Microimpedance tomography system to monitor cell activity and membrane movements in a breathing lung-on-chip

Mermoud, Yves; Felder, Marcel; Stucki, Janick; Stucki, Andreas; Guénat, O.

doi:10.1016/j.snb.2017.09.192

Cited by 71 publications

(53 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the perspective given by these preliminary findings appear stimulating, showing the possibility to correlate quantitative changes of impedance magnitude and phase with cell growth in the scaffold. These approaches might furthermore be combined with the most upcoming strategies in term of impedance-based assays (e.g., impedance tomography) [122]. Integrating the two approaches might give a significant contribute in non-invasive monitoring of 3D cell culture with a significant improvement for field like regenerative medicine and drug development.…”

Section: Discussionmentioning

confidence: 99%

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

et al. 2019

View full text Add to dashboard Cite

During the last years, scientific research in biotechnology has been reporting a considerable boost forward due to many advances marked in different technological areas. Researchers working in the field of regenerative medicine, mechanobiology and pharmacology have been constantly looking for non-invasive methods able to track tissue development, monitor biological processes and check effectiveness in treatments. The possibility to control cell cultures and quantify their products represents indeed one of the most promising and exciting hurdles. In this perspective, the use of conductive materials able to map cell activity in a three-dimensional environment represents the most interesting approach. The greatest potential of this strategy relies on the possibility to correlate measurable changes in electrical parameters with specific cell cycle events, without affecting their maturation process and considering a physiological-like setting. Up to now, several conductive materials has been identified and validated as possible solutions in scaffold development, but still few works have stressed the possibility to use conductive scaffolds for non-invasive electrical cell monitoring. In this picture, the main objective of this review was to define the state-of-the-art concerning conductive biomaterials to provide researchers with practical guidelines for developing specific applications addressing cell growth and differentiation monitoring. Therefore, a comprehensive review of all the available conductive biomaterials (polymers, carbon-based, and metals) was given in terms of their main electric characteristics and range of applications.

show abstract

Section: Discussionmentioning

confidence: 99%

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

et al. 2019

View full text Add to dashboard Cite

show abstract

“…After that, a cyclic mechanical stretch is applied to the PDMS membrane (21%, 2D, at frequency of 0.2 Hz) by exerting a negative pressure. The modified version of this model enables online monitoring of transepithelial electrical resistance (TEER) in real‐time using a microimpedance tomography system (at a distance of 1 mm from the electrodes) . In addition, the microfluidic device used in this study can be equipped with a passive medium exchange unit to provide long‐term culture conditions under ALI …”

Section: Stretch Devices For In Vitro Cell Models Of the Lungmentioning

confidence: 99%

Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Doryab

Taş

Taskin

et al. 2019

Adv Funct Materials

View full text Add to dashboard Cite

Mechanical stretch under both physiological (breathing) and pathophysiological (ventilator-induced) conditions is known to significantly impact all cellular compartments in the lung, thereby playing a pivotal role in lung growth, regeneration and disease development. In order to evaluate the impact of mechanical forces on the cellular level, in vitro models using lung cells on stretchable membranes have been developed. Only recently have some of these cell-stretching devices become suitable for air-liquid interface cell cultures, which is required to adequately model physiological conditions for the alveolar epithelium. To reach this goal, a multi-functional membrane for cell growth balancing biophysical and mechanical properties is critical to mimic (patho)physiological conditions. In this review, i) the relevance of cyclic mechanical forces in lung biology is elucidated, ii) the physiological range for the key parameters of tissue stretch in the lung is described, and iii) the currently available in vitro cell-stretching devices are discussed. After assessing various polymers, it is concluded that natural-synthetic copolymers are promising candidates for suitable stretchable membranes used in cell-stretching models. This work provides guidance on future developments in biomimetic in vitro models of the lung with the potential to function as a template for other organ models (e.g., skin, vessels).

show abstract

“…IgG 1 had the preferential transfer, while IgG 2 had the slowest transfer [100] evaluated the differences of placental elasticity between normal and IUGR pregnancies using perfusion model IUGR group displayed more histopathological changes and were stiffer than the control group, and that reduced placental elasticity could be the cause of these differences [101] examined the maternal transfer of interleukin-6 in the ex vivo perfusion model minimal maternal to fetal transfer of interleukin-6 was observed from the ex vivo perfusion model [102] royalsocietypublishing.org/journal/rsfs Interface Focus 9: 20190031 growth factors, cytokines, hormones, nutrients, intercellular junctions and extracellular matrices are all additional examples of soluble and insoluble aspects that can be mimicked through the use of a microengineered co-culture model of a placenta-on-a-chip [72]. Data and tests have also proven well for microfluidic devices, and have opened the doors to a realm of organson-chips [120]: bone-on-a-chip [121], liver-on-a-chip [122], body-on-a-chip [123], kidney-on-a-chip [124], lung-on-a-chip [125], cancer-on-a-chip [126], heart-on-a-chip [127] and brain-on-a-chip [128]. The following organs-on-chips reveal that the on-a-chip process is used quite frequently in the medical industry, and is becoming ever so demanding for the better understanding of the human body.…”

Section: Microfluidic Devicesmentioning

confidence: 99%

Drug transport across the human placenta: review of placenta-on-a-chip and previous approaches

2019

View full text Add to dashboard Cite

One contribution of 15 to a theme issue 'Bioengineering in women's health, volume 2: pregnancy-from implantation to parturition'.In the past few decades, the placenta became a very controversial topic that has had many researchers and pharmacists discussing the significance of the effects of pharmaceutical drug intake and how it is a possible leading cause towards birth defects. The creation of an in vitro microengineered model of the placenta can be used to replicate the interactions between the mother and fetus, specifically pharmaceutical drug intake reactions. As the field of nanotechnology significantly continues growing, nanotechnology will become more apparent in the study of medicine and other scientific disciplines, specifically microengineering applications. This review is based on past and current research that compares the feasibility and testing of the placenta-on-a-chip microengineered model to the previous and underdeveloped in vivo and ex vivo approaches. The testing of the practicality and effectiveness of the in vitro, in vivo and ex vivo models requires the experimentation of prominent pharmaceutical drugs that most mothers consume during pregnancy. In this case, these drugs need to be studied and tested more often. However, there are challenges associated with the in vitro, in vivo and ex vivo processes when developing a practical placental model, which are discussed in further detail.

show abstract

Microimpedance tomography system to monitor cell activity and membrane movements in a breathing lung-on-chip

Cited by 71 publications

References 20 publications

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Drug transport across the human placenta: review of placenta-on-a-chip and previous approaches

Contact Info

Product

Resources

About