Micropropagation of Anacyclus pyrethrum and chemical profiling of the regenerated plants for pellitorine, the active principle

Abstract

The paper reports the development of a protocol for large-scale micropropagation of Anacyclus pyrethrum, an invaluable medicinal plant, and the presence of pellitorine, the active principle, in leaves and roots of regenerated plants. Three explants-cotyledonary nodes, hypocotyls and cotyledons-were compared for their shoot development/differentiation ability on Murashige and Skoog's (MS, Physiol Plant 15:473-497, 1962) medium supplemented with 1-10 lM of 6-benzylaminopurine (BAP) or kinetin (Kn). The best response (8.88 ± 0.28 shoots per explant) was elicited from the cotyledonary nodal explants on MS ? 2.5 lM Kn. The in vitro regenerated shoots continued to multiply on being sub-cultured on the same medium. Elongated shoots were cultured on MS medium augmented with different concentrations (0.2-15 lM) of 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) for induction of roots. The media augmented with 5 or 10 lM IAA or 5 lM NAA were the most effective for the initiation and proliferation of roots. The plantlets were successfully transferred to the following three potting mixtures: garden soil, vermiculite and garden soil (1:1), vermiculite, garden soil and perlite (1:1:1). Among these, the maximum number (75 %) of the transferred plants survived in the garden soil. The high performance liquid chromatographic analyses of roots and leaves of the regenerated plants as well as those from the native habitat revealed the presence of pellitorine in all, with the content being higher in the roots than the corresponding leaves.The developed protocol would be of use for production of A. pyrethrum plants throughout the year, and thus could become a perennial source of the herb and its active principle.

Terpenoid and flavonoid spectrum of in vitro cultures of Glycyrrhiza glabra revealed high chemical heterogeneity: platform to understand biosynthesis

“…The varied chemical spectrum is probably due to PGR effect as the root explants were of same age and origin and were grown under similar in vitro conditions. Recent works of on Anacyclus pyrethrum (Singh et al 2015) and Securinega suffruticose (Raj et al 2015) have demonstrated the effect of PGR combinations on plant differentiation and secondary metabolite accumulation.…”

In vitro propagation and chemical profiling of <i>Herminium lanceum</i> (Thunb. ex Sw.) Vuijk, a medicinally important orchid, for therapeutically important phenolic acids

“…The presence of secondary metabolites in higher concentrations in in vitro raised plants than in wild ones has also been reported earlier. For example, the concentrations of rosmarinic acid, chlorogenic acid, hydrobenzoic acid and pellitorine were higher in in vitro regenerated plants of Rosmarinus officinalis (Guo et al 2007), Saussurea involucrate (Yesil-Celiktas et al 2007), Habenaria edgeworthii (Giri et al 2011) and Anacyclus pyrethrum (Singh et al 2015), respectively, than in the Figure 2. HPLC profiles of the extracts of tubers and leaves of in vivo (A, B, respectively) and in vitro (C, D respectively), analyzed for the presence and contents of gallic acid (GA), p-hydrooxybenzoic acid (HBA), syringic acid (SA) and caffeic acid (CA).…”

Qualitative and quantitative analysis of Precocene II, estimation of enzymatic, nonenzymatic antioxidant, and cytotoxic potentials of methyl jasmonate-elicited shoot culture of Ageratum conyzoides Linn.

“…BA showed the best response, followed by KIN and subsequently Zeatin and TDZ. KIN was observed as the best for multiple shoot inductions in several Asteraceae members [32][33][34][35]. To produce increased amounts of secondary metabolites using in vitro culture, application of elicitors is a good choice.…”