Microfluidic paper analytical device for the chromatographic separation of ascorbic acid and dopamine

Murphy, Alan; Gorey, Brian; Guzman, Keana De; Kelly, N.; Nesterenko, Ekaterina P.; Morrin, Aoife

doi:10.1039/c5ra16272f

Cited by 32 publications

(11 citation statements)

References 24 publications

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“…Our results show that the approach presented here can be used to develop lateral flow tests with electrochemical detection easily and without the need for sophisticated screen-printing equipment. [36][37][38] Although the same technique could be applied for making adhesive stencils and transfer tapes, 17,39 the presence of an adhesive layer facilitates the integration of lateral flow membranes in the devices. In fact, the pressure sensitive adhesive used in this work is customarily used as a backing material in conventional lateral flow applications.…”

Section: Discussionmentioning

confidence: 99%

Rapid prototyping of electrochemical lateral flow devices: stencilled electrodes

Pellitero

Κιτσαρά

Eibensteiner³

et al. 2016

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A straightforward and very cost effective method is proposed to prototype electrodes using pressure sensitive adhesives (PSA) and a simple cutting technique. Two cutting methods, namely blade cutting and CO2 laser ablation, are compared and their respective merits are discussed. The proposed method consists of turning the protective liner on the adhesive into a stencil to apply screen-printing pastes. After the electrodes have been printed, the liner is removed and the PSA can be used as a backing material for standard lateral flow membranes. We present the fabrication of band electrodes down to 250 μm wide, and their characterization using microscopy techniques and cyclic voltammetry. The prototyping approach presented here facilitates the development of new electrochemical devices even if very limited fabrication resources are available. Here we demonstrate the fabrication of a simple lateral-flow device capable of determining glucose in blood. The prototyping approach presented here is highly suitable for the development of novel electroanalytical tools.

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Section: Discussionmentioning

confidence: 99%

Rapid prototyping of electrochemical lateral flow devices: stencilled electrodes

Pellitero

Κιτσαρά

Eibensteiner³

et al. 2016

Analyst

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“…The surface of paper will acquire a relatively low cationexchange capacity and can retain metal cations through the electrostatic interactions. 29,30 Therefore, a portion of the analyte can be adsorbed on the surface of the paper normally available at the sample zone of other μPADs without even reaching to the detection area. This issue will be more considerable when very low concentrations of the metals are being monitored.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Even though paper is conventionally considered as a neutral material, but the overall surface charge of the cellulose is negative due to the presence of the carboxyl groups formed by the addition of oxidative reagents to the fibers during papermaking process. The surface of paper will acquire a relatively low cation-exchange capacity and can retain metal cations through the electrostatic interactions. , Therefore, a portion of the analyte can be adsorbed on the surface of the paper normally available at the sample zone of other μPADs without even reaching to the detection area. This issue will be more considerable when very low concentrations of the metals are being monitored.…”

Section: Resultsmentioning

confidence: 99%

Geometrical Alignment of Multiple Fabrication Steps for Rapid Prototyping of Microfluidic Paper-Based Analytical Devices

et al. 2017

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Three main fabrication steps for microfluidic paper-based analytical devices (μPADs) were fully integrated with accurate geometrical alignment between the individual steps in a simple and rapid manner. A wax printer for creating hydrophobic barriers was integrated with an inexpensive (ca. $300) electronic craft plotter/cutter for paper cutting, followed by colorimetric reagent deposition using technical pens. The principal shortcoming in the lack of accurate and precise alignment of the features created by these three individual fabrication steps was addressed in this work by developing appropriate alignment procedures during the multistep fabrication process. The wax printing step was geometrically aligned with the following cutting and plotting (deposition) steps in a highly accurate and precise manner using optical scanning function of the plotter/cutter based on registration marks printed on the paper using the wax printer and scanned by the plotter/cutter. The accuracy and precision of alignment in a two-dimensional plane were quantified by cutting and plotting cross-shaped features and measuring their center coordinates relative to wax printed reference lines. The average accuracy along the X- and Y-axis was 0.12 and 0.16 mm for cutting and 0.19 and 0.17 mm for plotting, respectively. The potential of this approach was demonstrated by fabricating μPADs for instrument-free determination of cobalt in waters using distance-based readout, with excellent precision (%RSD = 5.7) and detection limit (LOD) of 2.5 ng and 0.5 mg/L (mass and concentration LODs, respectively).

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“…While μPADs have traditionally been used for assays, there has been a recent effort to adapt separation methods based on decades old paper chromatography and electrophoresis methods − where μPADs can add new functionality. Strategies for manipulating fluids in μPADs include designing geometry with varying channel length, width, and shape, changing chemical properties in the flow path to adjust flow rate, , or using mechanical means to maintain or stop the fluid flow. Electrophoresis on paper has been of interest for separation and subsequent detection owing to paper’s inherent advantages such as hydrophilicity and self-pumping.…”

Section: Other Technologiesmentioning

confidence: 99%

Paper-Based Microfluidic Devices: Emerging Themes and Applications

et al. 2016

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Microfluidic paper analytical device for the chromatographic separation of ascorbic acid and dopamine

Abstract: Cellulose-based filter papers were used as base materials to construct microfluidic paper-based analytical devices (μPADs) coupling a separation channel with electrochemical detection.

Cited by 32 publications

References 24 publications

Rapid prototyping of electrochemical lateral flow devices: stencilled electrodes

Rapid prototyping of electrochemical lateral flow devices: stencilled electrodes

Geometrical Alignment of Multiple Fabrication Steps for Rapid Prototyping of Microfluidic Paper-Based Analytical Devices

Paper-Based Microfluidic Devices: Emerging Themes and Applications

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