2016
DOI: 10.1007/s12035-015-9655-7
|View full text |Cite
|
Sign up to set email alerts
|

MicroRNA-146a Promotes Oligodendrogenesis in Stroke

Abstract: Stroke induces new myelinating oligodendrocytes that are involved in ischemic brain repair. Molecular mechanisms that regulate oligodendrogenesis have not been fully investigated. MicroRNAs (miRNAs) are small non-coding RNA molecules that post-transcriptionally regulate gene expression. MiR-146a has been reported to regulate immune response, but the role of miR-146a in oligodendrocyte progenitor cells (OPCs) remains unknown. Adult Wistar rats were subjected to the right middle cerebral artery occlusion (MCAo).… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
63
1

Year Published

2016
2016
2020
2020

Publication Types

Select...
8
2

Relationship

3
7

Authors

Journals

citations
Cited by 82 publications
(66 citation statements)
references
References 58 publications
2
63
1
Order By: Relevance
“…Fluorescence in situ hybridization (FISH) in combination with fluorescent immunostaining were performed according to a published protocol [30]. Briefly, the cells were fixed in 4% paraformaldehyde, and then hybridized with a miR-34a LNA (locked nucleic acid) probe, and the probe was detected with peroxidase-conjugated anti-FAM (Roche, Basel, Switzerland) followed by incubation tyramine-signal-amplification (TSA)-Cy3 substrate for 10 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…Fluorescence in situ hybridization (FISH) in combination with fluorescent immunostaining were performed according to a published protocol [30]. Briefly, the cells were fixed in 4% paraformaldehyde, and then hybridized with a miR-34a LNA (locked nucleic acid) probe, and the probe was detected with peroxidase-conjugated anti-FAM (Roche, Basel, Switzerland) followed by incubation tyramine-signal-amplification (TSA)-Cy3 substrate for 10 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…Furthermore, having in mind that microRNA (miRNA)āˆ’146a may directly induce OPC differentiation to OLs (Zhang et al, 2017a;Liu et al, 2016b), TĪ²4 treatment may prominently stimulate the expression of this mRNA in primary cultured OPCs, thus promoting the proliferation and differentiation of OPCs and OLs (Santra et al, 2014), which may serve as a common remyelination therapeutic mechanism. In addition to increasing miR-146a expression in OPCs, TĪ²4 remarkably also increased miR-146a expression in microglia and significantly inhibited secretion of pro-inflammatory mediators (Zhou et al, 2015), all of which may promote remyelination (Crockford, 2007).…”
Section: The Effects Of TĪ²4 On Opc Differentiation and Remyelination mentioning
confidence: 99%
“…Targeting other cells such as oligodendrocytes that may help better healing of ischemic region is also a useful treatment. MicroRNA 146a promotes oligodendrocytes in the ischemic region [51].…”
Section: Role Of Micrornas In Determining the Outcome Of Strokementioning
confidence: 99%