Micropropagation of Cardiospermum Halicacabum

Babber, S.; K., Mittal; Ahlawat, R.; Varghese, T. M.

doi:10.1023/a:1013763208472

Cited by 14 publications

(6 citation statements)

References 5 publications

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“…Effect of BA concentration was clearly detected in the B. rex leaf cultures, since the amount of responsive explants and the number of shoots per cultured explant decreased as the BA concentration decreased. This effect was also described in Cardiospermum halicacabum cultures (Babber et al 2001) in which the regeneration rate was optimal with 4 mg dm -3 of BA. The opposite BA dose effect was found in the B.…”

Section: ⎯⎯⎯⎯supporting

confidence: 54%

Shoot Regeneration in Four Begonia Genotypes

Espino¹,

Linacero²,

Rueda³

et al. 2004

Biologia plant.

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In vitro regeneration of four Begonia genotypes, B. semperflorens, B. rex, B. × elatior, and hybrid of Begonia with unknown parents 'Tiger' was carried out starting from leaf and petiole segments as explants. Five Murashige and Skoog′s derived media were tested, three of them supplemented with α-naphthaleneacetic acid (NAA) and 6benzyladenine (BA), and the other two with NAA and kinetin (KIN) in different concentrations. Shoot regeneration was preferentially induced on the BA containing media, quantitative differences being observed among explants and genotypes.

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Section: ⎯⎯⎯⎯supporting

confidence: 54%

Shoot Regeneration in Four Begonia Genotypes

Espino¹,

Linacero²,

Rueda³

et al. 2004

Biologia plant.

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“…According to Jahan & Anis (2009), it is not economically feasible to propagate C. halicacabum from seed due to low germination (35–40%), low viability of seeds and much delayed rooting of seedlings. Due to the poor germinability of C. halicacabum seeds, steps have been taken to produce seedlings of this species through micropropagation techniques (Babber et al 2001). However, if germination percentage of C. halicacabum could be improved, it would be economically feasible to produce seedlings from seeds rather than using tissue culture methods to produce them.…”

Section: Introductionmentioning

confidence: 99%

Seed dormancy of Cardiospermum halicacabum (Sapindaceae) from three precipitation zones in Sri Lanka

Thusithana

Amarasekara²,

Jayasuriya

et al. 2020

Plant Biol J

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This study investigated seed germination of Cardiospermum halicacabum, a medicinally important invasive species. • We compared mass, moisture content (MC), dormancy and dormancy-breaking treatments and imbibition and germination of scarified and non-scarified seeds of C. halicacabum from a low-elevation dry zone (DZ), low-elevation wet zone (WZ1) and mid-elevation wet zone (WZ2) in Sri Lanka to test the hypothesis that the percentage of seeds with water-impermeable seed coats (physical dormancy, PY) decreases with increased precipitation. • Seed mass was higher in WZ2 than in DZ and WZ1, while seed MC did not vary among the zones. All scarified DZ, WZ1 and WZ2 and non-scarified DZ and WZ1 seeds imbibed water, but only a few non-scarified WZ2 seeds did so. When DZ and WZ1 seeds were desiccated, MC and percentage imbibition decreased, showing that these seeds have the ability to develop PY. GA 3 promoted germination of embryos excised from fresh DZ and WZ1 seeds and of scarified WZ2 seeds. • At maturity, seeds from DZ and WZ1 had only physiological dormancy (PD), while those from WZ2 had combinational dormancy (PY+PD). Thus, our hypothesis was not supported. Since a high percentage of excised embryos developed into normal seedlings; this is a low-cost method to produce C. halicacabum plants for medicinal and ornamental purposes.

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“…The in vitro culture protocol devised for micropropagation of C. halicacabum has been presented in literature with successful plant regeneration using either callus [3] , [4] or using meristematic explants such as nodal segments [5] . However, there was no report published based on direct plant regeneration from hypocotyls explants.…”

Section: Introductionmentioning

confidence: 99%

Relative examination of antioxidative enzymatic activities in plantlets of Cardiospermum halicacabum L. differentiated from hypocotyls in in vivo and ex vitro environment

Jahan

Anis

Aref

2014

Biotechnology Reports

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A plant regeneration protocol was devised for Cardiospermum halicacabum by means of aseptically extracted 7 days old hypocotyls forming adventitious shoots on Murashige and Skoog (MS) medium harmonized with 0.7 μM thidiazuron (TDZ) producing a maximum of 18.20 ± 0.98 number of shoots in 94% cultures following 4 weeks. Subsequent subculturing for five passages, on a medium without plant growth regulators, tempted the highest shoot number (40.00 ± 1.15) with an average shoot length of 6.53 ± 0.49 cm after the fourth subculture. Histological sections confirmed the formation of multiple buds from hypocotyl explants. The expression of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase was found to be higher in acclimatized plants than in the in vitro cultured ones suggesting the involvement of these enzymes in shoot differentiation and in growth under external environment partly due to their ability to cope up with oxidative stress.

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Micropropagation of Cardiospermum Halicacabum

Cited by 14 publications

References 5 publications

Shoot Regeneration in Four Begonia Genotypes

Shoot Regeneration in Four Begonia Genotypes

Seed dormancy of Cardiospermum halicacabum (Sapindaceae) from three precipitation zones in Sri Lanka

Relative examination of antioxidative enzymatic activities in plantlets of Cardiospermum halicacabum L. differentiated from hypocotyls in in vivo and ex vitro environment

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