Microgravimetric lectin biosensor based on signal amplification using carbohydrate-stabilized gold nanoparticles

Abstract

A highly sensitive microgravimetric lectin biosensor has been developed using carbohydrate-stabilized Au nanoparticles as a signal amplifier; mannose-stabilized Au nanoparticles formed a sandwich-type complex with the target Con A specifically bound to a mannose-modified Au QCM electrode to give an amplified frequency response.

Cyclic voltammetric studies of carbohydrate–protein interactions on gold surface

“…Surface-based analytical techniques such as SPR and QCM have been used to study carbohydrate-lectin interactions since the carbohydrate self-assembled monolayers (SAMs) on electrode surface can closely resemble cell surface. Thus, these QCM and SPR analysis coupled with carbohydrate SAMs can facilitate to understand the carbohydrate-lectin interactions in biological systems through controlling the density and orientation of the carbohydrate SAMs on surface [14,15,[18][19][20][21][22]. While these SPR and QCM analysis are real-time and labelfree, these methods demand complicated instrumentation and technical expertise.…”

“…Up to date, various analytical methods have been developed to study the carbohydrate-lectin interactions, which include isothermal calorimetry (ITC) [9][10][11], frontal affinity chromatography (FAC) [12], nuclear magnetic resonance (NMR) spectroscopy [13], surface plasmon resonance (SPR) [14][15][16][17][18], and quartz crystal microbalance (QCM) [18][19][20][21]. Although solution-based analytical techniques such as ITC, FAC, and NMR are known to be effective to study the multivalent carbohydrate-lectin interactions, they often require large amount of materials for analysis.…”

Carbohydrate derivative-functionalized biosensing toward highly sensitive electrochemical detection of cell surface glycan expression as cancer biomarker

“…Consequently, many kinds of biosensors were developed for glycan analysis based on optical, electrochemical, and piezoelectric transducers (Park et al, 2009;Dai et al, 2006;Lyu et al, 2008;Svarovsky and Joshi, 2014). Among them, electrochemical biosensors have been well recognized to be a promising solution for signal transduction due to the fact that electrochemical detectors are simple, portable and inexpensive Peng et al, 2014;Zheng et al, 2014).…”

The Application of Biomolecules in the Preparation of Nanomaterials

“…These amino-dextran protected gold and silver nanoparticles can be used as biosensor for the detection of concanavalin A. The second method utilizes the sugar, such as mannose and dextran, to modify the asprepared nanoparticles Aslan et al, 2004;Lyu et al, 2008). These sugar modified nanoparticles can be used as biosensor for the detection of concanavalin A and glucose.…”