietary fibers, proteins, lipids, or minerals (Cotter & Hill, ; Gonzalez‐Ferrero et al., ; Haghshenas et al., ; Huq et al., ; Li et al., ; Pankasemsuk, Apichartsrangkoon, Worametrachanon, & Techarang, ). Third, they may be designed to contain additives that provide a favorable local climate for the probiotics such as antacids to control the local pH (Li et al., ; Yao et al., ).…”

Section: Microencapsulation Of Probioticsmentioning

confidence: 99%

Progress in microencapsulation of probiotics: A review

Yao

Xie

et al. 2020

Comp Rev Food Sci Food Safe

325

160

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The potential health benefits of probiotics may not be realized because of the substantial reduction in their viability during food storage and gastrointestinal transit. Microencapsulation can be used to enhance the resistance of probiotics to unfavorable conditions. A range of oral delivery systems has been developed to increase the level of probiotics reaching the colon including embedding and coating systems. This review introduces emerging strategies for the microencapsulation of probiotics and highlights the key mechanisms of their stress–tolerance properties. Recent in vitro and in vivo models for evaluation of the efficiency of probiotic delivery systems are also reviewed. Encapsulation technologies are required to maintain the viability of probiotics during storage and within the human gut so as to increase their ability to colonize the colon. These technologies work by protecting the probiotics from harsh environmental conditions, as well as increasing their mucoadhesive properties. Typically, the probiotics are either embedded inside or coated with food‐grade materials such as biopolymers or lipids. In some cases, additional components may be coencapsulated to enhance their viability such as nutrients or protective agents. The importance of having suitable in vitro and in vivo models to evaluate the efficiency of probiotic delivery systems is also emphasized.

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“…Fenugreek, which contains diosgenin, may be useful for the management of diabetes-related hepatic dyslipidemias. Fenugreek might offer effective added advantages as prebiotic towards the enhancement of probiotic bacterial growth in the gastrointestinal environment [58] and is promising for development treatments on immune-related disorder [59].…”

Section: Reliable Obesity Animal Modelsmentioning

confidence: 99%

“…Fenugreek can be considered a potentially effective prebiotic for a number of beneficial applications and advances in development of treatments of immune-related disorders [58,59]. Dysbiosis of the gut microbiota is involved in severe conditions such as chronic kidney disease but whether the gut-microbiome link is altered in hypertension has not been elucidated [83].…”

Section: Preventive Medical Approachmentioning

confidence: 99%

Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

et al. 2017

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Background Worldwide obesity spread is a global health problem and needs to be further studied. Co-morbidities of obesity include insulin resistance, diabetes mellitus type 2, and dyslipidemia, which are the most frequent contributing factors for metabolic syndrome (MetS), as well as nonalcoholic fatty liver disease and chronic kidney disease. The aim was to study renal function and endogenous intoxication panel on high-calorie diet-induced obesity rat model and perform comparative study of the treatment efficacy of Fenugreek-based bionanocomposite vs antiobesogenic drugs (Orlistat). Materials We included 60 male rats and equally divided them to 6 groups of 10 animals in each group: the experimental groups were firstly assigned as controls and high caloric diet (HCD)-fed groups, and each group further was subdivided to remain untreated, Fenugreek bionanocomposite (BNC)-treated, and Orlistat-treated. Normal control rats (groups 1, 2, 3) were fed by a standard chow, while the others (groups 4, 5, 6) were fed with HCD ad libitum during 98 days. From days 77 to 98, groups 2 and 5 were treated with BNC based on Fenugreek (150 mg/kg body weight, orally) and groups 3 and 6 were treated with antiobesogenic drug Orlistat (10 mg/kg body weight, orally). Food and water consumptions were measured daily and body weights were measured once a week. On day 99, blood was collected; the creatinine, urea, and uric acid were estimated in serum according to the standard protocols. Levels of low and middle molecules (MMs) were measured; the quantity of oligopeptides was estimated by Bradford method. We performed the liver and kidney ultrasonography in rats.Results We revealed an increase in the levels of endogenous intoxication syndrome markers (MM and oligopeptides) in all animals with experimental obesity. Ultrasound data showed injury of the liver and kidneys in obese rats. We observed significant decreasing of MM levels after Orlistat treatment vs controls (p < 0.05). However, this effect was more pronounced in Fenugreek BNC-treated group vs both Orlistattreated and controls (p < 0.05). Orlistat treatment evoked rising of serum creatinine and oligopeptides in control animals

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Microencapsulation of probiotic bacteria Lactobacillus plantarum 15HN using alginate-psyllium-fenugreek polymeric blends

Abstract: Herbal-based biopolymers offer added advantages of being prebiotic towards the enhancement of probiotic bacterial growth in the gastrointestinal environment.

Cited by 72 publications

References 22 publications

Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system

Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system

Progress in microencapsulation of probiotics: A review

Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

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