Micropropagation of Juglans regia L

Leal, Darcy Ríos; Sánchez‐Olate, Manuel; Avilés, Fabiola; Materán, María Elena; Uribe, Matilde; Hasbún, Rodrigo; Rodríguez, R.

doi:10.1007/978-1-4020-6352-7_35

Cited by 8 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro regeneration methods using zygotic embryos enable overcoming the barriers in hybridization [11,12], obtaining higher and faster multiplication rates of plants of an elite genotype [10]. According to Lambardi et al [13] and Rios Leal et al [14], in vitro propagation is a method that creates opportunities for obtaining homogeneous plant material with high genetic stability if explants, such as apical buds or shoot tips, are used as initial plant material. The socioeconomic significance of walnut trees associated with their wide range of uses, requires the development of procedures to ensure the rapid regeneration of selected lines.…”

Section: Introductionmentioning

confidence: 99%

Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

Sota,

Benelli,

Myrselaj

et al. 2023

Horticulturae

View full text Add to dashboard Cite

Juglans regia L. is a crucial species as a forest tree and for its nutritional and medicinal values. It is also included in the list of endangered species in Albania and thus, there is a need to find methodologies to ensure its rapid regeneration and ex situ conservation. This research, investigated the regeneration of plantlets from synthetic seeds containing shoot tips of four native walnut varieties: ‘Përmet’, ‘Korçë’, ‘Peshkopi’, and ‘Tropojë’. First, in vitro-derived shoot tips from walnut seedlings are encapsulated using sodium alginate. After that, the regeneration potential of the encapsulated shoot tips and the influence of incubation conditions are evaluated. The synthetic seeds were incubated at either 25 °C or 8 °C, with and without dehydration treatment, in 0.5 M sucrose solution for 3 h. The synthetic seeds in both temperature regimes (25 °C and 8 °C) develop plantlets and provid conservation potential without the need for subcultures for 4 and 3.5 months, respectively. Furthermore, all walnut varieties incubated in these conditions achiev a high regeneration rates.

show abstract

Section: Introductionmentioning

confidence: 99%

Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

Sota,

Benelli,

Myrselaj

et al. 2023

Horticulturae

View full text Add to dashboard Cite

show abstract

“…They also found that adding penicillin and streptomycin to the nutrient medium prevented contamination (Fu et al, 2003). Leal et al (2007) stated that internal contamination is an important problem in micropropagation experiments with explants taken from mature walnut trees. In addition, they stated that darkening as a result of oxidation in the tissues during the surface sterilization stage caused a great reduction in shoot formation and this problem could be overcome by using young shoot explants that are more resistant to disinfection but are semi-woody (Leal et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Leal et al (2007) stated that internal contamination is an important problem in micropropagation experiments with explants taken from mature walnut trees. In addition, they stated that darkening as a result of oxidation in the tissues during the surface sterilization stage caused a great reduction in shoot formation and this problem could be overcome by using young shoot explants that are more resistant to disinfection but are semi-woody (Leal et al, 2007). Vahdati et al (2009) stated that the success of tissue culture depends on the chemical composition of the culture medium and the micropropagation of the walnut is partially limited by the lack of a suitable culture medium.…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes

DİRLİK>

Kandemir

ÇETİN>

et al. 2022

Gazi University Journal of Science Part A: Engineering and Innovation

View full text Add to dashboard Cite

The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the 'Paradox' (Juglans regia x J. hindsii) rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate (4 %). According to the results, SP4 medium provided the highest number of shoots per unit explant (1.05 shoots/explant) and 100 % shoot formation, SP2 medium provided the best mean shoot length (1.6 cm) and the mean number of nodes per unit explant (7.95 nodes/explant). Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod (3500 lux) for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite (1:1 v/v) and was rooting rate is 45.45 % in ¼DFe medium.

show abstract

“…The composite floor system referred to is part of a larger experimental programme that aims to evaluate the structural response of different types of thin-walled shear connector. Additional thin-walled shear connector solutions were designed and tested, including the perfobond-type thin-walled perfobond (TWP), which was submitted to preliminary push-out tests based on the Eurocode 4 [5] recommendations, as reported on by Leal and Batista [6], [7] and Leal [8].…”

Section: Introductionmentioning

confidence: 99%

Composite floor system with cold‐formed trussed beams and prefabricated concrete slab

Leal

Batista

2020

Steel Construction

View full text Add to dashboard Cite

Steel‐concrete composite floor systems represent a common solution for buildings, leading to reduced material consumption and improving the structural strength and flexural stiffness. Lightweight steel frame construction systems, based on the combination of thin‐walled cold‐formed steel members (CFS) and panels (e.g. wood, cementitious, gypsum plaster), may also benefit from the composite behaviour in the case of concrete slabs. In this context, full‐scale experimental tests were conducted to evaluate the structural behaviour of a floor system conceived with 0.95 mm thick CFS trussed beams and prefabricated concrete slabs. An innovative solution for shear connectors was designed and tested in order to ensure full interaction between the CFS trussed beam and the concrete slab. The thin‐walled channel (TWC) connector is based on a lipped channel CFS attached to the top chord of the truss by standard self‐drilling screws. Experimental results indicate efficient behaviour of the TWC shear connectors with improved bending capacity of the floor system.

show abstract

Micropropagation of Juglans regia L

Cited by 8 publications

References 15 publications

Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes

Composite floor system with cold‐formed trussed beams and prefabricated concrete slab

Contact Info

Product

Resources

About