Micropropagation of Lilium candidum L. : a rare and native bulbous flower of Turkey

Bürün, Betül; Şahin, Oral

doi:10.3329/bjb.v42i1.15913

Cited by 8 publications

(6 citation statements)

References 3 publications

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“…The bulblets we obtained on the media free of exogenous PGR showed a normal structure. We achieved a comparable or even higher organogenesis efficiency without PGRs than the other researchers (Khawar et al 2005;Burun and Sahin 2013;Akshay et al 2021). This indicates that it is possible to reduce the use of chemicals in in vitro bulb reproduction.…”

Section: Morphological Effects Of Different Spectral Combinations On ...supporting

confidence: 65%

“…This study on micropropagation of L. candidum showed intense formation of adventitious bulbs on the bulbscales growing on the media without exogenous growth regulators. Earlier papers on micropropagation of L. candidum investigated the effects of medium PGR on organogenesis (Khawar et al 2005;Burun and Sahin 2013;Saadon and Zaccai 2013;Akshay et al 2021). Some researchers noted the formation of abnormal bulblets and disturbed development of L. candidum leaves on the PGR supplemented media (Lian et al 2003;Saadon and Zaccai 2013).…”

Section: Morphological Effects Of Different Spectral Combinations On ...mentioning

confidence: 99%

“…Research has been conducted on the micropropagation of many species and cultivars of lily (Bakhshaie et al 2016), with just a few focusing on L. candidum. The studies mostly concern initiation of in vitro culture and elimination of numerous bacterial and fungal infections with antibiotics and fungicides toxic to the plant tissues (Altan et al 2010;Burun and Sahin 2013). Bulbscales are the best explants for efficient formation of adventitious organs in in vitro cultures of Lilium (Bakhshaie et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Pałka

Cioć

Hura

et al. 2022

Plant Cell Tiss Organ Cult

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Lilium candidum L. is a plant with rich cultural traditions and huge potential in floriculture and modern medicine. Our study analyzed the effects of light emitting diodes (LEDs) of variable quality on adventitious organogenesis induced in bulb scales placed on Murashige and Skoog medium. In addition to the spectral compositions of red (100%), blue (100%), and red and blue light (RB ratio 7:3), RB light was mixed in equal proportions (50%) with green (RBG), yellow (RBY), UV (RBUV), and far red (RBfR) light. We also tested white LED (Wled). Fluorescent lamp light (Fl) and darkness (D) served as controls. We assessed the effects of light on the morphometric features as well as the physiological and biochemical quality of the bulblets (adventitious bulbs). Bulb formation was observed under all light qualities, even in darkness, albeit to a limited extent. White LED light (Wled) treatment translated into the highest efficiency of bulblet formation and the greatest number of bulbs with developed leaves. The leaves, even though the shortest ones, also developed as a result of RBG treatment, and this light mixture enhanced the diameter of the forming bulblets. The bulbletsunder this light spectrum accumulated the most chlorophyll and carotenoids. The bulblets formed under B, RBfR and RBG LED had more than two times higher phenoliclevels than those formed under R LED and Fl. The bulbletsformed under B LED were the richest in soluble sugars, similarly to those developing in darkness (D).

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Section: Morphological Effects Of Different Spectral Combinations On ...supporting

confidence: 65%

Section: Morphological Effects Of Different Spectral Combinations On ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Pałka

Cioć

Hura

et al. 2022

Plant Cell Tiss Organ Cult

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“…The composition of plant growth regulators in the culture medium directs the organogenesis in plants (Parmaksiz and Khawar, 2006;Aasim et al, 2009;Daneshvar-Royandezagh et al, 2009). Similarly, (Burun et al, 2013) also noted bulblet regeneration from L. candidum bulb scales on MS containing different doses and combinations of NAA, BA, Kn and 2iP. However, they noted maximum bulblet induction of 88.2% in MS supplemented with 0.1 mg/l NAA + 0.01 mg/l BA and the average number of bulblets per explant was 2.9.…”

Section: Semi Solid Agar Containing Mediummentioning

confidence: 99%

Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate

Royandazagh

2019

Not Bot Horti Agrobo

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Lilium candidum L. is one of the most fragrant plant species among Liliaceae. The indigenous and natural populations of L. candidum are significantly affected by fast increasing anthropological population pressure and increased carbon fuel pollution. There is a necessity for development of a micropropagation protocol to serve in effective manner for both commercial multiplication and protection of the plant. The study targeted to develop a strategy for effective in vitro plant propagation system using static liquid culture, seismomorphogenic treatments with mixed liquid culture and semi solid culture medium using single scale explants. The former two techniques failed to regenerate new bulblets effectively whereby the later technique induced 100% regeneration on all treatments. Maximum number of bulblets regenerated on 0.27 µM Thidiazuron + 1.08 µM Naphthalene acetic acid. The scale explant could be recultured and regenerated on the mother culture medium for six cycles. The daughter bulblets regenerated on Murashige and Skoog medium containing both 0.18 or 0.27 µM TDZ + 1.08 µM NAA were successfully rooted using 1/2 × MS medium containing 2.45 µM indole butyric acid. Regardless of rooting development, acclimatization was noted on all bulblets in the greenhouse. These plants are showing continuous flowering since last four years under hot-summer Mediterranean (CSa type) climate field conditions of Tekirdag province of Thrace region in Turkey. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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“…Although there are many published papers for method development on somatic embryogenesis of Lilium spp., [13][14][15][16][17][18][19][20], none of them reported genetic stability investigation after in vitro propagated L. candidum natural populations using SSR molecular marker techniques. The current study was carried out to improve efficient micropropagation method for multiple shoot propagation via somatic embryogenesis induced from L. candidum stem bulbils and also to determine the genetic stability of in vitro grown plants using SSR markers.…”

Section: Introductionmentioning

confidence: 99%

Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR

Tokgöz

Altan

2020

International Journal of Secondary Metabolite

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Natural populations of Lilium candidum L. are remarkably affected by biotic and abiotic factors therefore there is a requirement to develop effective micropropagation protocol to provide mass production, multiplication and conservation of these plants. For this reason, this study was aimed to develop an efficient micropropagation method for multiple shoot production via somatic embryogenesis induced from L. candidum stem bulbils and also to determine the genetic stability of in vitro grown plants using SSR markers. The obtained results of this study are the first comprehensive reports including an investigation of genetic fidelity on somatic embryogenesis of L. candidum. After surface sterilization of bulbils, the calculated regeneration percentage of them was 89.5% and the callus induction was achieved using leaf segments of in vitro grown bulbils. The well formed somatic embryos were obtained from smooth whitish-yellow colored calli and these somatic embryos produced well formed healthy L. candidum seedlings with adventitious roots. All rooted seedlings were easily adapted to greenhouse conditions and the genetic stability of in vitro grown seedlings were determined by using SSR-PCR technique and it was calculated as 100%.

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Micropropagation of Lilium candidum L. : a rare and native bulbous flower of Turkey

Cited by 8 publications

References 3 publications

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate

Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR

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