MicroRNAs: Fine Tuners of Monocyte Heterogeneity

Duroux-Richard, Isabelle; Maskey, Robin; Peillex, Cindy; Apparailly, Florence

doi:10.3389/fimmu.2019.02145

Cited by 24 publications

(21 citation statements)

References 69 publications

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“…Circulating monocytes are a heterogeneous population that are classified into three subsets according to CD14 and CD16 expression (classical CD14 ++ CD16 − , intermediate CD14 ++ CD16 + , and nonclassical CD14 + CD16 ++ ). The subsets have characteristic phenotypic, functional, transcriptomic, and epigenetic profiles [ 40 , 41 ], including subset-specific miRNA expression [ 42 ] and DNA methylation profiles linked to distinct immunological processes [ 43 ]. Several studies have shown an increase in circulating CD16 + subsets in elderly vs. young adult monocytes [ 14 , 15 , 17 ], though others have shown no difference in proportions of subsets between elderly and young adults [ 11 ].…”

Section: Resultsmentioning

confidence: 99%

Age-related differences in monocyte DNA methylation and immune function in healthy Kenyan adults and children

et al. 2021

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Background Age-related changes in adaptive and innate immune cells have been associated with a decline in effective immunity and chronic, low-grade inflammation. Epigenetic, transcriptional, and functional changes in monocytes occur with aging, though most studies to date have focused on differences between young adults and the elderly in populations with European ancestry; few data exist regarding changes that occur in circulating monocytes during the first few decades of life or in African populations. We analyzed DNA methylation profiles, cytokine production, and inflammatory gene expression profiles in monocytes from young adults and children from western Kenya. Results We identified several hypo- and hyper-methylated CpG sites in monocytes from Kenyan young adults vs. children that replicated findings in the current literature of differential DNA methylation in monocytes from elderly persons vs. young adults across diverse populations. Differentially methylated CpG sites were also noted in gene regions important to inflammation and innate immune responses. Monocytes from Kenyan young adults vs. children displayed increased production of IL-8, IL-10, and IL-12p70 in response to TLR4 and TLR2/1 stimulation as well as distinct inflammatory gene expression profiles. Conclusions These findings complement previous reports of age-related methylation changes in isolated monocytes and provide novel insights into the role of age-associated changes in innate immune functions.

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Section: Resultsmentioning

confidence: 99%

Age-related differences in monocyte DNA methylation and immune function in healthy Kenyan adults and children

et al. 2021

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“…Targets of differentially expressed miRNAs were mostly related to motility and cell death processes [ 41 ]. miRNA-17, miRNA-18a/b, miRNA-19a/b, miRNA-20b, miR-27a, miRNA-106a, miR-119-5b and miR-345 were overexpressed in classical monocytes, whereas miRNA-132, miRNA-146a, miRNA-342-3p, miR-379, miR-382, miR-411, miR-637 and miR-654-3p were overexpressed in non-classical monocytes [ 50 , 82 ] ( Figure 2 ). Recently, the Ziegler-Heitbrock group presented a lower expression of miR-20a and miR-106b in non-classical monocytes defined by both CD14/CD16 expression and via Slan [ 83 ].…”

Section: Monocytes—diversity and Cancermentioning

confidence: 99%

“… A dot plot representing three subsets of human monocytes according to the expression of CD14 and CD16 supplemented with data concerning upregulated miRNA [ 41 , 49 , 50 ]. …”

Section: Figurementioning

confidence: 99%

The Role of miRNA in Regulating the Fate of Monocytes in Health and Cancer

et al. 2022

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Monocytes represent a heterogeneous population of blood cells that provide a link between innate and adaptive immunity. The unique potential of monocytes as both precursors (e.g., of macrophages) and effector cells (as phagocytes or cytotoxic cells) makes them an interesting research and therapeutic target. At the site of a tumor, monocytes/macrophages constitute a major population of infiltrating leukocytes and, depending on the type of tumor, may play a dual role as either a bad or good indicator for cancer recovery. The functional activity of monocytes and macrophages derived from them is tightly regulated at the transcriptional and post-transcriptional level. This review summarizes the current understanding of the role of small regulatory miRNA in monocyte formation, maturation and function in health and cancer development. Additionally, signatures of miRNA-based monocyte subsets and the influence of exogenous miRNA generated in the tumor environment on the function of monocytes are discussed.

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“…Richard et al focused on the sequencing of miRNAs among monocyte subsets in humans and mice to identify their role in monocyte heterogeneity. From their work, they suggested three miRNAs-miR-21, miR-150, and miR-146aas immune regulators that mediate resolution of inflammation in the myeloid cells (48). MicroRNA profiling of intermediate monocytes (CD14++ CD16+) yielded a unique miRNA profile, and their connected pathways are involved in gene regulation, TLR, and cytokine-mediated signaling, phagocytosis, antigen processing, and presentation, as well as lipid and triglyceride metabolism (49).…”

Section: Monocyte and Macrophage Mirnasmentioning

confidence: 99%

Monocyte and Macrophage miRNA: Potent Biomarker and Target for Host-Directed Therapy for Tuberculosis

et al. 2021

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The end TB strategy reinforces the essentiality of readily accessible biomarkers for early tuberculosis diagnosis. Exploration of microRNA (miRNA) and pathway analysis opens an avenue for the discovery of possible therapeutic targets. miRNA is a small, non-coding oligonucleotide characterized by the mechanism of gene regulation, transcription, and immunomodulation. Studies on miRNA define their importance as an immune marker for active disease progression and as an immunomodulator for innate mechanisms, such as apoptosis and autophagy. Monocyte research is highly advancing toward TB pathogenesis and biomarker efficiency because of its innate and adaptive response connectivity. The combination of monocytes/macrophages and their relative miRNA expression furnish newer insight on the unresolved mechanism for Mycobacterium survival, exploitation of host defense, latent infection, and disease resistance. This review deals with miRNA from monocytes, their relative expression in different disease stages of TB, multiple gene regulating mechanisms in shaping immunity against tuberculosis, and their functionality as biomarker and host-mediated therapeutics. Future collaborative efforts involving multidisciplinary approach in various ethnic population with multiple factors (age, gender, mycobacterial strain, disease stage, other chronic lung infections, and inflammatory disease criteria) on these short miRNAs from body fluids and cells could predict the valuable miRNA biosignature network as a potent tool for biomarkers and host-directed therapy.

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MicroRNAs: Fine Tuners of Monocyte Heterogeneity

Cited by 24 publications

References 69 publications

Age-related differences in monocyte DNA methylation and immune function in healthy Kenyan adults and children

Age-related differences in monocyte DNA methylation and immune function in healthy Kenyan adults and children

The Role of miRNA in Regulating the Fate of Monocytes in Health and Cancer

Monocyte and Macrophage miRNA: Potent Biomarker and Target for Host-Directed Therapy for Tuberculosis

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