Micropropagation, encapsulation and growth of Artemisia vulgaris node explants for germplasm preservation

Sujatha, G.; Kumari, B. D. Ranjitha

doi:10.1016/j.sajb.2007.09.002

Cited by 31 publications

(11 citation statements)

References 33 publications

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“…On the other hand, the viability of encapsulated shoots stored at 25±2°C was markedly influenced by light: after 30 d of storage greater viability was observed when illumination was provided than in continuous darkness. Temperature and light requirements have been studied in different species (Micheli et al 2007;Sujatha and Ranjitha Kumari 2008), and a number of studies report that normal growth-room temperature and low illumination is beneficial for encapsulation of plant parts (Janeiro et al 1995;Divakaran et al 2006). The viability and regeneration capacity was checked every 30 d by inoculating the shoots into MS medium containing 1.11 μM BA.…”

Section: Resultsmentioning

confidence: 99%

In vitro approaches for conservation of Asparagus racemosus Willd.

Thakur

Tiwari

Jadhav

2015

In Vitro Cell.Dev.Biol.-Plant

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Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in India. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species. The plants are being overharvested, so this species faces a real danger of becoming vulnerable in its natural habitat. Ex situ conservation using in vitro tools is a possible solution to this problem. Ex situ conservation of plants involving in vitro tools has been initiated through axillary branching using nodal explants. Studies on in vitro storage under slow-growth conditions were carried out to develop an efficient protocol for conservation of A. racemosus germplasm. In vitro shoot cultures generally require a 4-wk subculture onto fresh medium when grown at 25±2°C under a 16-h photoperiod. In this research, the use of mannitol or sorbitol as an osmoticum and reduction of sucrose to 1.5% (w/v) in half-strength MS medium led to maintenance of the cultures for 6 mo at 25±2°C with no subculture. Surviving shoots from the slow-growth cultures could be regenerated with 100% efficiency, indicating that the subculture interval was successfully extended by this method. Temperature and medium modification both had significant effects on the growth of stored shoots, and the two factors showed significant interaction. In experiments designed to test encapsulation as a storage method, micropropagated shoot clusters encapsulated in calcium alginate beads were successfully stored up to 75 d at 25±2°C under a 16-h photoperiod. Stored shoots from both storage methods were subsequently recovered and multiplied on MS medium with 3% sucrose and 1.11 μM benzylaminopurine at 25 ± 2°C. Welldeveloped shoots were rooted and acclimatized successfully.

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Section: Resultsmentioning

confidence: 99%

In vitro approaches for conservation of Asparagus racemosus Willd.

Thakur

Tiwari

Jadhav

2015

In Vitro Cell.Dev.Biol.-Plant

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“…As the storage time increased the germination time of synthetic seeds was also increased. This indicates that these nodal segments slowly recovered from any damage that occurred during long-term storage (Sujatha and Kumari, 2008). Explant takes time to recover from cold storage stresses after return to proliferation culture conditions (Ballester et al, 1997;West et al, 2006).…”

Section: Effect Of Storage Duration On Synthetic Seed Germination Andmentioning

confidence: 93%

In vitro conservation and production of vigorous and desiccate tolerant synthetic seeds in Stevia rebaudiana

Ali¹

2012

J. Med. Plants Res.

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Artificial (Synthetic) seed production along with microprpagation can solve many problems with regeneration of Stevia rebaudiana, which produce tiny and non-viable seeds. Shoot tips and axillary buds nodal segments were used from in vitro cultures for encapsulation. The explants were taken from green houses and tissue culture laboratory of AgriBiotech Research Farms, Lahore, Pakistan. Different concentrations of sodium alginate and calcium chloride affect the shape and germination of artificial seeds. A gelling matrix of 3% sodium alginate and 100 mM calcium chloride was found most suitable for the formation of firm, clear and isodiametric ideal beads. No effect of the duration of sodium alginate treatment on bead formation was observed. However, timing of CaCl 2 treatment proved to be crucial with 100 mM CaCl 2, treatment time of 15 min was suitable for isodiametric bead formation. The synthetic seeds pre-treated with KN0 3 germinated earlier than seeds without pretreatment with KNO 3. Root formation took place after 15 days of shoot formation without addition of any auxin. In liquid medium, the frequency of conversion was high. Frequency to plantlet conversion of synthetic seeds decreased gradually as the storage duration at 4°C increased. After 15, 30, 45 and 60 days of storage, frequencies to plantlet conversion from encapsulated and non-encapsulated segments were 86,

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“…In addition, we reduce the problem of the geographical boundaries where it was possible to germinate such plants at any time of the year in the laboratory regardless of geographical location. Plant tissue culture also helps to preserve the desired genotypes from genetic changes or extinction (Sujatha and Kumari 2008).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

et al. 2016

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Plant tissue culture can readily be applied to the field of medicinal plants and secondary metabolites production. In this study, in vitro propagation protocol of Rumex cyprius Murb. was established. The highest shoot multiplication rate was observed from explants grown on MS medium supplemented with 1.5 mg/L BA (7.8 shoots per explant). For rooting, the maximum root induction rate was observed on MS medium supplemented with 1.5 mg/L IBA or 1.0 mg/L NAA. The longest roots were observed for plants grown on MS medium supplemented with 1.5 mg/L IBA. For callus induction, leaves from in vitro grown plants were cultured in various levels of 2,4-D or different combinations of BA and NAA. The highest fresh weight (5.86 g) of calli was observed at 1.5 and 0.5 mg/L BA and NAA, respectively. The second part of this work investigated the effects of abiotic factors (NaCl and Mannitol) and biotic factors (Yeast extract and Chitosan) on secondary metabolites accumulation of in vitro grown R. cyprius. Phenolic compounds were extracted, and the individual phenolic compounds and antioxidant activity of the extracts were determined of in vitro grown R. cyprius. The total phenol content and antioxidant activity increased significantly by the pervious factors. Reversed high-pressure liquid chromatography (RP-HPLC) was used to measure the effect of these factors on the contents of individual phenolic compound. New phenolic compounds (Gallic acid and Chlorogenic acid) not found in R. cyprius plants grown on control media were produced in R. cyprius plants grown on media supplemented with elicitors. Results indicate that using elicitors in plant tissue culture could be used to produce/enhance secondary metabolites accumulation in R. cyprius.

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Micropropagation, encapsulation and growth of Artemisia vulgaris node explants for germplasm preservation

Cited by 31 publications

References 33 publications

In vitro approaches for conservation of Asparagus racemosus Willd.

In vitro approaches for conservation of Asparagus racemosus Willd.

In vitro conservation and production of vigorous and desiccate tolerant synthetic seeds in Stevia rebaudiana

Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

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