MicroRNA‑106b functions as an oncogene and regulates tumor viability and metastasis by targeting LARP4B in prostate cancer

Abstract

Prostate cancer (PCa) is the most common malignancy among males worldwide, and is one of the leading causes of cancer-related mortality. MicroRNAs (miRs) are a type of endogenous, noncoding RNA that serve a key role in pathological processes, and have been demonstrated to be involved in the formation and progression of PCa. Previous studies have reported that miR-106b acts as an oncogene; however, the specific effects of miR-106b on PCa have not been fully elucidated. The present study aimed to investigate the role and underlying molecular mechanisms of miR-106b in the initiation and progression of PCa. In this study, miR-106b was reported to be overexpressed and la-related protein 4B (LARP4B) was downregulated in PCa tissues compared with paracancerous tissues. In addition, LARP4B was identified as a target gene of miR-106b by bioinformatics prediction analysis and a dual luciferase reporter gene assay. Furthermore, MTT, wound healing and Transwell assays were performed to evaluate PCa cell viability, and migration and invasive abilities. The data revealed that inhibition of miR-106b significantly suppressed the viability, migration and invasion of PCa cells. In addition, inhibition of miR-106b significantly suppressed the mRNA and protein expression of cancer-related genes, including matrix metalloproteinase-2, cluster of differentiation 44 and Ki-67, and increased that of the tumor suppressor, mothers against decapentaplegic homolog 2. Collectively, the findings of the present study indicated that miR-106b may target LAR4B to inhibit cancer cell viability, migration and invasion, and may be considered as a novel therapeutic target in PCa.

Circulating miRNAs as Potential Biomarkers in Prostate Cancer Patients Undergoing Radiotherapy

“… 47 MiR-106b has been shown to regulate apoptosis and focal adhesion-related pathways. 48 Also, miR-106b promotes cell proliferation and invasion 49 and in another study was shown that its hypoxia-induced over-expression results in a more aggressive phenotype. 46 It has also been shown that miR-106b is highly expressed in metastatic tissues compared to normal and is correlated with bone metastasis.…”

Analysis of Gene Expression of miRNA-106b-5p and TRAIL in the Apoptosis Pathway in Gastric Cancer

“…Fan et al [41] concluded in their work that miR-106b-5p expression was increased in cervical cancer, and by suppressing this microRNA, cancer could be inhibited through apoptosis of the malignant cells. Similarly, Yin et al [50] found an upregulation of miR-106b-5p in prostate cancer and concluded that inhibiting this microRNA could be an effective therapy against this cancer. We suggest that miR-106b-5p may act as an oncogene in gastric cancer, considering its high expression in the Cancer group and the ability to target genes in the apoptosis pathway and control them.…”

“…Studies have demonstrated that miR-106b-5p is related to cell proliferation, invasion, and metastasis in the esophagus, gastric, breast, hepatic, renal, and lung cancer [50][51][52]. Fan et al [41] concluded in their work that miR-106b-5p expression was increased in cervical cancer, and by suppressing this microRNA, cancer could be inhibited through apoptosis of the malignant cells.…”

Identification of Novel Genes Associated with Chronic Obstructive Pulmonary Disease Using Different Penalized Logistics Regression with High-Dimensional Biological Data from Human Sputum Cells

“…They also reported that STX6 may has a role in methylate process that is seen in this disease (28) In other studies, the association between PLCB1 and the decrease of FEV1 and FEV1/FVC in their participation was shown (29). Another study reported that PLCB1 at least in two pedigree with severe COPD was observed (30) Other group found an association between ZNF33A gen and remission from asthma(31) LARP4B is a target gene of miR106 inhibition of miR-106b that suppressed the mRNA and protein expression of cancer-related genes (32). Syntaxin 6 may have a role in regulating neutrophil secondary granule exocytosis also stimulation of cells by Ca2+.…”