Micronucleus assay in bovine lymphocytes after exposure to bisphenol A in vitro

Šutiaková, Irena; Kovalkovičová, Natália; Šutiak, V.

doi:10.1007/s11626-013-9727-9

Cited by 10 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22,23,24,26 Here, we discuss data obtained from our in vitro study conducted in order to establish the clastogenic and aneugenic potential of BP-A on human cultured lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The genotoxicity of BP-A has been evaluated in several in vitro and in vivo studies, although with controversial results. [22][23][24]26 Here, we discuss data obtained from our in vitro study conducted in order to establish the clastogenic and aneugenic potential of BP-A on human cultured lymphocytes. The results obtained in the present in vitro study with CAs and MNs assays evidenced a possible clastogenic effect of the BP-A on human lymphocytes, also at the concentration of 0.05 mg/mL that represents the RD established for humans by the US EPA.…”

Section: Discussionmentioning

confidence: 99%

“…BP-A was found to be able to induce increased chromosome aberrations (CAs) and DNA adducts in Syrian hamster embryo cells, 19 micronuclei (MNs) in human MCL-5 cells 20 and in fish erythrocytes, 21 MNs and CAs formation in chines hamster ovary (CHO) cells. 22 A significant genotoxic effect by BP-A was also seen on bovine peripheral lymphocytes in vitro, but only at the highest concentration of 1 × 10 −4 M. 23 Moreover, Tiwari et al 24 observed a significant increase of MNs in polychromatic erythrocytes, structural CAs in bone marrow cells and DNA damage in lymphocytes of rats exposed to BP-A. Interestingly, in HEK293 cells, BP-A was found to alter the expression of some genes involved in important biological processes including ion transport, cysteine and glycogen metabolic processes, apoptosis, DNA damage repair and so on.…”

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Clastogenic effects of bisphenol A on human cultured lymphocytes

et al. 2017

View full text Add to dashboard Cite

Abstract:Bisphenol-A is an endocrine disrupting compound widely used in the production of polycarbonate plastics and epoxy resins. It is ubiquitously present in the environment, mostly in aquatic environments, with consequent risks to the health of aquatic organisms and humans. In the present study we analysed the cytogenetic effects of bisphenol-A on human lymphocytes using in vitro chromosomal aberrations and micronuclei assays. Lymphocyte cultures were exposed to five different concentrations of BP-A (0.20, 0.10, 0.05, 0.02 and 0.01 µg/mL) for 24 h (for chromosomal aberrations test) and 48 h (for micronuclei test). The concentration of 0.05 µg/mL represents the reference dose established by United States Environmental Protection Agency; 0.02 µg/mL represents the higher concentration of unconjugated BP-A found in human serum and 0.01 µg/mL represents the tolerable daily intake established by European Union. Data obtained with both assays showed significant genotoxic effects of the bisphenol-A at concentrations of 0.20, 0.10 and 0.05 µg/mL, whereas at the concentration of 0.02 µg/mL we observed only a significant increase in the micronuclei frequency. Finally, at the concentration of 0.01 µg/mL no cytogenetic effects were observed, indicating this latter as a more tolerable concentration for human health with respect to 0.05 µg/mL, the reference dose established by US EPA.

show abstract

“…22,23,24,26 Here, we discuss data obtained from our in vitro study conducted in order to establish the clastogenic and aneugenic potential of BP-A on human cultured lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Clastogenic effects of bisphenol A on human cultured lymphocytes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In one of these (Santovito et al, 2018 [RefID 11220]), MN frequency was also measured. A study of MN in bovine peripheral blood lymphocytes also reported positive findings (Šutiaková et al, 2014 [RefID 7026]).…”

Section: Assessmentmentioning

confidence: 90%

Re‐evaluation of the risks to public health related to the presence of bisphenol A (BPA) in foodstuffs

Lambré¹,

Baviera²,

Bolognesi³

et al. 2023

EFS2

View full text Add to dashboard Cite

In 2015, EFSA established a temporary tolerable daily intake (t‐TDI) for BPA of 4 μg/kg body weight (bw) per day. In 2016, the European Commission mandated EFSA to re‐evaluate the risks to public health from the presence of BPA in foodstuffs and to establish a tolerable daily intake (TDI). For this re‐evaluation, a pre‐established protocol was used that had undergone public consultation. The CEP Panel concluded that it is Unlikely to Very Unlikely that BPA presents a genotoxic hazard through a direct mechanism. Taking into consideration the evidence from animal data and support from human observational studies, the immune system was identified as most sensitive to BPA exposure. An effect on Th17 cells in mice was identified as the critical effect; these cells are pivotal in cellular immune mechanisms and involved in the development of inflammatory conditions, including autoimmunity and lung inflammation. A reference point (RP) of 8.2 ng/kg bw per day, expressed as human equivalent dose, was identified for the critical effect. Uncertainty analysis assessed a probability of 57–73% that the lowest estimated Benchmark Dose (BMD) for other health effects was below the RP based on Th17 cells. In view of this, the CEP Panel judged that an additional uncertainty factor (UF) of 2 was needed for establishing the TDI. Applying an overall UF of 50 to the RP, a TDI of 0.2 ng BPA/kg bw per day was established. Comparison of this TDI with the dietary exposure estimates from the 2015 EFSA opinion showed that both the mean and the 95th percentile dietary exposures in all age groups exceeded the TDI by two to three orders of magnitude. Even considering the uncertainty in the exposure assessment, the exceedance being so large, the CEP Panel concluded that there is a health concern from dietary BPA exposure.

show abstract

“…In a previous study, lymphocytes were isolated from the peripheral blood of bovines, and the genotoxic effect of BPA was determined at different concentrations, 1 Â 10 À4 , 1 Â 10 À5 , 1 Â 10 À6 , and 1 Â 10 À7 mol/L. And it was found that nuclear divisions for cell proliferation did not change with BPA application, but the number of micronuclei was enhanced with increasing concentrations of BPA (Sutiaková et al, 2014). In our study, we did not examine cell proliferation, although as a result of BPA exposure, the number of CD3 positive cells was decreased in both groups of day 21 and 45.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage

et al. 2020

View full text Add to dashboard Cite

Bisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has a steroid hormone-like activity; therefore, it poses a danger to human health. In our study, we aimed to investigate the effects of BPA on lymph node and spleen in male rats exposed to this agent during prenatal stage. The pregnant female rats were divided into four groups: control, sham, low dose (300 µg/kg BPA), and high dose (900 µg/kg BPA). BPA was dissolved in 1 mL of corn oil and administered to the pregnant rats every day during pregnancy. On the 21st and 45th day after the birth, male rats’ lymph node and spleen samples were taken and histopathological examination was performed. Samples were stained with hematoxylin and eosin to determine the general histological appearance, and with CD3 and CD20 immunohistochemically. The results of staining were evaluated by H-score, and statistical analysis was performed. In the samples, BPA applications were not found to cause significant tissue damage. But there was a significant decrease in the immunoreactivities of CD3 and CD20 after BPA applications in both 21st and 45th day samples. After high dose BPA administration, decreased CD3 immunoreactivity was statistically significant. It is thought that BPA does not cause histologically significant tissue damage, but it may impair organ function at cellular level. The investigation of molecules involved in organ function will be useful in revealing the mechanisms that will cause dysfunction.

show abstract

Micronucleus assay in bovine lymphocytes after exposure to bisphenol A in vitro

Cited by 10 publications

References 26 publications

Clastogenic effects of bisphenol A on human cultured lymphocytes

Clastogenic effects of bisphenol A on human cultured lymphocytes

Re‐evaluation of the risks to public health related to the presence of bisphenol A (BPA) in foodstuffs

Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage

Contact Info

Product

Resources

About