MicroRNA-196b promotes gastric cancer progression by targeting ECRG4

Abstract

Gastric cancer is one of the most common malignant tumors. MicroRNA-196b (miR-196b) has been demonstrated to play important roles in human cancers. However, its functions in gastric cancer progression were still largely unknown. In this study, the expression of miR-196b was determined by quantitative real-time PCR. Esophageal cancer-related gene 4 (ECRG4) level was examined by western blot assay and immunohistochemistry staining assay. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell migration and invasion were analyzed by transwell assay. The association between miR-196b and ECRG4 was analyzed by dual-luciferase reporter assay. The functional role of miR-196b in vivo was analyzed by murine xenograft assay. As a result, we found the expression of miR-196b was elevated and the protein expression of ECRG4 was reduced in gastric cancer tissues and cells. MiR-196b inhibition suppressed gastric cancer cell proliferation, migration and invasion. ECRG4 was a target of miR-196b and its protein expression was negatively regulated by miR-196b. Moreover, ECRG4 overexpression showed similar effects with miR-196b inhibition on the malignant behaviors of GC cells and ECRG4 knockdown reversed the effects of miR-196b inhibition on gastric cancer cell proliferation, migration and invasion. In addition, miR-196b inhibition suppressed tumor volume and weight in vivo. In conclusion, downregulation of miR-196b inhibited gastric cancer progression by modulating ECRG4 expression, indicating that miR-196b might be a potential therapeutic target for gastric cancer.

Identification hub genes of consensus molecular subtype correlation with immune infiltration and predict prognosis in gastric cancer

“…However, our results exhibited that high expression of ECRG4 was remarkably associated with poor outcomes (HR = 1.95, log-rank P = 6.7e−10), and its expression increased gradually in GC advance. The opposite result may be on account of different levels of evidence or cohort studies [ 36 , 38 ]. The deep reasons are yet to be further validated.…”

Identification Hub Genes of Consensus Molecular Subtype Correlation With Immune Infiltration and Predict Prognosis in Gastric Cancer

“…However, our results exhibited that high ECRG4 expression was remarkably associated with poorer outcomes (HR=1.95, log-rank P=6.7e-10), and its expression showing a state of gradual increase in GC advance. The opposite result may be on account of different levels of evidence or cohort studies [34,36]. The deep reasons yet to be further validated.…”

Identification of serum microRNAs as potential diagnostic biomarkers for detecting precancerous lesions of gastric cancer

“…This study identified the serum miRNA, miR‐196b‐3p , in subjects with precancerous lesions of GC by microarray analysis. The miR‐196 gene family ( miR‐196a‐1 , miR‐196a‐2 , miR‐196b ) is transcribed from a region harboring three genes, ECRG4 , 23 RAD23B , 24 and HOXA10 in the human homeobox (HOX) gene cluster region. miR‐196a‐1 and miR‐196a‐2 have the same mature nucleotide sequence, but mature miR‐196b differs from mature miR‐196A by only one nucleotide.…”

“…The Kimura-Takemoto classification was used for visual evaluation. Among the 144 subjects, there were 19 with C-0, 23 2B). Grade 3 in this classification is an advanced stage of chronic gastritis, which is defined as a precancerous lesion of GC.…”