Micropatterning with aerosols: Application for biomaterials

Gagné, Louis; Rivera, Gerardo; Laroche, Gaétan

doi:10.1016/j.biomaterials.2006.06.006

Cited by 15 publications

(35 citation statements)

References 41 publications

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“…Consequently, the overall biofabrication process should proceed from a combination of processes and technologies interacting with bioprinting. Technologies such as fused deposition modelling [27], aerosols [28], electrospinning [29] as well as optically driven methods (such as photopoly- merizing, micromachining, laser catapulting [30], LGDW [10,11]) could thus be combined with bioprinting when the microarchitecture and the cell microenvironment of some tissue parts or layers do not require precise positioning of components. Consequently, a coefficient expressing the integrability or the interacting capacity of the bioprinting method has to be introduced in Eq.…”

Section: Discussionmentioning

confidence: 99%

High-throughput laser printing of cells and biomaterials for tissue engineering

et al. 2010

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Section: Discussionmentioning

confidence: 99%

High-throughput laser printing of cells and biomaterials for tissue engineering

et al. 2010

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“…The PTFE samples were placed 21 cm below the needle on a custom‐made stage mounted on the y ‐axis rail of an x–y computer‐controlled table (Velmex, Bloomfiel, NY). A pattern consisting of 10.1 ± 0.1 μm average diameter CGRGDS spots over a background of CWQPPRARI was obtained as previously described 36. The CGRGDS spray was generated at an air pressure of 30 psi and a flow of 1000 μL/h while displacing the PTFE surface at 7.9 mm/s over 15 cm x ‐axis distances and 0.5 cm y ‐axis increments in order to obtain 20 ± 5% surface coverage by the CGRGDS droplets.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously described two novel micropatterning techniques based on inkjet printing17 and aerosol spray deposition36 that enhance the biocompatibility of polytetrafluoroethylene (PTFE). The two peptide solutions used to generate the patterns consisted of CGRGDS to promote cell adhesion and CWQPPRARI to promote cell proliferation and migration 37–39.…”

Section: Introductionmentioning

confidence: 99%

“…The two peptide solutions used to generate the patterns consisted of CGRGDS to promote cell adhesion and CWQPPRARI to promote cell proliferation and migration 37–39. In static cultures, higher endothelial cell yields were observed on the micropatterned surfaces than on surfaces treated with a single peptide solution 17, 36. Moreover, the size and spacing of the micropatterns affected these yields.…”

Section: Introductionmentioning

confidence: 99%

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Human saphenous vein endothelial cell adhesion and expansion on micropatterned polytetrafluoroethylene

Boivin¹,

Chevallier²,

Hoesli³

et al. 2012

J Biomedical Materials Res

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Intimal hyperplasia and thrombosis are responsible for the poor patency rates of small-diameter vascular grafts. These complications could be avoided by a rapid and strong adhesion of endothelial cells to the prosthetic surfaces, which typically consist of expanded polytetrafluoroethylene (PTFE) for small-diameter vessels. We have previously described two peptide micropatterning strategies that increase the endothelialization rates of PTFE. The micropatterns were generated either by inkjet printing 300 μm squares or by spraying 10.1 ± 0.1 μm diameter droplets of the CGRGDS cell adhesion peptide, while the remaining surface was functionalized using the CWQPPRARI cell migration peptide. We now directly compare these two micropatterning strategies and examine the effect of hydrodynamic stress on human saphenous vein endothelial cells grown on the patterned surfaces. No significant differences in cell adhesion were observed between the two micropatterning methods. When compared to unpatterned surfaces treated with a uniform mixture of the two peptides, the cell expansion was significantly higher on sprayed or printed surfaces after 9 days of static cell culture. In addition, after 6 h of exposure to hydrodynamic stress, the cell retention and cell cytoskeleton reorganization on the patterned surfaces was improved when compared to untreated or random treated surfaces. These results indicate that micropatterned surfaces lead to improved rates of PTFE endothelialization with higher resistance to hydrodynamic stress.

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“…The work that sprayed ePTFE with various cell adhesive peptides (described at the end of section 2) has implications for improving the endothelialization of Gore-Tex grafts. 24 Having an endothelial layer may improve the integration and integrity of the grafts by decreasing tissue ingrowth and thrombus formation. However, the technique for spraying the peptides was developed for a flat surface and was not applied to the vessel lumen, indeed, no proposal for an appropriate way to transition this technology from flat surface to tubes was made by the authors.…”

Section: Applications For Regenerative Medicinementioning

confidence: 99%

Endothelial Cell Micropatterning: Methods, Effects, and Applications

Anderson

Hinds

2011

Ann Biomed Eng

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The effects of flow on endothelial cells have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of endothelial cell morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines endothelial cell micropatterning research by exploring both the many alternative methods used to alter endothelial cell morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in endothelial cell proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, is discussed.

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Micropatterning with aerosols: Application for biomaterials

Cited by 15 publications

References 41 publications

High-throughput laser printing of cells and biomaterials for tissue engineering

High-throughput laser printing of cells and biomaterials for tissue engineering

Human saphenous vein endothelial cell adhesion and expansion on micropatterned polytetrafluoroethylene

Endothelial Cell Micropatterning: Methods, Effects, and Applications

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