MicroRNA‑663b enhances migration and invasion by targeting adenomatous polyposis coli 2 in colorectal carcinoma cells

Xiao, Fan; Chen, Wangbin; Yu, Chao; Zhao, Gang

doi:10.3892/ol.2020.11482

Cited by 8 publications

(9 citation statements)

References 42 publications

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“…These results suggested a possible role for increased MiR-663b in the development of colorectal polyp-associated cancer. In accordance with these findings, prior research observed a significant increase in the expression levels of miR-663b in CRC cells and showed that the overexpressed miR-663b increased CRC cell proliferation, migration, and invasion, and prevented apoptosis (20) . Another study found considerable overexpression of miR-663b in tissue samples and cell lines from colorectal cancers and concluded that miR-663b plays a crucial role in the development of CRC (21) .…”

Section: Discussionsupporting

confidence: 80%

Role of MicroRNA-663b as an Oncogene in Colorectal Carcinoma on top of familial adenomatous polyposis cells.

T.mohamed

Hafez

A.Idris

et al. 2022

Benha Medical Journal

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Background: Familial polyposis coli (FPC) is almost always benign lesions presenting by bleeding or discovered accidentally during routine colonoscopy. However, malignant transformation could not be excluded and this necessitates early detection in the metaplasia stage. Aim: Evaluation of the ability of estimated tissue expression levels of microRNA-663b (MiR-663b) to distinguish benign from malignant rectal polyps in patients with FPC. Subjects & Methods: Forty-one patients with FPC were assessed clinically, had colonoscopic biopsies, and provided blood samples for estimation of serum carcinoembryonic antigen (CEA) and cancer antigen 19-9 estimate (CA19-9). Tissue samples were separated into two portions, for pathological analysis and PCR analysis to determine the degree of MiR-663b expression in the tissue. Subjects undergoing colonoscopy for other reasons were subjected to a rectal biopsy.Results: The serum levels of CA 19-9 were considerably greater in MRP patients compared to healthy controls. The estimated tissue level of MiR-663b in three benign specimens was greater than the 4th quartile level of control specimens and in the range between the 1st and 2nd quartile levels of malignant specimens; these specimens were thus classified pre-malignant. A multivariate Regression analysis identified elevated miRNA366b gene expression as an independent risk factor for BRP malignant transformation.Conclusion:MiR-663b tissue expression estimation might be utilized to distinguish benign from malignant colorectal polyps. Whenever MiR-663b levels were greater than control levels but below than the levels diagnostic of malignancy, are able to predict the early malignant transformation of benign polyps prior to their being identified by pathology.

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Section: Discussionsupporting

confidence: 80%

Role of MicroRNA-663b as an Oncogene in Colorectal Carcinoma on top of familial adenomatous polyposis cells.

T.mohamed

Hafez

A.Idris

et al. 2022

Benha Medical Journal

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“…Six miRNAs were co-predicted by all three databases [Figure 4A and 4B]. According to these results, circ_0049447 may act as a sponge for certain miRNAs whose validated functions include potential roles in GC development [19–37] [Table 2]. To further explore the underlying mechanisms, we detected the expression of these predicted miRNAs in MGC-803 cells overexpressed circ_0049447.…”

Section: Resultsmentioning

confidence: 88%

“…Promote invasion Breast cancer [28] miR-663b APC2 Promote proliferation, migration, and invasion Colorectal cancer [29] TNK1 Promote proliferation and stemness [30] ERF Promote proliferation and EMT Bladder cancer [31] SMAD7 Promote proliferation and EMT Nasopharyngeal carcinoma [32] TP73 Promote proliferation, inhibit apoptosis Osteosarcoma [33] miR-671-5p TRIM67 Promote proliferation, migration, and invasion Colon cancer [34] APC Promoted migration and invasion Clear cell renal cell carcinoma [35] CDR1 Promote proliferation, migration, and invasion Glioblastoma [36] miR-431 DAB2IP Promote migration and invasion Pancreatic neuroendocrine cancer by inhibiting mesenchymal phenotypes and facilitating epithelial phenotypes. After transfection with the circ_0049447 overexpression plasmid, the mesenchymal markers N-cadherin, twist, vimentin, and b-catenin were downregulated, whereas E-cadherin was upregulated, suggesting that circ_0049447 regulates GC cell migration and invasion through modulating the EMT pathway.…”

Section: Inpp5jmentioning

confidence: 99%

Circ_0049447 acts as a tumor suppressor in gastric cancer through reducing proliferation, migration, invasion, and epithelial-mesenchymal transition

Tang

Guo

et al. 2021

Chinese Medical Journal

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Background: Although increasing abnormal expression of circular RNAs (circRNAs) has been revealed in various cancers, there were a small number of studies about circRNAs in gastric cancer (GC). Here, we explored the expression and function of a novel circRNA, circ_0049447, in GC. Methods: A total of 80 GC tissues and non-tumorous tissues were collected from the First Affiliated Hospital of China Medical University. And all cells were cultured with 10% fetal bovine serum and incubated at 37°C and 5% CO 2 . The expression of circ_0049447 was quantified by real-time polymerase chain reaction. The biological function of circ_0049447 on proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) was evaluated by cell counting kit-8 (CCK-8), colony formation assay, transwell migration and invasion assay, and Western blotting. Luciferase report assay was used to verify the direct binding between circ_0049447 and predicted microRNA (miRNA). Furthermore, a xenograft mouse model was used to validate the function of circ_0049447 in vivo . Results: We demonstrated that circ_0049447 was downregulated in GC ( P < 0.001). The area under the receiver operating characteristic curve reached 0.838, while sensitivity was 82.3% and specificity was 77.2%. CCK-8 and colony formation assay showed that overexpression of circ_0049447 could inhibit the proliferation ( P < 0.05). Transwell migration and invasion assay showed upregulated circ_0049447 could impede migration in GC cells ( P < 0.05). In addition, overexpression of circ_0049447 could impede GC cell EMT. Upregulation of miR-324-5p in GC specimens and direct binding between miR-324-5p with circ_0049447 proven by luciferase reporter assay indicated that circ_0049447 may inhibit GC by sponging certain miRNA. Conclusion: Circ_0049447 acts as a tumor suppressor in GC through reducing proliferation, migration, invasion, and EMT, and it is a promising biomarker for diagnosis.

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“…Several studies have shown that APC/APC2 genes expression is controlled by miRNAs. MiR-663b, [56] miR-501-3p, [57] miR-942, [58] miR-494, [59] miR-103a, [10] miR-1827, [10] miR-582-5P, [60] miR-135 a&b, [61] and miR-582 [62] via targeting APC/APC2 and subsequent activation of Wnt/β catenin signaling, act as oncogenic miRNAs in CRC (Figure 2).…”

Section: Wnt Signaling Intracellular Componentsmentioning

confidence: 99%

MiRNA‐Wnt signaling regulatory network in colorectal cancer

Jafarzadeh

Soltani

2021

J Biochem & Molecular Tox

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Colorectal cancer (CRC) is one of the common malignancies worldwide and the Wnt signaling pathway is recognized as the main disrupted pathway in this malignancy.MicroRNAs (miRNAs) are recognized to contribute to the pathogenesis of CRC by triggering or impeding the Wnt signaling pathway. In addition, transcriptional regulation of miRNAs by canonical Wnt signaling also participates in CRC cell progression. In this review, we present comprehensive literature of the existing data on the interaction of miRNAs and Wnt signaling that could be useful in future studies in the field of CRC management.

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MicroRNA‑663b enhances migration and invasion by targeting adenomatous polyposis coli 2 in colorectal carcinoma cells

Cited by 8 publications

References 42 publications

Role of MicroRNA-663b as an Oncogene in Colorectal Carcinoma on top of familial adenomatous polyposis cells.

Role of MicroRNA-663b as an Oncogene in Colorectal Carcinoma on top of familial adenomatous polyposis cells.

Circ_0049447 acts as a tumor suppressor in gastric cancer through reducing proliferation, migration, invasion, and epithelial-mesenchymal transition

MiRNA‐Wnt signaling regulatory network in colorectal cancer

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