Microfluidics-based extraction of viral RNA from infected mammalian cells for disposable molecular diagnostics

Polymeric-Based In Vitro Diagnostic Devices

“…Capture and purification of RNA using silica beads has been accomplished for E. coli RNA and viral RNA from influenza-A (H1N1)-infected mammalian cells [209] with real-time nucleic acid sequence-based amplification (NASBA) for target sequence amplification [210]. A combination of chemical and mechanical hybrid methods for bacterial cells lysis has also been reported [211].…”

Review on recent and advanced applications of monoliths and related porous polymer gels in micro-fluidic devices

“…On-chip end-point fluorescence detection was carried out by means of an external LED excitation source and a spectrophotometer. Theoretical detection limits were in the order of 1 molecule in 50 L. Previous versions of this device containing the same monolithic SPE column, but no on-chip PCR, were also used to isolate nucleic acids from gram positive and negative bacteria [93,94] and viruses [95] in whole blood [94], urine [93] and cell culture [95] samples.…”

Impact of microfluidic processing on bacterial ribonucleic acid expression

“…2 However, these systems performed only the detection stage of the on-chip process. Microfluidics has also been utilised for on-chip extraction of viral RNA in blood 12 and for bacterial RNA extraction, 13 but the RNA expression was not benchmarked against traditional processes. A major challenge in using RNA expression lies in processing and extracting, in a robust and repeatable manner, RNA from a patient sample before the occurrence of any changes in RNA expression.…”