Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.)

Abstract

Murashige and Skoog medium (MS) and modified Anderson's Rhododendron medium (mAN) were compared for in vitro shoot multiplication of three highbush blueberries 'Berkeley', 'Bluecrop' and 'Goldtraube'. All media contained 0.5 mg l −1 zeatin applied either alone or combined with 0.1, 1 and 5 mg l −1 IBA. In vitro rooting was induced using mAN medium supplemented with 0.8 mg l −1 IBA and 4 g l −1 activated charcoal. The results obtained showed that mAN medium is more suitable for in vitro multiplication of the selected highbush blueberry cultivars than MS medium. Low concentration of IBA (≤1 mg l −1) added in zeatinsupplemented mAN medium increases shoot multiplication efficiency of highbush blueberries in vitro and can be recommended for large-scale propagation of high-quality plants. MS medium induced partial or full necrosis of stems and leaves, which was more pronounced on media containing zeatin combined with increasing concentration of IBA. Rooting capacity of shoots varied widely among the tested blueberry cultivars. The highest rooting and acclimatization rates were achieved in 'Goldtraube' (82.8% and 91.8% respectively), and the lowest (10% and 66.7% respectively) were in 'Berkeley'.

Establishment of efficient adventitious shoots induction system and ex vitro rooting in Vaccinium corymbosum (Ericaceae)

“…The dependence of plant tissue on exogenous growth regulators to induce adventitious shoots was well documented in blueberry (Sharma & Thorpe 1989, Gajdošová et al 2006, Meiners et al 2007, Liu et al 2010. Many of them were reported that the combination of cytokinins and auxins to induce plant regeneration (Rowland & Ogden 1993).…”

“…These limitations can be overcome by the utilization of in vitro culture techniques, and the most effective propagation system for blueberries is micropropagation due to the rapid and continuous production of a large number of plants. Over the last three decades, in vitro culture propagation on the various basal medium using axillary bud proliferation and adventitious shoot regeneration with different explants has been achieved with varying success (Gajdošová et al 2006, Li et al 2006. The culture of blueberries in North America has been more than one hundred years up to now where is the origin place for blueberry.…”

Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices

“…Artificial/synthetic cytokinins zeatin, thidiazuron (TDZ), and 2-isopentenyladenine (2iP), have been used to promote in vitro shoot formation from internodal cuttings, with multiple reports that zeatin is the most effective at shoot regeneration [8,9]. Zeatin is still widely used in High Chilling (HC) cultures [8,10], where the media concentration range varies among publications between 0.5 and 4 mg/L [8,9,11,12]. The optimal amount is not only genotype-specific, it also depends on the desired application, as cytokinins affect meristem proliferation [13].…”

“…Light can also be considered a plant growth regulator as different light wavelengths, intensity and amounts can initiate intracellular signaling cascades for example causing conformational changes in the backbone of cellular receptors called phytochromes, which can then transition to their biologically active form [14,15]. and 4 mg/L [8,9,11,12]. The optimal amount is not only genotype-specific, it also depends on the desired application, as cytokinins affect meristem proliferation [13].…”

Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

“…However, Nezami-Alanagh et al (2017) fund that gluconate − had an adverse effect on in vitro pistachio plant growth. In high-bush blueberry (Vaccinium corymbosum L.), the use of MS medium induced STN, especially when 0.5 mg/l zeatin was used with higher concentrations (> 1 mg/l) of IBA, but when this was replaced by Anderson's rhododendron medium (Anderson 1984), STN was eliminated (Ružić et al 2012). Anderson's rhododendron medium, relative to MS medium, contains about one-quarter the concentration of K + , NH + 4 and NO − 3 (Suppl.…”