MicroRNA‑342 inhibits cell proliferation and invasion in nasopharyngeal carcinoma by directly targeting ZEB1

Zhu, Xiaoning; Li, Wei; Zhang, Renxian; Liu, Yutao

doi:10.3892/ol.2018.8788

Cited by 10 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed that cell viability and invasion were promoted by miR-342-3p silence and inhibited by miR-342-3p overexpression in HTR8/SVneo cells. Different with studies in T2DM, 34 miR-342-3p silence have an inhibition effect on PE progression. Trophoblasts normally migrate from the placenta into uterine spiral arteries, and invasiveness and metastasis of trophoblast cells will be altered by miRNA in PE.…”

Section: Discussioncontrasting

confidence: 87%

MiR‐342‐3p inhibition promotes cell proliferation and invasion by directly targeting ID4 in pre‐eclampsia

Han

Niu

Zuo

et al. 2019

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim This study aimed to explore the miR‐342‐3p expression in pre‐eclampsia (PE) placentas and confirm whether miR‐342‐3p exerts effects on proliferation and migration of HTR‐8/SVneo trophoblastic cells. Methods The PE placentas (n = 8) were taken from gravidas complicated by PE and delivered after 34 weeks. The chorionic plates and the basal plates were separately taken from the placenta disc near the position of umbilical cord insertion. RT‐qPCR was used to measure the expression of miR‐342‐3p in the chorionic plates and the basal plates. Cell invasion assay and MMT assay were used to assess the effects of miR‐342‐3p on proliferation and migration of HTR‐8/SVneo trophoblastic cells. Luciferase reporter assay and Western blotting were used to analyze the target of miR‐342‐3p and investigate the detailed mechanisms. Results The expression of miR‐342‐3p was upregulated in both basal plates and chorionic plates in patients with PE compared with healthy pregnant individuals. MiR‐342‐3p inhibitor suppressed the cell viability and invasion, and induced apoptosis in trophoblast cells. Furthermore, inhibitor of DNA binding (ID)‐4 (ID4) was a direct target of miR‐342‐3p, and knockdown of ID4 abrogated the regulation effect of miR‐342‐3p on cell viability, apoptosis and invasion. Conclusion Inhibition of miR‐342‐3p expression may suppress the occurrence of PE by targeting ID4 in vitro.

show abstract

Section: Discussioncontrasting

confidence: 87%

MiR‐342‐3p inhibition promotes cell proliferation and invasion by directly targeting ID4 in pre‐eclampsia

Han

Niu

Zuo

et al. 2019

J of Obstet and Gynaecol

View full text Add to dashboard Cite

show abstract

“…For example, miR-184 inhibits NPC cells to migrate and invade by modulating Notch2 (7). miR-342 directly inhibits the growth and metastasis of NPC cells through targeting ZEB1 (8). miR-495 downregulates GRP78 expression by regulating epithelial-mesenchymal transition (EMT), thus enhancing the radiotherapy-sensitivity of NPC (9).…”

Section: Introductionmentioning

confidence: 99%

miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A

Sun

Zang

et al. 2019

Oncol Lett

View full text Add to dashboard Cite

Biological function of microRNA-20c-3p (miRNA-520c-3p) in the progression of nasopharyngeal carcinoma (NPC) and the potential mechanism were investigated. Relative level of miRNA-520c-3p in NPC tissues and adjacent normal tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Particularly, miRNA-520c-3p level in NPC with different tumor stages and tumor sizes was examined. Subsequently, miRNA-520c-3p level in nasopharyngeal epithelial cells and NPC cells was detected. The potential influence of miRNA-520c-3p on the proliferative ability and cell cycle progression of NPC cells were evaluated through cell counting kit-8 (CCK-8) and flow cytometry. The target gene of miRNA-520c-3p was verified by dual-luciferase reporter gene assay. Regulatory role of miRNA-520c-3p/RAB22A in the malignant progression of NPC was identified. miRNA-520c-3p was downregulated in NPC tissues and cell lines. Its level was lower in NPC with worse tumor grade and larger tumor size. Overexpression of miRNA-520c-3p suppressed the proliferative ability and arrested cell cycle in G0/G1 phase. RAB22A was confirmed to be the downstream target of miRNA-520c-3p. In NPC tissues and cell lines, RAB22A remained in higher abundance relative to controls. Overexpression of RAB22A reversed the inhibitory effects of overexpressed miRNA-520c-3p on proliferative ability and cell cycle progression of NPC cells. miRNA-520c-3p is downregulated in NPC, which accelerates the malignant progression of NPC by targeting RAB22A.

show abstract

“…In addition, miRNAs can also act as an oncogenes or tumor-suppressor genes, and their role in the development of gliomas acts on the mechanisms of glioma (17). Downregulation of miR-342 has been found in a number of cancer types, such as breast cancer (18), prostate cancer (19) and nasopharyngeal carcinoma (20). Thus, it has been proposed that miR-342 could be used as a diagnostic and prognostic biomarker.…”

Section: Discussionmentioning

confidence: 99%

miR‑342 inhibits glioma cell proliferation by targeting GPRC5A

et al. 2019

View full text Add to dashboard Cite

accumulating evidence suggests that micrornas (mirnas) play a key role in the biological behaviors and progression of glioma. However, the function and bio-molecular mechanisms of mir-342 in glioma remain largely unclear. in the present study, reverse transcription quantitative-polymerase chain reaction and western blotting were performed to determine the mrna and protein expression levels of the factors investigated. MTT assay was performed to examine the proliferation rates. luciferase reporter assay was performed to test the binding between mirna-342 and its putative target. data indicated that mir-342 expression was markedly decreased in human glioma tissues and cell line u87, and reduced mir-342 expression significantly promoted cell proliferation. In order to explore the mechanisms, G-protein coupled receptor family C group 5 member A (GPRC5A) was identified as a target of mir-342 and depletion of GPrc5a suppressed cell proliferation. Our findings demonstrated that miR-342 regulates the cell proliferation of glioma by targeting GPrc5a, which indicates that mir-342 is a target of interest regarding the treatment of refractory glioma, and it may provide a promising prognostic and therapeutic strategy for glioma treatment.

show abstract

MicroRNA‑342 inhibits cell proliferation and invasion in nasopharyngeal carcinoma by directly targeting ZEB1

Cited by 10 publications

References 40 publications

MiR‐342‐3p inhibition promotes cell proliferation and invasion by directly targeting ID4 in pre‐eclampsia

MiR‐342‐3p inhibition promotes cell proliferation and invasion by directly targeting ID4 in pre‐eclampsia

miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A

miR‑342 inhibits glioma cell proliferation by targeting GPRC5A

Contact Info

Product

Resources

About