MicroRNA-125b suppresses the migration and invasion of hepatocellular carcinoma cells by targeting transcriptional coactivator with PDZ-binding motif

Li, Jipeng; Fang, Laifu; Yu, Wanjun; Wang, Yiping

doi:10.3892/ol.2015.2973

Cited by 30 publications

(19 citation statements)

References 28 publications

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“…In terms of HCC, it has been reported that the levels of miR‐125b expression were downregulated in HCC tissues and cell lines, and that ectopic expression of miR‐125b reduced the cellular proliferation and cell cycle progression of HCC cells . In addition, miR‐125b suppresses the migration and invasion of hepatoma cells by directly targeting oncogene LIN28B and transcriptional coactivator with PDZ‐binding motif . Thus, this miRNA is indicated to exert anti‐oncogenetic effects in hepatic carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of plasma microRNA‐125b in hepatitis B virus‐related liver diseases and diagnostic potential for hepatitis B virus‐induced hepatocellular carcinoma

Chen

Gao

et al. 2016

Hepatology Research

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Section: Discussionmentioning

confidence: 99%

Differential expression of plasma microRNA‐125b in hepatitis B virus‐related liver diseases and diagnostic potential for hepatitis B virus‐induced hepatocellular carcinoma

Chen

Gao

et al. 2016

Hepatology Research

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“…miRNA 125b was shown to inhibit cell invasion and migration through targeting of eIF4EBP1 in ovarian cancer cells48 and to act as a transcriptional coactivator at the PSD-95, Discs-large, ZO-1-binding motif in hepatocellular carcinoma cells 49. miRNA-125b was reported to exert its tumor suppressor functions by targeting Sphk1 in bladder cancer,50 and to affect the PI3K/Akt/mTOR signaling pathway in cervical cancer 51.…”

Section: Discussionmentioning

confidence: 99%

A Pilot Study of Circulating MicroRNA-125b as a Diagnostic and Prognostic Biomarker for Epithelial Ovarian Cancer

Zhu¹,

Gao²,

Chen³

et al. 2016

Int J Gynecol Cancer

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ObjectiveEarly diagnosis of epithelial ovarian cancer is critical for patient survival. The objective of this pilot study is to identify a circulating micro (mi)RNA as a potential biomarker for epithelial ovarian cancer.MethodsA total of 135 epithelial ovarian cancer patients and 54 benign ovarian tumor patients were recruited for this study. Using customized TaqMan low density miRNA arrays, we first screened expression levels of 48 miRNAs in sera from 18 epithelial ovarian cancer patients and 16 benign ovarian tumor patients. The most significantly and differentially expressed miRNA was then further examined in all serum samples using real-time polymerase chain reaction. Its expression was further analyzed in relationship with clinicopathological factors and patient survival.ResultsArray screening data showed that expression levels of serum miRNA-20a, miRNA-125b, miRNA-126, miRNA-355, and let-7c were significantly different between malignant and benign ovarian tumor patients. Subsequent real-time polymerase chain reaction results showed that serum miRNA-125b levels were significantly higher in epithelial ovarian cancer patients compared to benign controls. Moreover, serum miRNA-125b levels were significantly higher in ovarian cancer patients in early stages I and II, and in patients having no residual tumor following surgery, but were not associated with differentiation and histological types of ovarian cancer. Notably, the higher level of miR-125b was significantly positively correlated with progression-free survival (P = 0.035) and marginally, with overall survival (P = 0.069).ConclusionsmiRNA-125b plays an important role in the pathogenesis and progression of epithelial ovarian cancer. Circulating miRNA-125b has the potential to become a novel biomarker for early diagnosis and prognosis prediction of epithelial ovarian cancer.

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“…Unlike in HepG2 cells, where Ets1 gene expression is high, therefore Ets1-inhibiting miRNA expression level is supposed to be decreased. Among the reported miRNA, miR-125b-5p was the only miRNA highly expressed in SMMC7721 cells but lowly expressed in HepG2 compared with other two groups of miRNA profiles (Li et al, 2015). Similar to miR-125b-5p, the other miRNAs with possible interaction with the 3 0 -UTR of Ets1 mRNA predicted by Target Scan program included miR-23b-3p, miR-26a-5p, and miR-423-5p.…”

Section: Differential Expression Of Mirnas Predicted To Bind To 3 0 -mentioning

confidence: 92%

“…Therefore, we used miRNA microassay to semiquantitatively analyze miRNA expression miR-23b-3p and miR-125b-5p downregulate apo(a) expression J-f. Zeng et al profile in the Hep G2 and SMMC7721 cells. Among the reported miRNA, miR-125b-5p was the only miRNA highly expressed in SMMC7721 cells but lowly expressed in HepG2 compared with other two groups of miRNA profiles (Li et al, 2015). High miRNA expression could induce the downregulation of related target gene.…”

Section: Differential Expression Of Mirnas Predicted To Bind To 3 0 -mentioning

confidence: 94%

miR‐23b‐3p and miR‐125b‐5p downregulate apo(a) expression by targeting Ets1 in HepG2 cells

Zeng

Zhang

et al. 2017

Cell Biology International

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High concentrations of plasma lipoprotein(a) [Lp(a)] have been inferred to be an independent risk factor for cardiovascular and cerebrovascular diseases, such as coronary artery diseases, restenosis, and stroke. Apolipoprotein(a) [apo(a)] is one of the most important components of Lp(a) and contributes greatly to the increased concentration of plasma Lp(a). As a critical positive transacting factor of apo(a) gene, Ets1 has been proven as a target gene of several miRNAs, such as miR-193b, miR-125b-5p, miR-200b, miR-1, and miR-499. In this study, a series of experiments on miRNAs and relative miRNAs inhibitor delivered HepG2 cells were conducted, and two miRNAs that downregulate the apo(a) by targeting the 3'-UTR of Ets1 were identified. Results showed that apo(a) and Ets1 were differentially expressed in SMMC7721 and HepG2 cell lines. Meanwhile, apo(a) and Ets1 were inversely correlated with several hepatic endogenous miRNAs, such as miR-125b-5p, miR-23b-3p, miR-26a-5p, and miR-423-5p, which were predicted to bind to Ets1. Results show that miR-125b-5p and miR-23b-3p mimics could inhibit the synthesis of apo(a) by directly targeting Ets1 in HepG2, thereby reducing the plasma Lp (a) concentration.

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MicroRNA-125b suppresses the migration and invasion of hepatocellular carcinoma cells by targeting transcriptional coactivator with PDZ-binding motif

Cited by 30 publications

References 28 publications

Differential expression of plasma microRNA‐125b in hepatitis B virus‐related liver diseases and diagnostic potential for hepatitis B virus‐induced hepatocellular carcinoma

Differential expression of plasma microRNA‐125b in hepatitis B virus‐related liver diseases and diagnostic potential for hepatitis B virus‐induced hepatocellular carcinoma

A Pilot Study of Circulating MicroRNA-125b as a Diagnostic and Prognostic Biomarker for Epithelial Ovarian Cancer

miR‐23b‐3p and miR‐125b‐5p downregulate apo(a) expression by targeting Ets1 in HepG2 cells

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