MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1

Xie, Fang; Hosany, Sumayyah; Zhong, Shen; Jiang, Yang; Zhang, Fen; Lin, Lili; Wang, Xiaobo; Gao, Shenmeng; Hu, Xiaoqu

doi:10.1371/journal.pone.0185565

Cited by 69 publications

(55 citation statements)

References 34 publications

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“…24,25 miR-193a-5p was usually considered as an anti-cancer factor. Its anti-cancer effects in hepatocellular carcinoma, 26 glioblastoma, 27 breast cancer 28 and other cancers have been confirmed. In our study, miR-193a-5p has been shown to inhibit the malignant phenotypes of CRC cells, thereby inhibiting the progression of CRC.…”

Section: Discussionmentioning

confidence: 87%

<p>Long Non-Coding RNA Cancer Susceptibility 9 (CASC9) Up-Regulates the Expression of ERBB2 by Inhibiting miR-193a-5p in Colorectal Cancer</p>

Ding

Zhang

et al. 2020

CMAR

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Background: Emerging studies have reported that long non-coding RNAs (lncRNAs) were crucial regulators in the progression of colorectal cancer (CRC). LncRNA susceptibility 9 (CASC9) was involved in several cancers; however, its role in CRC remains unknown. Methods: RT-PCR was done to probe the expression of CASC9 and miR-193a-5p in CRC samples. CRC cell lines (HCT116 and SW480) were used as cell models. The biological influence of CASC9 on cancer cells was studied using CCK-8 assay, Transwell assay and TUNEL assay in vitro, and subcutaneous xenotransplanted tumor model in vivo. Interaction between CASC9 and miR-193a-5p was investigated by bioinformatics analysis, RT-PCR, and luciferase reporter assay. The expression level of the downstream gene of miR-193a-5p, erb-b2 receptor tyrosine kinase 2 (ERBB2), was tested by Western blot. Results: CASC9 was significantly up-regulated in CRC samples, while miR-193a-5p was markedly down-regulated. Overexpression of CASC9 promoted viability, migration and invasion of CRC cells, while overexpression of miR-193a-5p had the opposite effect. CASC9 could down-regulate miR-193a-5p via sponging it, and there was a negative relevancy between CASC9 and miR-193a-5p in CRC samples. CASC9 also enhanced the expression levels of ERBB2, while this effect could be reversed by co-transfection with miR-193a-5p. Conclusion: CASC9, an oncogenic lncRNA, was abnormally up-regulated in CRC tissues, and it could indirectly modulate the expression of ERBB2 via reducing the expression level of miR-193a-5p.

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Section: Discussionmentioning

confidence: 87%

<p>Long Non-Coding RNA Cancer Susceptibility 9 (CASC9) Up-Regulates the Expression of ERBB2 by Inhibiting miR-193a-5p in Colorectal Cancer</p>

Ding

Zhang

et al. 2020

CMAR

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“…Previous studies have shown that six miRNAs, miR-2392, miR-362-5p, miR-193a-5p, miR-143-3p, miR-493-5p, and miR-600, were closely associated with invasion and cancer metastasis. [14][15][16][17][18][19] Then we used qRT-PCR to validate the expression levels of six miRNAs in SACC cells after transfection. Our results showed that the expression levels of miR-193a-5p and miR-143-3p were significantly upregulated in SACC-LM cells when ADAMTS9-AS2 was deleted, yet miR-193a-5p and miR-143-3p were downregulated in SACC-83 cells when ADAMTS9-AS2 was overexpressed, indicating a potentially competing relationship between the ADAMTS9-AS2 and miR-193a-5p or miR-143-3p ( Figures S2A and S2B).…”

Section: Adamts9-as2 Binds Mir-143-3p and Depresses Its Expressionmentioning

confidence: 99%

Upregulation of lncRNA ADAMTS9-AS2 Promotes Salivary Adenoid Cystic Carcinoma Metastasis via PI3K/Akt and MEK/Erk Signaling

Xie

et al. 2018

Molecular Therapy

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Neurotropic infiltrative growth and distant metastasis are the main causes of death in salivary adenoid cystic carcinoma (SACC) patients. Long noncoding RNAs (lncRNAs) are involved in many human neoplasms, however, their potential roles in SACC are unclear. In our study, we found that ADAM metallopeptidase with thrombospondin type 1 motif, 9 (ADAMTS9) antisense RNA 2 (ADAMTS9-AS2) was significantly upregulated in SACC patients with metastasis and SACC-lung metastasis (LM) cells. Moreover, ADAMTS9-AS2 expression was closely associated with the prognosis and distant metastasis in SACC patients. Next, we found that c-myc could specifically bind to the promoter of ADAMTS9-AS2 and activated its transcription. Knockdown of ADAMTS9-AS2 significantly inhibited migration and invasion of SACC cells in vitro and distant lung metastasis in vivo. Furthermore, ADAMTS9-AS2, which mainly expressed in the cytoplasm, shared microRNA (miRNA) response elements with Integrin α6 (ITGA6). Overexpression of ADAMTS9-AS2 competitively bound to miR-143-3p that inhibited ITGA6 from miRNA-mediated degradation, and thus it activated the activity of PI3K/Akt and MEK/Erk signaling and facilitated SACC metastasis. In summary, ADAMTS9-AS2 promotes migration and invasion in SACC by competing with miR-143-3p. This sheds a new insight into the regulation mechanism of ADAMTS9-AS2, and it provides a possible application for the SACC treatment.

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“…The metastasis of breast cancer cells requires several essential steps, including epithelial-mesenchymal transition (EMT) (3) and extracellular matrix degradation (4). Studies over the past decade have demonstrated that aberrant microRNA (miRNA) expression is closely associated with a variety of biological processes in breast cancer, including cell proliferation (5,6), apoptosis (7,8), metastasis (9,10) and chemosensitivity (11,12). MicroRNAs (miRNAs) are single-stranded RNA molecules typically 21-25 nucleotides in length, that can negatively regulate target gene expression.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39

Wang

Chen

et al. 2020

Oncol Rep

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Numerous studies have demonstrated that microRNAs (miRNAs) play a key role in human carcinogenesis and metastasis. For example, miR-299-5p has previously been revealed to be dysregulated in several human cancers. However, the biological function of miR-299-5p in breast cancer remains unclear. The present study demonstrated that miR-299-5p was downregulated in breast cancer tissues and cell lines. The restoration of miR-299-5p expression suppressed cell migration and invasion, whereas inhibition of miR-299-5p promoted cell migration and invasion. In addition, in vivo studies demonstrated that miR-299-5p overexpression was able to inhibit tumour metastasis in nude mice. Mechanistically, through bioinformatics analysis and a dual-luciferase assay, it was confirmed that miR-299-5p directly targets serine/threonine kinase 39 (STK39). Silencing STK39 inhibited cell metastasis and suppressed epithelial-mesenchymal transition markers and matrix metalloproteinase expression, whereas restoration of STK39 expression was able to reverse miR-299-5p-inhibited cell migration and invasion. Collectively, the results of the present study demonstrated that miR-299-5p supresses breast cancer cell migration and invasion by targeting STK39. These findings may provide novel insights into miR-299-5p and its potential diagnostic and therapeutic benefits in breast cancer.

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MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1

Cited by 69 publications

References 34 publications

<p>Long Non-Coding RNA Cancer Susceptibility 9 (CASC9) Up-Regulates the Expression of ERBB2 by Inhibiting miR-193a-5p in Colorectal Cancer</p>

<p>Long Non-Coding RNA Cancer Susceptibility 9 (CASC9) Up-Regulates the Expression of ERBB2 by Inhibiting miR-193a-5p in Colorectal Cancer</p>

Upregulation of lncRNA ADAMTS9-AS2 Promotes Salivary Adenoid Cystic Carcinoma Metastasis via PI3K/Akt and MEK/Erk Signaling

MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39

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