Regulatory effects of microRNA‑184 on osteosarcoma via the Wnt/β‑catenin signaling pathway

Abstract

The present study aimed to investigate the role of microRNA (miRNA/miR)-184 in osteosarcoma growth, development and metastasis, and the effects of miRNA-184 on the proliferation, invasion and metastasis of osteosarcoma cells and associated mechanisms. In vitro, miR-184 was transfected into U-2OS cells and 143B cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-184. MTT was utilized to detect cell proliferation. A Transwell assay was applied to detect cell invasiveness. In vivo, an osteosarcoma tibial orthotopic metastatic tumor model was established, and western blotting and RT-qPCR were used to detect the expression of Wnt and β-catenin. Following the overexpression of miR-184, the proliferation and cell invasion ability were significantly increased in U-2OS and 143B cells. Following inhibition of miR-184, cell proliferation and cell invasion ability were significantly decreased. In nude mice, tumor volume significantly increased following overexpression of miR-184, and Wnt and phosphorylated β-catenin levels were significantly increased. Following miR-184 inhibition, tumor volume was significantly decreased, and Wnt and phosphorylated β-catenin levels were significantly decreased. The results of the present study indicated that the Wnt/β-catenin signaling pathway serves a key function in the mechanism of osteosarcoma. Inhibition of miRNA-184 may reduce tumor volume of osteosarcoma via regulation of the Wnt/β-catenin signaling pathway and may provide a novel strategy for the future diagnosis and treatment of osteosarcoma.

Regulation of microRNAs by rape bee pollen on benign prostate hyperplasia in rats

“…The rno‐miR‐184 has dual functions in cancers. It is an onco‐miRNA in osteosarcoma (Du, Li, Wang, Huang, & Xu, ), squamous cell carcinoma of tongue (Wong et al, ), but acts as a tumour suppressor in colorectal cancer (Wu, Liu, Wu, Wu, & Yao, ), breast cancer (Fu et al, ), even acts as both onco‐miRNA (Wu et al, ) and suppressor miRNA in glioma (Cheng et al, ). Although rno‐miR‐184 is an onco‐miRNA in prostate cancer (Lin, Chiang, Chang, & Ying, ; Walter, Valera, Pinto, & Merino, ), there is no evidence about relationship between rno‐miR‐184 and BPH.…”

Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line

“…Of the group of 18 upregulated miRNAs (Let-7c, Let-7d, Let-7e, Let-7f, miR-135b, miR-221, miR-184, miR-18a-5p, miR-200, miR-1, miR-9, miR-744, miR-296-5p, miR-199a-3p, miR-320, miR-654, miR-141-3p and miR-369-3p), only miR-1, Let-7d, miR-320, miR-221, miR-184, miR-141-3p, miR-18a-5p, miR-135b, Let-7a, miR-199a-3p, miR-365, and miR-744 had been investigated [ 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 ] in OS before now.…”

“…miR-184 has been described to be closely related to tumor occurrence and progression in several tumors [ 97 , 98 ], among which OS. Du Z. et al, investigating the role of miR-184 in OS cell lines, have demonstrated that miR-184 is responsible for an abnormal cellular proliferation by upregulating Wnt and β-catenin mRNA expression levels [ 69 ]. Yin G. et al reported that miR-184 is highly expressed in OS cell lines and demonstrated that the inhibition of this leads to a suppression of cell proliferation [ 72 ], confirming the oncogene behavior of this miRNA, as has been described in other malignant tumors [ 99 ].…”

The impact of miR-9 in osteosarcoma

“…Many studies found that Wnt signaling pathway plays an important role in the development of osteosarcoma. Du et al, [ 44 ] found that inhibition of miRNA- 184 may reduce tumor volume of osteosarcoma by regulating the Wnt /β- catenin signaling pathway. Zhang et al, [ 45 ] suggested that miR-107 inhibit development of osteosarcoma via the Wnt signaling pathway, and the critical role of miR- 214 in human osteosarcoma also through involving the Wnt signaling pathway.…”