MicroRNA-132 Plays an Independent Prognostic Role in Pancreatic Ductal Adenocarcinoma and Acts as a Tumor Suppressor

Chen, Yan; Zhu, Huiyun; Wang, Yuqiong; Song, Ying-Xiao; Zhang, Pingping; Wang, Zhijie; Gao, Jun; Li, Zhao‐Shen

doi:10.1177/1533033818824314

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…This suggests that miR-132 expression is not induced by a pancreatogenic diabetes mellitus secondary to chronic pancreatitis. The connection of miR-132 expression and pancreatic cancer is controversially discussed in the literature, some studies reported a downregulation of the miR-132 expression in cancer tissue and cells [60][61][62] , while other studies confirmed an upregulation of miR-132 [63][64][65] . The hepatic expression of miR-132 is reported to be upregulated after alcoholic hepatitis 66 , nonalcoholic fatty liver disease (NAFLD), hepatic steatosis 67 , as well as cholestasis 68 .…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

Kumstel

Janssen-Peters

Abdelrahman

et al. 2020

Sci Rep

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Severity assessment of animal experiments is mainly conducted by using subjective parameters. A widely applicable biomarker to assess animal distress could contribute to an objective severity assessment in different animal models. Here, the distress of three murine animal models for gastrointestinal diseases was assessed by multiple behavioral and physiological parameters. To identify possible new biomarkers for distress 750 highly conserved microRNAs were measured in the blood plasma of mice before and after the induction of pancreatitis. Deregulated miRNA candidates were identified and further quantified in additional animal models for pancreatic cancer and cholestasis. MiR-375 and miR-203 were upregulated during pancreatitis and down regulated during cholestasis, whereas miR-132 was upregulated in all models. Correlation between miR-132 and plasma corticosterone concentrations resulted in the highest correlation coefficient, when compared to the analysis of miR-375, miR-203 and miR-30b. These results indicate that miR-132 might function as a general biomarker for distress, whereas the other miRNAs were altered in a disease specific manner. In conclusion, plasma miRNA profiling may help to better characterize the level of distress in mouse models for gastrointestinal diseases.

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Section: Discussionmentioning

confidence: 99%

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

Kumstel

Janssen-Peters

Abdelrahman

et al. 2020

Sci Rep

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“…Available evidence indicates that dysregulation of miRs can contribute to tumor progression and metastasis (12,13). Human miR-132, one of the miRs that can potentially regulate the expression of various tumor suppressor genes, including p53, is located on human chromosome 17 (14,15). The expression of miR-132 in LC, osteosarcoma and colorectal cancer tissue has been demonstrated to be lower compared with normal tissue (14)(15)(16).…”

Section: Microrna-132-3p Regulates Cell Proliferation Apoptosis Migmentioning

confidence: 99%

“…Human miR-132, one of the miRs that can potentially regulate the expression of various tumor suppressor genes, including p53, is located on human chromosome 17 (14,15). The expression of miR-132 in LC, osteosarcoma and colorectal cancer tissue has been demonstrated to be lower compared with normal tissue (14)(15)(16). In the present study, bioinformatics analysis predicted Sox4 as a potential target gene of miR-132 and it was hypothesized that over expression of miR-132 markedly inhibited cancer cell growth, invasion and migration as well as promoted cell apoptosis by targeting Sox4.…”

Section: Microrna-132-3p Regulates Cell Proliferation Apoptosis Migmentioning

confidence: 99%

MicroRNA‑132‑3p regulates cell proliferation, apoptosis, migration and invasion of liver cancer by targeting Sox4

Huang

Xiang

et al. 2020

Oncol Lett

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The present study investigated whether microRNA (miR)-132-3p targeted transcription factor SOX-4 (Sox4) for the inhibition of proliferation, migration, invasion and promotion of apoptosis in liver cancer (LC) cells. The expression of miR132-3p and Sox4 mRNA was evaluated by quantitative PCR and protein expression was determined by western blot analysis. Cell proliferation, apoptosis, migration, and invasion were assessed at different time points by the MTT assay, flow cytometry analysis, wound healing assay and Transwell migration assay, respectively. Bioinformatics prediction and luciferase assays were performed to validate and confirm Sox4as a potential target of miR-132p. There was a reduced expression of miR-132-3p in HepG2 and Huh7 cell lines compared with HccLM3 cells. Overexpression of miR-132-3p resulted in significant inhibition of proliferation and induction of apoptosis in LC cells. Moreover, migration and invasion of HepG2 cells were suppressed by over expressing miR-132-3p. However, downregulation of miR-132-3p in Hep-G2 cells promoted cell growth, invasion and migration and inhibited apoptosis. Bioinformatics analysis predicted Sox4 as a potential target of miR-132-3p, which was further confirmed by the luciferase reporter assay. In addition, an inverse association was observed between miR-132-3p and Sox4 expression. miR-132-3p may regulate the proliferation, apoptosis, migration and invasion of HepG2 cells by targeting Sox4.

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“…Mechanically, miRNAs usually cause mRNA degradation and/or translational inhibition by targeting mRNAs via directly binding to their 3′-untranslated regions (3′-UTRs) [ 9 ]. MiRNAs have been widely reported to be aberrantly expressed and play important roles in the development of cancer, including PDAC [ 10 – 12 ]. Recent studies showed that miR-33a (consisting of two single stranded miRNAs: miR-33a-5p and miR-33a-3p) is located in intron 16 of chromosome 22, which is involved in cholesterol biosynthesis and cholesterol uptake [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Tumor suppressive role of miR-33a-5p in pancreatic ductal adenocarcinoma cells by directly targeting RAP2A

2021

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Background The suppressive effects of miR-33a-5p have been reported in colorectal cancer and lung cancer. However, the functional role of miR-33a-5p in pancreatic ductal adenocarcinoma (PDAC) has not yet been elucidated. Methods The expression of miR-33a-5p was determined using reverse-transcription quantitative PCR (RT-qPCR) in PDAC tissues and cell lines. The association between miR-33a-5p expression and clinical categorical parameters was analyzed by the chi-square test. Cell proliferation was analyzing by Cell Counting Kit -8 (CCK-8) assay. Transwell assay was utilized to assess cell migration and invasion. The interactions between miR-33a-5p and RAP2A were verified by luciferase reporter assay, RT-qPCR, western blot analysis and RNA immunoprecipitation (RIP) assay. Results Here, we observed for the first time that miR-33a-5p expression level was significantly decreased in PDAC tissues and cell lines. There was a significant association between decreased miR-33a-5p expression and TNM stage or lymph node metastasis. Overexpression of miR-33a-5p significantly inhibited SW1990 and PANC-1 cell proliferation, migration and invasion. Knockdown of miR-33a-5p remarkedly promoted cell proliferation, migration and invasion in BxPC-3 and ASPC-1. Mechanistically, RAP2A was confirmed as the target of miR-33a-5p in PDAC cells. Moreover, RAP2A overexpression abolished miR-33a-5p-mediated suppressive effects on SW1990 and PANC-1 cells. Conclusions Taken together, these results suggest that miR-33a-5p exerted tumor suppressive effects on PDAC cells by targeting RAP2A, which might provide a new theoretical basis for the clinical treatment of PDAC.

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MicroRNA-132 Plays an Independent Prognostic Role in Pancreatic Ductal Adenocarcinoma and Acts as a Tumor Suppressor

Cited by 9 publications

References 18 publications

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases

MicroRNA‑132‑3p regulates cell proliferation, apoptosis, migration and invasion of liver cancer by targeting Sox4

Tumor suppressive role of miR-33a-5p in pancreatic ductal adenocarcinoma cells by directly targeting RAP2A

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