MicroRNA-16 Inhibits Bladder Cancer Proliferation by Targeting Cyclin D1

Abstract

MicroRNA-16 (miR-16) has been demonstrated to regulate proliferation and apoptosis in many types of cancers, but its biological function in bladder cancer remains unknown. Here, we found expression of miR-16 to be downregulated in bladder cancer in comparison with the adjacent normal tissues. Enforced expression of miR-16 was able to inhibit cell proliferation in TCHu-1 cells, in line with results for miR-16 antisense oligonucleotides (antisense miR-16). At the molecular level, our results further revealed that cyclin D1 expression was negatively regulated by miR-16. Therefore, the data reported here demonstrate that miR-16 is an important regulator in bladder cancer, which will contribute to better understanding of important mis-regulated miRNAs.

Role of miR-15/16 in CLL

“…29,31 Two other recent reports demonstrated that miR-15/16 directly target critical cell cycle regulator Cyclin D1 in bladder cancer and osteosarcoma. 32,33 Another study showed that miR-15/16 expression is downregulated in malignant pleural mesothelioma (MPM). 34 Administration of synthetic mimics to restore miR-15/16 expression led to growth inhibition in MPM cell lines and in xenograft-bearing nude mice; administration of miR-15/16 mimics led to consistent inhibition of MPM tumor growth.…”

Induction of MicroRNA-9 Mediates Cytotoxicity of Curcumin Against SKOV3 Ovarian Cancer Cells

“…It is well accepted that each miRNA can regulate the expression of many target mRNAs (Jiang et al, 2013;Ling et al, 2013). Several direct targets of miR-9 such as cyclin D1, Ets1 (Zheng et al, 2013), and CXCR4 (Lu et al, 2013) have currently been identified.…”

Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles

“…MTT assay showed that matrine suppressed the viability of human lung cancer line A549 in dose-and time-dependent manners after cells were treated with matrine at 0.25-4.0 mg/ml for 24, 48, 72 h, respectively. Cell cycle plays a critical role in regulating cell proliferation, growth as well as cell division (Wang et al, 2012;Hernandez-Hernandez et al, 2013;Jiang et al, 2013) . The cell cycle analysis showed that at duration matrine can induce cell cycle arrest at G0/G1 phase and simultaneously decreasing the G2/M phase.…”