MicroRNA-384 represses the growth and invasion of non-small-cell lung cancer by targeting astrocyte elevated gene-1/Wnt signaling

Fan, Ni; Zhang, Jin; Cheng, Chuantao; Zhang, Xinwu; Feng, Jie; Kong, Ranran

doi:10.1016/j.biopha.2017.08.143

Cited by 27 publications

(24 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acquired evidence indicate that miRNAs play essential roles in cancer development, including osteosarcoma [20]. Although the roles of miR-384 in many types of cancers are investigated previously [15,16], the effect of miR-384 on osteosarcoma is rarely reported. The present study focused on the function role and the underlying mechanism of miR-384 in osteosarcoma.…”

Section: Discussionmentioning

confidence: 99%

“…Aberrant expression of miR-384 has been found in some kinds of cancers, including renal cell carcinoma [15], nonsmall-cell lung cancer [16], colorectal cancer [17] and hepatocellular carcinoma [18], indicating that miR-384 might play a critical role in tumorigenesis. Moreover, it exerted anti-proliferation, anti-invasion, anti-metastasis, and pro-apoptotic activities in these cancers via regulating its target genes like AEG1, KRAS, CDC42, and IRS1 [15][16][17][18], revealing the cancer suppressive role of miR-384.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP

Wang

Huang

2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Osteosarcoma is a common malignant bone tumour in adolescents and old people, with highly invasive and metastatic features and poor prognosis. This study aimed to explore the role of miR-384 in osteosarcoma MG63 cells by targeting SLBP. Cell viability, migration and invasion, apoptosis, as well as apoptosis-related factors were evaluated by CCK-8 assay, Transwell assay, flow cytometer and Western blotting, respectively. Dual-luciferase reporter assay was used to determine the target of miR-384. SLBP level was analyzed using qRT-PCR and Western blotting. Important factors of MEK/ERK and PI3K/AKT signalling pathways were analyzed using Western blotting. We found that miR-384 was down-regulated in osteosarcoma tissue samples and cell lines (MG63, U2OS and OS732). miR-384 overexpression inhibited viability, migration and invasion, but promoted apoptosis of MG63 cells; whereas, miR-384 silence exhibited the contrary effects on MG63 cells. SLBP was a target of miR-384. Knockdown of SLBP reversed the promoting effect of miR-384 silence on cells, indicating that miR-384 silence promoted growth and metastasis of MG63 cells by up-regulating SLBP. In conclusion, knocking down miR-384 promoted the growth and metastasis of osteosarcoma MG63 cells by up-regulating SLBP. To conclude, miR-384-SLBP may be a potential therapeutic target for osteosarcoma therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP

Wang

Huang

2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

show abstract

“…MiRNAs are short non-coding RNAs that can negatively regulate target gene expression by binding to the 3ʹ untranslated region (UTR) of the mRNAs through complementary base pairing, and inducing decay or transcriptional repression. 9 MiRNAs have multiple targets that regulate proliferation, differentiation, apoptosis, protein secretion and viral infection, 10,11 and are therefore attractive therapeutic targets. Several miRNAs involved in the development and progression of liver disease have also been identified that can be potential markers for liver cancer diagnosis, prognosis and pharmacogenomics.…”

Section: Introductionmentioning

confidence: 99%

<p>Mechanism of the Regulatory Effect of Overexpression of circMTO1 on Proliferation and Apoptosis of Hepatoma Cells via miR-9-5p/NOX4 Axis</p>

Wang¹,

Tan²,

Wang³

et al. 2020

CMAR

View full text Add to dashboard Cite

To investigate the potential role of the circMTO1/miR-9-5p/NOX4 axis in liver cancer. Materials and Methods: Human genome-wide circrna microarray V2 was used for analyzing the expression profile of circRNAs in human tissue samples. The TargetScan database was used to predict target genes. Gene overexpression and silencing in hepatoma cell lines were achieved by transfecting the cells with suitable constructs. Quantitative real time PCR and Western blotting were used to analyze gene and protein expression levels. CCK-8 analysis was performed to detect cell proliferation and the transwell assay for analyzing cell migration. Annexin V-FITC/PI staining and immunohistochemistry were respectively used to detect apoptosis and protein expression. Results: CircMTO1 were down-regulated in the liver cancer tissues and cell lines compared to their respective normal controls. TargetScan database screening and dual luciferase assay revealed that circMTO1 was a molecular sponge of miR-9-5p, and NOX4 was the target gene of miR-9-5p. Overexpression of circMTO1 and NOX4 inhibited proliferation and migration of hepatoma cells, while the overexpression of miR-9-5p had the opposite effects. In contrast, overexpression of circMTO1 and NOX4 promoted apoptosis, while that of miR-9-5p decreased the cell apoptosis rates. Conclusion: Overexpression of CircMTO1 acts as tumor suppressor in liver cancer by sponging miR-9-5p, which upregulates NOX4.

show abstract

“…Through this mechanism, miRNAs modulate >50% of all human protein-coding genes and implicated in the regulation of numerous physiological and pathological processes, including cell proliferation, cell cycle, apoptosis, differentiation and metastasis (7)(8)(9). However, abnormal expression of miRNAs has been observed in human malignancies, such as glioma (10), gastric cancer (11), lung cancer (12), cervical cancer (13) and bladder cancer (14). In tumourigenesis and tumour development, highly expressed miRNAs may play oncogenic roles by regulating tumour suppressor genes (15), whereas downregulated miRNAs may perform tumour-suppressing functions by directly targeting oncogenes (16).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-379 inhibits cell proliferation and invasion in glioma via targeting metadherin and regulating PTEN/AKT pathway

Zhang

2017

Mol Med Report

View full text Add to dashboard Cite

Numerous microRNAs (miRNAs) are aberrantly expressed in glioma, and implicated in glioma occurrence and development. Therefore, the development of miRNAs as potential therapeutic targets for the treatment of patients with glioma has been proposed. miR‑379 has been shown to be aberrantly expressed in the progression of malignant tumours. However, the expression, biological functions and mechanism of miR‑379 in glioma are yet to be fully understood. Hence, the present study aimed to detect miR‑379 expression, investigate its functional relevance and explore its associated molecular mechanism in glioma. In this study, miR‑379 expression was significantly downregulated in glioma tissues and cell lines. Enforced miR‑379 expression markedly suppressed the cell proliferation and invasion of glioma. Metadherin (MTDH) was identified as a direct target of miR‑379 in glioma. The miR‑379 expression and MTDH mRNA levels exhibited an inverse association in glioma tissues. The restoration of the MTDH expression partially rescued the inhibitory effects of miR‑379 overexpression on glioma cell proliferation and invasion, and the upregulation of miR‑379 inhibited the activation of phosphatase and tensin homolog (PTEN)/AKT serine/threonine kinase (AKT) signaling pathway. Overall, these findings demonstrated that miR‑379 may play tumour‑suppressing roles in glioma through downregulation of MTDH and regulation of the PTEN/AKT signaling pathway, suggesting that miR‑379 might be a possible target for the treatment of patients with this malignancy.

show abstract

MicroRNA-384 represses the growth and invasion of non-small-cell lung cancer by targeting astrocyte elevated gene-1/Wnt signaling

Cited by 27 publications

References 44 publications

Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP

Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP

<p>Mechanism of the Regulatory Effect of Overexpression of circMTO1 on Proliferation and Apoptosis of Hepatoma Cells via miR-9-5p/NOX4 Axis</p>

MicroRNA-379 inhibits cell proliferation and invasion in glioma via targeting metadherin and regulating PTEN/AKT pathway

Contact Info

Product

Resources

About