MicroRNA-375 overexpression disrupts cardiac development of Zebrafish (Danio rerio) by targeting notch2

Zhuang, Sisi; Fu, Yanrong; Li, Jingyun; Li, Mengmeng; Hu, Xiaoshan; Zhu, Jingai; Tong, Meiling

doi:10.1007/s00709-020-01490-4

Cited by 10 publications

(5 citation statements)

References 43 publications

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“…The consequences of this multivariate landscape of gene expression variability on the final phenotype, especially with regards to the effects of hybridization, remain unexplored. On the one hand, changes in the expression variability of genes from independent pathways may generate hybrid trait mismatches analogous to what is commonly described for average trait values, Epithelia of pharyngeal arches, head skeleton and pectoral fins, epidermis of head, tip of the tail, brain, spinal cord thymic primordium, eyes, sense organs, in zebrafish/medaka 67,101 Cell metabolism and viability in zebrafish 102 Lens pigments epithelial cell proliferation in in newt 103 Cardiac development in zebrafish 104 Vascular development, zebrafish 105 Involved in chondrogenesis, human Brain, sense organs, eyes, spinal cord, skeletal muscles, gills, excretory/digestive system, pharyngeal arches, fins, epidermis of the head, tip of the tail, in zebrafish/medaka 67,101 Brain morphogenesis in zebrafish 107 Angiogenesis in muscles, in zebrafish 108 Maternal at 200τ s miR-1* (25) Body, head, fin muscles and skeletal muscles, in zebrafish/ medaka 67,101 PL-dominant at 150τ s miR-100 (43), miR-181 (47) Brain; spinal cord, eyes, thymic primordium, sense organs, gills in zebrafish/medaka 67,101 a Different miRNAs from the same family, the same miRNAs with different orientations, paralogs or putative orthologs (the reads have aligned to different sequences, from different species, but have the same miRNAs name). b Putative location/functions according to the literature.…”

Section: Discussionmentioning

confidence: 89%

Rapid and biased evolution of canalization during adaptive divergence revealed by dominance in gene expression variability during Arctic charr early development

Horta-Lacueva,

Jónsson,

Thorholludottir

et al. 2023

Commun Biol

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Adaptive evolution may be influenced by canalization, the buffering of developmental processes from environmental and genetic perturbations, but how this occurs is poorly understood. Here, we explore how gene expression variability evolves in diverging and hybridizing populations, by focusing on the Arctic charr (Salvelinus alpinus) of Thingvallavatn, a classic case of divergence between feeding habitats. We report distinct profiles of gene expression variance for both coding RNAs and microRNAs between the offspring of two contrasting morphs (benthic/limnetic) and their hybrids reared in common conditions and sampled at two key points of cranial development. Gene expression variance in the hybrids is substantially affected by maternal effects, and many genes show biased expression variance toward the limnetic morph. This suggests that canalization, as inferred by gene expression variance, can rapidly diverge in sympatry through multiple gene pathways, which are associated with dominance patterns possibly biasing evolutionary trajectories and mitigating the effects of hybridization on adaptive evolution.

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Section: Discussionmentioning

confidence: 89%

Rapid and biased evolution of canalization during adaptive divergence revealed by dominance in gene expression variability during Arctic charr early development

Horta-Lacueva,

Jónsson,

Thorholludottir

et al. 2023

Commun Biol

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“…Further, miR-375 is pivotal for insulin-secreting island cells development in zebrafish (Kloosterman et al, 2007). In addition, miR-375 exerts an important role in proliferation, apoptosis, and cardiac morphogenesis of zebrafish embryos (Wang et al, 2016;Zhuang et al, 2020).…”

Section: Discussionmentioning

confidence: 98%

Insights Into miRNA-mRNA Regulatory Mechanisms of Cold Adaptation in Gymnocypris eckloni: Ubiquitin-Mediated Proteolysis Is Pivotal for Adaptive Energy Metabolism

Nie

Wang

et al. 2022

Front. Genet.

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This study aimed to understand cold stress adaptations mechanism in fish. Thus, the transcriptional response to cold conditions in Gymnocypris eckloni was evaluated using RNA-seq and microRNA (miRNA)-seq analyses. Low-temperature (LT) group G. eckloni was cultivated outdoors in waters cooled to 2–4°C for 3 weeks, while individuals in the control temperature (CT) group were exposed to 14–16°C. Significantly different responses were observed in both mRNA and miRNA expression profiles, with more mRNAs (1,833 and 1,869 mRNAs were up- and downregulated, respectively) and fewer miRNAs (15 and 6 were up- and downregulated, respectively) observed in the LT group individuals relative to the CT group individuals. A miRNA-mRNA network involved in the regulation of G. eckloni responses to cold stress was constructed; this network included ubiquitin-mediated proteolysis, protein processing, and oxidative phosphorylation. These results provided new insights into mechanisms of cold tolerance by fish, including decreased metabolic activity in addition to proteolysis.

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“…The absence of the miRNA processing enzyme Dicer can lead to abnormal formation of the heart outflow tract and chambers in mammals, so miRNA is essential for heart development ( Saxena and Tabin, 2010 ). Previous studies have revealed that a variety of miRNAs are involved in processes such as heart development and cardiomyocyte proliferation and differentiation, which are closely related to CHDs, including VSD ( Wang et al, 2016 ; Toni et al, 2020 ; Zhuang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA: Myocardium-derived prenatal biomarker of ventricular septal defects

Yang

Lian

et al. 2022

Front. Genet.

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Background: Recently, circulating microRNAs (miRNAs) from maternal blood and amniotic fluid have been used as biomarkers for ventricular septal defect (VSD) diagnosis. However, whether circulating miRNAs are associated with fetal myocardium remains unknown.Methods: Dimethadione (DMO) induced a VSD rat model. The miRNA expression profiles of the myocardium, amniotic fluid and maternal serum were analyzed. Differentially expressed microRNAs (DE-microRNAs) were verified by qRT–PCR. The target gene of miR-1-3p was confirmed by dual luciferase reporter assays. Expression of amniotic fluid-derived DE-microRNAs was verified in clinical samples.Results: MiRNAs were differentially expressed in VSD fetal rats and might be involved in cardiomyocyte differentiation and apoptosis. MiR-1-3p, miR-1b and miR-293-5p were downregulated in the myocardium and upregulated in amniotic fluid/maternal serum. The expression of amniotic fluid-derived DE-microRNAs (miR-1-3p, miR-206 and miR-184) was verified in clinical samples. Dual luciferase reporter assays confirmed that miR-1-3p directly targeted SLC8A1/NCX1.Conclusion: MiR-1-3p, miR-1b and miR-293-5p are downregulated in VSD myocardium and upregulated in circulation and may be released into circulation by cardiomyocytes. MiR-1-3p targets SLC8A1/NCX1 and participates in myocardial apoptosis. MiR-1-3p upregulation in circulation is a direct and powerful indicator of fetal VSD and is expected to serve as a prenatal VSD diagnostic marker.

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MicroRNA-375 overexpression disrupts cardiac development of Zebrafish (Danio rerio) by targeting notch2

Cited by 10 publications

References 43 publications

Rapid and biased evolution of canalization during adaptive divergence revealed by dominance in gene expression variability during Arctic charr early development

Rapid and biased evolution of canalization during adaptive divergence revealed by dominance in gene expression variability during Arctic charr early development

Insights Into miRNA-mRNA Regulatory Mechanisms of Cold Adaptation in Gymnocypris eckloni: Ubiquitin-Mediated Proteolysis Is Pivotal for Adaptive Energy Metabolism

Circulating microRNA: Myocardium-derived prenatal biomarker of ventricular septal defects

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