MicroRNA-25 functions in regulation of pigmentation by targeting the transcription factor MITF in alpaca (Lama pacos) skin melanocytes

Zhu, Zhiwei; He, Junlin; Jia, Xiaoyun; Jiang, Junbing; Bai, Rui; Yu, Xiuju; Lv, Lei; Fan, Rong; He, Xu; Geng, Jie; You, Ran; Dong, Yanjun; Qiao, Dan; Lee, K.-B.; Smith, George W.; Dong, Chunhong

doi:10.1016/j.domaniend.2009.10.004

Cited by 86 publications

(71 citation statements)

References 29 publications

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“…Thus, a natural-colored wool is much preferred in the modern industry. Coat color is determined mainly by the synthesis and distribution of melanin, which is the main contributor to pigmentation in skin, hair bulbs, and the color of eyes (De Luca et al 1988;Zhu et al 2010). At the cellular level, melanin is synthesized in melanocytes, unique cells located in the basal layer of the epidermis and regularly interspersed among the basal keratinocytes.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, microphthalmia-associated transcription factor (MITF) is a master regulator of melanocyte development and melanogenesis in melanocytes (Fitzpatrick and Breathnach 1963;Fitzpatrick 1965;Lin et al 2007;Schallreuter et al 2008). MITF regulates the transcription of three major pigmentation enzymes, tyrosinase (TYR), tyrosine related protein-1 (TYRP1), and tyrosine related protein-2 (TYRP2; dopachrometautomerase, DCT) (Bentley et al 1994;Hemesath et al 1994;Yasumoto et al 1994;Zhu et al 2010). Overexpression of MITF increases the production of TYR, TYRP1, and DCT (Yasumoto et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse model

Dong¹,

Wang²,

et al. 2012

RNA

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Coat color is a key economic trait in wool-producing species. Color development and pigmentation are controlled by complex mechanisms in animals. Here, we report the first production of an altered coat color by overexpression of miR-137 in transgenic mice. Transgenic mice overexpressing miR-137 developed a range of coat color changes from dark black to light color. Molecular analyses of the transgenic mice showed decreased expression of the major target gene termed MITF and its downstream genes, including TYR, TYRP1, and TYRP2. We also showed that melanogenesis altered by miR-137 is distinct from that affected by UV radiation in transgenic mice. Our study provides the first mouse model for the study of coat color controlled by miRNAs in animals and may have important applications in wool production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse model

Dong¹,

Wang²,

et al. 2012

RNA

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“…Although the inverse association of miR-25 with MITF has been found in skin melanocytes of alpacas, 4 our study is the first to reveal an inverse correlation between miR-218 and MITF in melanocytes of human skin tissues, suggesting that the same interaction exists in vivo. In addition to its melanogenic activity, MITF is a lineage-addiction oncogene that plays a key role in melanocyte homeostasis and the pathogenesis of melanoma.…”

Section: Discussionmentioning

confidence: 73%

“…For example, miR-434-5p mediated skin whitening by targeting TYR in human and mouse melanocytes, 3 and miR-25 suppressed MITF in alpaca (Lama pacos) skin melanocytes. 4 A recent study also reported that miR-145 regulates depigmentary activity through the suppression of MYO5A expression in mouse and human melanocytes. 5 As a critical transcriptional factor, MITF knockout affects many miRNAs expression profiles in melanocytes.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-218 inhibits melanogenesis by directly suppressing microphthalmia-associated transcription factor expression

et al. 2014

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“…However, very little information is available regarding the expression of genes and limited research has been conducted to characterize global gene expression in alpaca skin. An example of the limited research on gene expression in alpaca skin includes a previous study from our group showing differential expression of micropthalmiaassociated transcription factor gene in skin tissues from alpacas of different coat colors (Zhu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Gene expression profile in white alpaca (Vicugna pacos) skin

Fan

Dong

Cao

et al. 2011

Animal

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A cDNA library from white alpaca (Vicugna pacos) skin was constructed using SMART technology to investigate the global gene expression profile in alpaca skin and identify genes associated with physiology of alpaca skin and pigmentation. A total of 5359 high-quality EST (expressed sequence tag) sequences were generated by sequencing random cDNA clones from the library. Clustering analysis of sequences revealed a total of 3504 unique sequences including 739 contigs (assembled from 2594 ESTs) and 2765 singletons. BLAST analysis against GenBank nr database resulted in 1287 significant hits ( E-value , 10 210 ), of which 863 were annotated through gene ontology analysis. Transcripts for genes related to fleece quality, growth and coat color (e.g. collagen types I and III, troponin C2 and secreted protein acidic and rich in cysteine) were abundantly present in the library. Other genes, such as keratin family genes known to be involved in melanosome protein production, were also identified in the library. Members (KRT10, 14 and 15) of this gene family are evolutionarily conserved as revealed by a cross-species comparative analysis. This collection of ESTs provides a valuable resource for future research to understand the network of gene expression linked to physiology of alpaca skin and development of pigmentation.

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MicroRNA-25 functions in regulation of pigmentation by targeting the transcription factor MITF in alpaca (Lama pacos) skin melanocytes

Cited by 86 publications

References 29 publications

Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse model

Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse model

MicroRNA-218 inhibits melanogenesis by directly suppressing microphthalmia-associated transcription factor expression

Gene expression profile in white alpaca (Vicugna pacos) skin

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