MicroRNA-215 impairs adipocyte differentiation and co-represses FNDC3B and CTNNBIP1

Peng, Yanjie; Li, Huanan; Li, Xianglong; Yu, Shulong; Xiang, Hong; Peng, Jian; Jiang, Siwen

doi:10.1016/j.biocel.2016.08.014

Cited by 28 publications

(22 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FNDC3B belongs to the FN3 family (Syed et al, 2019). Research had presented that FNDC3B can enhance adipogenesis (Peng et al, 2016) and restrain osteoblast differentiation (Cao, Mitchell, Gorski, Hollinger, & Hoppman, 2016). A study reflected FNDC3B was upregulated in breast carcinoma tissues and was associated with carcinoma cell migration (Kong et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation

Chen

Shao

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Various circular RNAs (circRNAs) have been reported to involve in carcinoma. This study explored the role and mechanism of circRNA circFNDC3B (circFNDC3B) in renal carcinoma (RC). The detection indicators in this paper were viability, colony, and migration, which respectively investigated by Cell Counting Kit-8, colony formation, and migration assay. Reverse transcriptase quantitative polymerase chain reaction tested and cell transfection changed circFNDC3B and miR-99a expression. Moreover, western blot tested relate-proteins of proliferation, migration, and cell pathways were examined by western blot. circFNDC3B was upregulated at RC tissues. circFNDC3B enhanced cell viability, colony and migration, and miR-99a mimic played reverse impacts. Furthermore, circFNDC3B negatively regulated miR-99aand circFNDC3B restrained the janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and extracellular signal-regulated kinase (ERK) kinase (MEK)/ERK pathways by miR-99a downregulation.Overexpression of circFNDC3B enhanced cell viability, colony formation and migration by miR-99a downregulation via JAK1/STAT3 and MEK/ERK pathways. K E Y W O R D ScircFNDC3B, migration, miR-99a, renal carcinoma, viability

show abstract

Section: Discussionmentioning

confidence: 99%

Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation

Chen

Shao

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…For example, the miR‐23a~miR27a~24‐2 cluster represses bovine adipocyte differentiation (Wang et al, 2018), bta‐miR‐130a‐b affects bovine adipocyte differentiation by targeting PPARγ and CYP2UI (Ma et al, 2018), miR‐144‐3p suppresses preadipocyte proliferation (Shen, Li et al, 2018), and miR‐143, the miR‐17‐92 cluster, miR‐24, and miR‐30c induce adipocyte differentiation by negatively regulating extracellular signal‐regulated kinase 5 (Esau et al, 2004). Similarly, miR‐26b, miR‐27b, miR‐200b, and miR‐215 repress adipocyte differentiation by regulating PPARγ (Karbiener et al, 2009), KLF4 (Shen, Gan et al, 2018), PTEN (Song et al, 2014), FNDC3B , and CTNNBIP1 (Peng et al, 2016), respectively.…”

Section: Introductionmentioning

confidence: 99%

Bta‐miR‐149‐5p inhibits proliferation and differentiation of bovine adipocytes through targeting CRTCs at both transcriptional and posttranscriptional levels

Khan

Raza

Junjvlieke

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

MicroRNAs are small, single stranded, and noncoding RNAs that have been proven to be potent regulators of adipogenesis. However, the role of bta‐miR‐149‐5p in regulating bovine adipogenesis is still unclear. Expression profiling in different stages of adipogenesis revealed that bta‐miR‐149‐5p was enriched in the proliferation stage, and also on Day 9 of differentiation in bovine adipocytes. Our gain of function study showed that bta‐miR‐149‐5p can negatively regulate both bovine adipocyte proliferation and differentiation. Overexpression of bta‐miR‐149‐5p suppressed the expression of proliferation marker genes at both the messenger RNA (mRNA) and protein levels, markedly decreased the percentage of S‐phase cells, decreased the number of EdU‐stained cells, and substantially reduced adipocyte proliferation vitality in the cell count assay. Collectively, these findings elucidated that bta‐miR‐149‐5p inhibits adipocyte proliferation. Furthermore, overexpression of bta‐miR‐149‐5p also suppressed the expression of adipogenic genes at both the mRNA and protein levels, inhibited lipid accumulation, and reduced the secretion of adiponectin in bovine adipocytes. Furthermore, a luciferase activity assay explored how bta‐miR‐149‐5p targeted CRTCs (CRTC1 and CRTC2) directly. This targeting was further validated by the mRNA and protein level expression of CRTC1 and CRTC2, which were down regulated by bta‐miR‐149‐5p overexpression. Moreover, bta‐miR‐149‐5p indirectly targeted CRTC1 and CRTC2 through regulating their key transcription factors. Overexpression of bta‐miR‐149‐5p suppressed the expression of SMAD3, while enriched the expression of NRF1, which are the key transcription factors and proven regulators of CRTC1. Overexpression of bta‐miR‐149‐5p also repressed the expression of C/EBPγ, XBP1, INSM1, and ZNF263, which are the key regulators of CRTCs, at both the mRNA and protein levels. These findings suggest that bta‐miR‐149‐5p is a negative regulator of CRTC1 and CRTC2 both at transcriptional and posttranscriptional level. Taken together, these findings suggest that bta‐miR‐149‐5p can regulate adipogenesis, which implies that bta‐miR‐149‐5p could be a target for increasing intramuscular fat in beef cattle.

show abstract

“…For instance, miR-17-92 cluster, miR-204, miR-30e, miR-223 and miR-148a-3p promote adipocyte differentiation by negatively regulating retinoblastoma family (pRb) gene Rb2/p130, runt-related transcription factor 2 (Runx2), low-density lipoprotein receptor-related protein 6 (Lrp6), fibroblast growth factor receptor 2 (Fgfr2) and lysinespecific demethylase 6b (Kdm6b) respectively (Wang et al 2008, Huang et al 2010, Guan et al 2015, Tian et al 2017. miRNA let-7, miR-448, miR-24, miR-302a and miR-215 impair adipocyte differentiation by suppressing high-mobility group AT-hook 2 (Hmga2), Klf5, fatty acid-binding protein 4 (Fabp4, also termed aP2), PPARγ, fibronectin type III domain-containing 3B (Fndc3b) and catenin, beta-interacting protein 1 (Ctnnbip1) respectively (Sun et al 2009, Kinoshita et al 2010, Kang et al 2013, Jeong et al 2014, Peng et al 2016.…”

Section: Introductionmentioning

confidence: 99%

miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3

Zhu¹,

Zhang²,

Zhou³

et al. 2018

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

miR-20a-5p has recently been identified to induce adipogenesis of established adipogenic cell lines in our previous study. However, its role and molecular mechanisms in the regulation of adipocyte lineage commitment of bone marrow-derived stromal cells (BMSCs) still need to be explored. In this report, we demonstrated the expression of miR-20a-5p was promoted gradually during adipogenic differentiation in BMSCs. We also confirmed that miR-20a-5p has a positive function in the adipogenic differentiation of BMSCs by gain-of-function study with overexpression lentivirus or synthetic mimics of miR-20a-5p, and loss-of-function study with sponge lentivirus or synthetic inhibitor of miR-20a-5p. Dual luciferase reporter assay, GFP repression assay and Western blotting suggested Kruppel-like factor 3 () was a direct target of miR-20a-5p. Furthermore, siRNA-mediated silencing of recapitulated the potentiation of adipogenesis induced by miR-20a-5p overexpression, whereas enhanced expression of attenuated the effect of miR-20a-5p. As was reported to play an inhibitory role in adipogenesis at the initial stage of differentiation, the findings we present here indicate that miR-20a-5p promotes adipocyte differentiation from BMSCs by targeting and negatively regulating in the early phase during the procedure of adipogenesis.

show abstract

MicroRNA-215 impairs adipocyte differentiation and co-represses FNDC3B and CTNNBIP1

Cited by 28 publications

References 59 publications

Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation

Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation

Bta‐miR‐149‐5p inhibits proliferation and differentiation of bovine adipocytes through targeting CRTCs at both transcriptional and posttranscriptional levels

miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3

Contact Info

Product

Resources

About