MicroRNA‑633 enhances melanoma cell proliferation and migration by suppressing KAI1

Abstract

The aim of the present study was to determine the impact of microRNA (miRNA/miR)-633 on the biological properties of malignant melanoma cells. Kang-Ai 1 (KAI1), also known as cluster of differentiation 82, is an important transcriptional regulator and tumor suppressor gene present in different types of tumors. miRNAs that potentially bind with KAI1 were predicted via bioinformatics analyses. In total, six putative miRNA regulators of KAI1 were identified in the present analysis, among which miR-633 was upregulated the most in melanoma tissues compared with the control group. The expression levels of miR-633 and KAI1 in melanoma tissues compared with adjacent normal tissues were then assessed. It was found that miR-633 was significantly upregulated in melanoma cells compared with the control group, whereas the expression levels of KAI1 showed the opposite results. miR-633 was predicted to target the 3′-untranslated region of KAI1 using predictive online tools, and results from luciferase reporter assays confirmed the direct regulation of KAI1 promoter activity by miR-633. Furthermore, miR-633 mimics over expression was shown to suppress both mRNA and protein expression of KAI1, while miR-633 inhibition resulted in decreased viability and migrationin melanoma cells in vitro . Taken together, the present study demonstrated, to the best of the authors' knowledge for the first time, that miR-633 exerts an important role in melanoma through targeting KAI1.

Literature-based translation from synthetic lethality screening into therapeutics targets: CD82 is a novel target for KRAS mutation in colon cancer

“…The miR-633 molecule is another possible CD82 target. According to bioinformatics analyses and in vitro studies, miR-633 could target and regulate CD82 expression [38] . Therefore, the inhibition of miR-633 results in decreased cell viability and migration, indicating that it may be a potential target for CD82.…”

Lower expression of KAI1 as a biomarker of poor survival prognosis of melanoma combined with colorectal cancer metastasis

“…15 For example, it can inhibit the invasion and migration of melanoma cells. 16 KAI1 is a well-characterized solid tumor metastasis inhibitor that does not affect the growth of the primary tumor and is a recognized biomarker for predicting its metastatic potential. 17 KAI1 deletion was associated with aggressive tumor behaviors, such as high drug resistance, low differentiation, high recurrence rate, and shortened disease-free survival and overall survival times.…”

MicroRNA as a Diagnostic Tool, Therapeutic Target and Potential Biomarker in Cutaneous Malignant Melanoma Detection—Narrative Review

“…By transfecting cells with inhibitor of miR-633, cell proliferation and migration were suppressed. MiR-633 may serve as a potential candidate for the diagnosis and treatment of human melanoma [ 62 ].…”