Micrografting of mature stone pine (<i>Pinus pinea </i>L.) trees

Cortizo, Millán; Alonso, P. Vega; Fernández, Belén; Rodrı́guez, Ana; Centeno, Marı́a Luz; Ordás, Ricardo J.

doi:10.1051/forest:2004081

Cited by 16 publications

(24 citation statements)

References 7 publications

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“…The decrease in vigor and adventitious organs formation capacity are responses associated with the tissue maturation stage [4]. Hence, basal scions must present higher morphogenic capacities than apical ones, because their younger ontogenetic age determined by the proximity to the mother cell located at the collar of the ortet [5] [6]. In the consolidation phase we found that juvenile traits of ontogenically young tissues can be preserved in basal scions.…”

Section: Resultsmentioning

confidence: 61%

“…This higher photochemical activity is a proxy of higher CO 2 assimilation capacity, and therefore of greater photosynthetic performance and potentially faster growth [18] [20] [21]. Additionally higher Fv/Fm reflects an ontogenetic age gradient of shoot tips morphogenic competence, where basal meristematic shoot tips present higher morphogenic capacities than apical ones [5] [6]. Finally, based on our results, we conclude that Fv/Fm can be an indicator of the micrograft's development according to the shoot tips position in the ortet and can be a useful indicator of the physiological stage of scions during micrograft transition from establishment to consolidation.…”

Section: Resultsmentioning

confidence: 99%

“…Commonly, the development of shoot tips and scion meristematic activity expressed as growth rates are key factors that determine micrograft success [3] [5]. The decrease in vigor and adventitious organs formation capacity are responses associated with the tissue maturation stage [4].…”

Section: Resultsmentioning

confidence: 99%

“…Several studies conducted in Pinus sp. indicate an ontogenetic gradient of shoot tips morphogenic competence, determined by the vertical localization in the tree crown, where basal meristematic shoot tips present higher morphogenic capacities than apical ones [5]- [7]. It has been suggested that the decline in morphogenic capacity could be due to the loss in competence at the cellular level and it is highly likely that this phenomenon causes changes in gene transcription [8].…”

Section: Introductionmentioning

confidence: 99%

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Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

Materán¹,

Sáez²,

Sánchez‐Olate³

et al. 2015

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How to cite this paper: Materán, M.E., et al. (2015) M. E. Materán et al. 229lower Fv/Fm than apical shoot tips, which agrees with the lowest viability (35%). However, during the consolidation phase, the trend changed and basal shoot tips presented higher Fv/Fm than apical shoot tips and showed an increase in ETR and NPQ, with respect to apical shoots and ortet. Although the measurement of fluorescence parameters implies the insertion of the fluorometer sonde in vitro, this implies aseptic considerations, but always conveies a contamination risk. We conclude that fluorescence (Fv/Fm, ETR, NPQ) can be indicators of the micrograft's development according to the shoot tips position in the ortet and can be useful early-indicators of the scions' physiological condition during micrograft transition from establishment to consolidation.

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Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photochemical Efficiency during the Establishment and Consolidation Phases of in Vitro Pinus radiata Micrograft Made from Scions of Different Ontogenetic Age

Materán¹,

Sáez²,

Sánchez‐Olate³

et al. 2015

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“…Antepfıstığının Siirt çeşidiyle ilgili ana bitkilerden alınma zamanlarına göre elde edilen başarının Nisan %70, Haziran %80 olduğu, bunun aksine Şubat ayı içerisinde alınan eksplantlardaki aşı başarısının %10 olduğu bildirilmiştir (Onay ve ark., 2004). Fıstık çamı ve monteri çamı bitkilerde yapılan aşılamalarda mevsimsel değişim açısından en iyi aşı başarısı bitkilerin kış dinlenme periyodunda alınan eksplantlarından elde edilmiştir (Fraga et al, 2002;Cortizo et al, 2004). Dinlenme halindeki sürgünlerde daha iyi aşı başarısı sağlanan türler olmakla birlikte; bunun aksine aktif gelişme döneminde daha iyi sonuç veren türlerde bulunmaktadır.…”

Section: Fizyolojik Koşullarunclassified

In vitro Micrografting of Woody Plant Species-I (Rootstock, Scion, Grafting Technique)

Çalar¹,

Onay²,

Karlıdağ³

et al. 2017

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ÖZET: İn-vitro aşılama, aksenik kültür koşullarında minyatür aşı kalemlerinin aşılanmasını kapsayan ve diğer tekniklere nazaran en son vejetatif çoğaltım tekniklerinden biridir. Bu yöntem, sürgün ucu kültürü ve aşılamanın bazı sınırlayıcı özelliklerinin üstesinden gelmekle birlikte, her iki metodun avantajlarını da bir arada bulundurmaktadır. İlk uygulandığı zamanlarda bazı meyve tür ve çeşitlerindeki virüs ve benzeri endojen patojenlerin eradikasyonu için geliştirilen in-vitro aşılama, bitki gelişim ve fizyolojisinin farklı alanlarında çeşitli odunsu bitki türlerinde hızla gelişmiştir. Bunlar birçok odunsu türlerin olgun genotiplerinin klonal çoğaltımında bir ön koşul olarak fizyolojik rejuvenasyonu ve aşıda uyuşmazlığı da kapsamaktadır. Sonuç olarak in-vitro aşılama, yoğun olarak kullanılan ve diğer vejatatif çoğaltım metodlarında bulunan olumsuzlukların üstesinden gelebilmek için daha çok düşünülmeyi ve kullanılmayı hak eden orijinal ve tecrübe gerektiren bir tekniktir. Doku ve hücreler arasındaki genetik benzerlik ve farklılıkların ayrıntılı incelenmesine imkan sağlamaktadır. Bu çalışmanın amacı, 1970'li yıllarda turunçgillerde virüsten ari bitki üretimi amacıyla başlayan in vitro aşılama çalışmalarının günümüze kadar nasıl bir süreçten geçtiği ve özellikle meyve türlerinin de dahil olduğu odunsu bitki türlerinde bitki ıslahı ve çoğaltımı amacıyla ne tür çalışmaların yapıldığının ortaya çıkarılması ve belli bir düzende sunulmasıdır.Anahtar Kelimeler: Aşılama, in-vitro, kalem ABSTRACT: In vitro micrografting is a propagation technique, involving the grafting of relatively miniature cuttings under axenic culture conditions and it is one of the recent developed propagation techniques compared to other conventional vegetative propagation techniques. This method overcomes some of the limitations of shoot tip culture and grafting, while it also keeps together the advantages of both methods. Micrografting was applied for the eradication viruses and pathogens from some fruit species and cultivars during the first application period, but later, the technique was further developed on various woody plant species in different research areas of plant physiology and development. These includes physiological rejuvenation and incompatibility grafting as a prerequisite for the clonally propagation of mature genotypes of many woody species. Consequently, in vitro micrografting is used in large scale propagation and an original technique which needs experience by overcoming the disadvantages of other propagation technique. It also enables to examine in detail the genetic similarities and differences between the tissues and cells. The aims of this study were (1) to review how micrografting studies passed a process from 1970s until today, first started to obtain virus-free plants from citrus; (2) to reveal what kind of work has been presented particularly on the plant breeding and propagation of the woody plant species, including the type of fruit breeding and reproduction and (3) and to present those studies in a specific order.

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