MicroRNA and regulation of auxin and cytokinin signalling during post-mowing regeneration of winter wheat (Triticum aestivum L.)

Cui, Guibin; Zhao, Mei; Zhang, Shumeng; Wang, Zhulin; Meng, Meng; Sun, Fengli; Zhang, Chao; Xi, Yajun

doi:10.1016/j.plaphy.2020.08.032

Cited by 16 publications

(6 citation statements)

References 36 publications

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“…Wheat regrowth after mowing involves both leaf repair and active growth. In this process, endogenous contents of IAA, CTKs, and GA changed in the TN and roots of the wheat, similar to previous studies [67,68]. During leaf development, IAA content reaches a maximum at the leaf primordia formation position, and the GA content also increases [69].…”

Section: Discussionsupporting

confidence: 87%

Effect of Mowing on Wheat Growth at Seeding Stage

Li,

Wang,

et al. 2023

IJMS

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Winter wheat is used as forage at the tillering stage in many countries; however, the regrowth pattern of wheat after mowing remains unclear. In this study, the growth patterns of wheat were revealed through cytological and physiological assessments as well as transcriptome sequencing. The results of agronomic traits and paraffin sections showed that the shoot growth rate increased, but root growth was inhibited after mowing. The submicroscopic structure revealed a decrease in heterochromatin in the tillering node cell and a change in mitochondrial shape in the tillering node and secondary root. Analysis of the transcriptome showed the number of differentially expressed genes (DEGs) involved in biological processes, cellular components, and molecular functions; 2492 upregulated DEGs and 1534 downregulated DEGs were identified. The results of the experimental study showed that mowing induced expression of DEGs in the phenylpropanoid biosynthesis pathway and increased the activity of PAL and 4CL. The upregulated DEGs in the starch and sucrose metabolism pathways and related enzyme activity alterations indicated that the sugar degradation rate increased. The DEGs in the nitrogen metabolism pathway biosynthesis of the amino acids, phenylpropanoid biosynthesis metabolism, and in the TCA pathway also changed after mowing. Hormone content and related gene expression was also altered in the tillering and secondary roots after mowing. When jasmonic acid and ethylene were used to treat the wheat after mowing, the regeneration rate increased, whereas abscisic acid inhibited regrowth. This study revealed the wheat growth patterns after mowing, which could lead to a better understanding of the development of dual-purpose wheat.

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Section: Discussionsupporting

confidence: 87%

Effect of Mowing on Wheat Growth at Seeding Stage

Li,

Wang,

et al. 2023

IJMS

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“…Here, we conducted combined analysis of miRNAs and mRNAs related to maize root system drought stress responses, detected correlations between a series of DEMIRs and DEMRs, and constructed a regulatory network map. Many studies have reported miRNA target genes related to plant hormone signal transduction ( Li et al., 2019 ; Cui et al., 2020 ; Singh et al., 2021 ; Lei et al., 2021 ; Yang et al., 2021b ). Most interestingly, we also found that numerous target genes regulated by miRNAs were also enriched in the three pathways: “phenylpropane biosynthesis”, “glutathione metabolism”, and “starch and sucrose metabolism”.…”

Section: Discussionmentioning

confidence: 99%

Integration of mRNA and microRNA analysis reveals the molecular mechanisms underlying drought stress tolerance in maize (Zea mays L.)

Jiao

Ma²,

Wang³

et al. 2022

Front. Plant Sci.

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Drought is among the most serious environmental issue globally, and seriously affects the development, growth, and yield of crops. Maize (Zea mays L.), an important crop and industrial raw material, is planted on a large scale worldwide and drought can lead to large-scale reductions in maize corn production; however, few studies have focused on the maize root system mechanisms underlying drought resistance. In this study, miRNA–mRNA analysis was performed to deeply analyze the molecular mechanisms involved in drought response in the maize root system under drought stress. Furthermore, preliminary investigation of the biological function of miR408a in the maize root system was also conducted. The morphological, physiological, and transcriptomic changes in the maize variety “M8186” at the seedling stage under 12% PEG 6000 drought treatment (0, 7, and 24 h) were analyzed. With prolonged drought stress, seedlings gradually withered, the root system grew significantly, and abscisic acid, brassinolide, lignin, glutathione, and trehalose content in the root system gradually increased. Furthermore, peroxidase activity increased, while gibberellic acid and jasmonic acid gradually decreased. Moreover, 32 differentially expressed miRNAs (DEMIRs), namely, 25 known miRNAs and 7 new miRNAs, and 3,765 differentially expressed mRNAs (DEMRs), were identified in maize root under drought stress by miRNA-seq and mRNA-seq analysis, respectively. Through combined miRNA–mRNA analysis, 16 miRNA–target gene pairs, comprising 9 DEMIRs and 15 DEMRs, were obtained. In addition, four metabolic pathways, namely, “plant hormone signal transduction”, “phenylpropane biosynthesis”, “glutathione metabolism”, and “starch and sucrose metabolism”, were predicted to have important roles in the response of the maize root system to drought. MiRNA and mRNA expression results were verified by real-time quantitative PCR. Finally, miR408a was selected for functional analysis and demonstrated to be a negative regulator of drought response, mainly through regulation of reactive oxygen species accumulation in the maize root system. This study helps to elaborate the regulatory response mechanisms of the maize root system under drought stress and predicts the biological functions of candidate miRNAs and mRNAs, providing strategies for subsequent mining for, and biological breeding to select for, drought-responsive genes in the maize root system.

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“…miRNAs are a class of non-coding single-stranded small RNAs, which mediate multiple biological processes, including plant growth, development, and stress response by post-transcriptional or translational regulation of target genes ( Kumar et al, 2017 ; Tang and Chu, 2017 ; Nair et al, 2019 ; Cui et al, 2020 ). As reported previously, miRNAs can improve salt tolerance in rice ( Shen et al, 2017 ), barley ( Kuang et al, 2019 ), Arabidopsis thaliana ( Schommer et al, 2014 ), pearl chestnut ( Harshraj et al, 2020 ), and other plants by regulating plant development and stress response.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing-Based Identification of miRNAs and Their Target mRNAs in Wheat Variety Qing Mai 6 Under Salt Stress Condition

Han

Yin

et al. 2021

Front. Genet.

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Soil salinization is one of the major abiotic stresses that adversely affect the yield and quality of crops such as wheat, a leading cereal crop worldwide. Excavating the salt-tolerant genes and exploring the salt tolerance mechanism can help breeding salt-tolerant wheat varieties. Thus, it is essential to identify salt-tolerant wheat germplasm resources. In this study, we carried out a salt stress experiment using Qing Mai 6 (QM6), a salt-tolerant wheat variety, and sequenced the miRNAs and mRNAs. The differentially expressed miRNAs and mRNAs in salt stress conditions were compared with the control. As results, a total of eight salt-tolerance-related miRNAs and their corresponding 11 target mRNAs were identified. Further analysis revealed that QM6 enhances salt tolerance through increasing the expression level of genes related to stress resistance, antioxidation, nutrient absorption, and lipid metabolism balance, and the expression of these genes was regulated by the identified miRNAs. The resulting data provides a theoretical basis for future research studies on miRNAs and novel genes related to salt tolerance in wheat in order to develop genetically improved salt-tolerant wheat varieties.

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MicroRNA and regulation of auxin and cytokinin signalling during post-mowing regeneration of winter wheat (Triticum aestivum L.)

Cited by 16 publications

References 36 publications

Effect of Mowing on Wheat Growth at Seeding Stage

Effect of Mowing on Wheat Growth at Seeding Stage

Integration of mRNA and microRNA analysis reveals the molecular mechanisms underlying drought stress tolerance in maize (Zea mays L.)

High-Throughput Sequencing-Based Identification of miRNAs and Their Target mRNAs in Wheat Variety Qing Mai 6 Under Salt Stress Condition

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