MicroRNA-184 Modulates Human Central Nervous System Lymphoma Cells Growth and Invasion by Targeting iASPP

Liang, Xiaogong; Wei, Meng; Hu, Lianjie; Li, Lin; Xing, H. Rosie; Xie, Guiqing; Wang, An-qiong; Jia, Yongyi

doi:10.1002/jcb.25856

Cited by 15 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Micro-RNA-184 has been described as a tumor suppressor in various cancers (lymphoma [ 32 ], renal carcinoma [ 33 ], breast cancer [ 34 , 35 ], lung cancer [ 36 , 37 ], ovarian cancer [ 38 ], glioma [ 39 ], otorhinolaryngologic cancer [ 40 – 42 ], gastric cancer [ 43 ] or adrenocortical tumors [ 44 ]). Micro-RNA184 acts by repressing oncogenes such as TNFAIP2 [ 39 , 43 ], SND1 [ 45 ], CDC25A, c-MYC and Bcl-2 [ 36 ], and by regulating the AKT/mTORC1 pathway.…”

Section: Discussionmentioning

confidence: 99%

Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer

et al. 2018

View full text Add to dashboard Cite

BackgroundActual European pathological classification of early-stage endometrial cancer (EC) may show insufficient accuracy to precisely stratify recurrence risk, leading to potential over or under treatment. Micro-RNAs are post-transcriptional regulators involved in carcinogenic mechanisms, with some micro-RNA patterns of expression associated with EC characteristics and prognosis. We previously demonstrated that downregulation of micro-RNA-184 was associated with lymph node involvement in low-risk EC (LREC). The aim of this study was to evaluate whether micro-RNA signature in tumor tissues from LREC women can be correlated with the occurrence of recurrences.MethodsMicroRNA expression was assessed by chip analysis and qRT-PCR in 7 formalin-fixed paraffin-embedded (FFPE) LREC primary tumors from women whose follow up showed recurrences (R+) and in 14 FFPE LREC primary tumors from women whose follow up did not show any recurrence (R−), matched for grade and age. Various statistical analyses, including enrichment analysis and a minimum p-value approach, were performed.ResultsThe expression levels of micro-RNAs-184, -497-5p, and -196b-3p were significantly lower in R+ compared to R− women. Women with a micro-RNA-184 fold change < 0.083 were more likely to show recurrence (n = 6; 66%) compared to those with a micro-RNA-184 fold change > 0.083 (n = 1; 8%), p = 0.016. Women with a micro-RNA-196 fold change < 0.56 were more likely to show recurrence (n = 5; 100%) compared to those with a micro-RNA-196 fold change > 0.56 (n = 2; 13%), p = 0.001.ConclusionsThese findings confirm the great interest of micro-RNA-184 as a prognostic tool to improve the management of LREC women.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer

et al. 2018

View full text Add to dashboard Cite

show abstract

“…MiR-184 has been extensively explored in various cancers. Decreased miR-184 expression levels were noted in gliomal cells [14,15], central nervous system lymphoma cells [16] and renal cell carcinoma [17]. By contrast, miR-184 expression was found to be upregulated in pancreatic ductal adenocarcinoma [18], hepatocellular carcinoma [19] and tongue squamous cell carcinoma [20].…”

Section: Introductionmentioning

confidence: 99%

miR-184 Inhibits Tumor Invasion, Migration and Metastasis in Nasopharyngeal Carcinoma by Targeting Notch2

Zhu¹,

Jiang

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: A recent study found that dysregulated microRNA-184 (miR-184) is involved in the proliferation and survival of nasopharyngeal carcinoma (NPC). This study aimed to evaluate the detailed mechanisms of invasion, migration and metastasis of NPC cells. Methods: Quantitative reverse-transcription PCR (qRT-PCR) and Western blot were used to confirm the expression levels of miR-184 and Notch2. NPC cell invasion and migration were subsequently examined using in vitro cell invasion and wound-healing assays, respectively. MicroRNA (miRNA) target gene prediction databases and dual-luciferase reporter assay were adopted to validate the target genes of miR-184. Results: MiR-184 was downregulated in the NPC cell lines. The miR-184 inhibitor increased the number of invading NPC cells, whereas miR-184 mimics inhibited the invasive ability of such cells. The protein level of E-cadherin decreased, whereas those of N-cadherin and vimentin increased in the anti-miR-184 group. This result showed that miR-184 inhibited NPC cell invasion and metastasis by regulating EMT progression. MiRNA target gene prediction databases indicated the potential of Notch2 as a direct target gene of miR-184. Such a notion was then validated by results of dual-luciferase reporter assay. Notably, shRNANotch2 restrained the EMT and partially abrogated the inhibitory effects of miR-184 on EMT progression in NPC cells. Conclusion: MiR-184 functions as a tumour-suppressive miRNA targeting Notch2 and inhibits the invasion, migration and metastasis of NPC.

show abstract

“…MiR-184 is abnormally expressed in a variety of tumor tissues. In addition to osteosarcoma, miR-184 is also involved in the proliferation and invasion of various malignant tumor types, including glioblastoma, renal cell carcinoma and central nervous system lymphoma ( 19 ). However, the existing mechanism of action requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Regulatory effects of microRNA‑184 on osteosarcoma via the Wnt/β‑catenin signaling pathway

Wang

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

The present study aimed to investigate the role of microRNA (miRNA/miR)-184 in osteosarcoma growth, development and metastasis, and the effects of miRNA-184 on the proliferation, invasion and metastasis of osteosarcoma cells and associated mechanisms. In vitro, miR-184 was transfected into U-2OS cells and 143B cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-184. MTT was utilized to detect cell proliferation. A Transwell assay was applied to detect cell invasiveness. In vivo, an osteosarcoma tibial orthotopic metastatic tumor model was established, and western blotting and RT-qPCR were used to detect the expression of Wnt and β-catenin. Following the overexpression of miR-184, the proliferation and cell invasion ability were significantly increased in U-2OS and 143B cells. Following inhibition of miR-184, cell proliferation and cell invasion ability were significantly decreased. In nude mice, tumor volume significantly increased following overexpression of miR-184, and Wnt and phosphorylated β-catenin levels were significantly increased. Following miR-184 inhibition, tumor volume was significantly decreased, and Wnt and phosphorylated β-catenin levels were significantly decreased. The results of the present study indicated that the Wnt/β-catenin signaling pathway serves a key function in the mechanism of osteosarcoma. Inhibition of miRNA-184 may reduce tumor volume of osteosarcoma via regulation of the Wnt/β-catenin signaling pathway and may provide a novel strategy for the future diagnosis and treatment of osteosarcoma.

show abstract

MicroRNA-184 Modulates Human Central Nervous System Lymphoma Cells Growth and Invasion by Targeting iASPP

Cited by 15 publications

References 35 publications

Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer

Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer

miR-184 Inhibits Tumor Invasion, Migration and Metastasis in Nasopharyngeal Carcinoma by Targeting Notch2

Regulatory effects of microRNA‑184 on osteosarcoma via the Wnt/β‑catenin signaling pathway

Contact Info

Product

Resources

About