MicroRNAs: Harbingers and shapers of periodontal inflammation

Zhou, Xiaofeng; Fallah, Pooria; Pandya, Mirali; Lyu, Huling; Foyle, Deborah; Burch, Dan; Diekwisch, Thomas G. H.

doi:10.1016/j.semcdb.2021.05.030

Cited by 21 publications

(16 citation statements)

References 188 publications

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“…Recent studies have suggested that the progression of both diseases is affected by a wide variety of genes and miRNAs 10 . Although an increasing number of NGS studies have focused on peri‐implantitis and periodontitis, there is a lack of studies in the miRNA field that directly compare these two diseases 21 . Therefore, our study aimed to reveal the differences in miRNA expression profiles, possible signaling pathways, and molecular mechanisms of peri‐implantitis and periodontitis.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA sequence and function analysis in peri‐implantitis and periodontitis: An animal study

Zhang

Yuan

Xue

et al. 2022

J of Periodontal Research

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Objective: To compare miRNA expression levels and predict relevant target genes and signaling pathways in peri-implantitis and periodontitis.Background: There are many differences between periodontitis and peri-implantitis.An understanding of the similarities and differences in the transcriptional patterns of these diseases, as well as the molecular mechanisms, is beneficial for the development of management strategies. Materials and methods:Rat models of periodontitis (PD, n = 6) and peri-implantitis (PI, n = 5) were established by ligation. Implantation without ligation (PIC, n = 5) and normal rats (PDC, n = 6) were used as controls. Micro-CT was used to confirm the successful establishment of the model. Gingiva was harvested for miRNA transcriptome sequencing, and the results were confirmed by qRT-PCR. miRNA target genes were predicted with miRTarBase. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed.Results: Sixty-nine miRNAs were differentially expressed in PI vs. PD, 105 were differentially expressed in PI vs. PIC, and 70 were differentially expressed in PD vs. PDC (log 2 FC ≥1 and padj <0.05). The upregulated genes in all three comparisons were mostly involved in the biological process response to stimulus, whereas most of the downregulated genes were involved in nervous system development (p < .01).The upregulated genes in PI vs. PD and PI vs. PIC were involved in Toll-like receptor signaling and RIG-I-like signaling. The upregulated genes in PI vs. PD were involved in T-and B-cell receptor signaling, apoptosis, and osteoclast differentiation. Focal adhesion was downregulated in all three comparisons, and adherens junction was downregulated in PI vs. PD and PD vs. PDC (p < .1). Conclusion:This study showed differences in the miRNA expression profiles between peri-implantitis and periodontitis and annotated the possible target genes and molecular mechanisms; this study could lay a foundation for the development of management strategies.

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Section: Discussionmentioning

confidence: 99%

MicroRNA sequence and function analysis in peri‐implantitis and periodontitis: An animal study

Zhang

Yuan

Xue

et al. 2022

J of Periodontal Research

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“…Aberrant expression of miRNAs has been observed to dysregulate innate and adaptive immune system cellular responses, leading to the development of periodontitis [20]. MiR-126 is considered as one of the transcriptional regulators of the periodontitis-related mediators [21][22][23]. In various models of in ammation, upregulation of miR-126 has been shown to be bene cial in reducing the recruitment of macrophages and inhibiting in ammation [24,25].…”

Section: Introductionmentioning

confidence: 99%

Genetically Engineered CXCR4-modified Exosomes for Delivery of miR-126 mimics to Macrophages Alleviate Periodontitis

Luo

Chen

et al. 2023

Preprint

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Biofilm related diseases are a group of diseases that tolerant antimicrobial chemotherapies therefore refractory. Periodontitis as a non-device chronic biofilm disease induced by dental plaque, can serve as an excellent in vivo model for studying the important implications of host factors in the biofilm microenvironment. Macrophage activity is one of the key factors that modulate the progression of inflammatory destruction in periodontitis, thus an important host immunomodulatory factor. In this study, the decrease of microRNA-126 (miR-126) with the recruitment of macrophages in periodontitis is confirmed in the clinical samples, and a strategy to target-deliver miR-126 to macrophages is explored.Exosomes overexpressing C-X-C motif chemokine receptor 4 (CXCR4) loaded with miR-126 (CXCR4-miR126-Exo) are successfully constructed, which reduce the off-target delivery to macrophages and regulate macrophages toward the anti-inflammatory phenotype. In vivo local injection ofCXCR4-miR126-Exo into sites of periodontitis in rats effectively reduces the bone resorption and osteoclastogenesis, and inhibited the progression of periodontitis. These results provide new insights for designing novel targeted delivery system of immunomodulatory factor to treat periodontitis and other biofilm related diseases.

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“…In addition to directly attacking vascular endothelium, oxidative stress contributes to inducing inflammation ( Domingueti et al, 2016 ; Bai et al, 2020 ), which has been confirmed in a variety of cardio- and cerebrovascular diseases, such as atherosclerosis (e.g., ox-LDL), diabetes (e.g., high glucose and glycosylation end products), and hypertension (angiotensin II [Ang II]) ( Pleskovič et al, 2017 ; Solis et al, 2021 ). Further research has shown that the mechanism of inflammatory response involves a variety of microRNAs (miR-126, miR-155, miR-221/222, miR-31, miR-17-3p, miR-10a, miR-663, miR-125a-5p, and miR-125b-5p) by regulating downstream target proteins (such as VCAM-1, RGS16, Ets-1, AT1R, E-selectin, ICAM-1, MAP3K7, and βTRC) ( Forouzanfar and Asgharzade, 2020 ; Wu et al, 2020 ; Luan et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Research progress in endothelial cell injury and repair

Huang

Song

et al. 2022

Front. Pharmacol.

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Endothelial cells, which are important metabolic and endocrine cells, play an important role in regulating vascular function. The occurrence and development of various cardiovascular and cerebrovascular diseases are associated with endothelial dysfunction. However, the underlying mechanism of vascular endothelial injury is not fully understood. It has been reported that the mechanism of endothelial injury mainly involves inflammation and oxidative stress. Moreover, endothelial progenitor cells are regarded as important contributors in repairing damaged endothelium. Multiple interventions (including chemical drugs and traditional Chinese medicines) exert endothelial protection by decreasing the release of inducing factors, suppressing inflammation and oxidative stress, and preventing endothelial cell senescence.

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MicroRNAs: Harbingers and shapers of periodontal inflammation

Cited by 21 publications

References 188 publications

MicroRNA sequence and function analysis in peri‐implantitis and periodontitis: An animal study

MicroRNA sequence and function analysis in peri‐implantitis and periodontitis: An animal study

Genetically Engineered CXCR4-modified Exosomes for Delivery of miR-126 mimics to Macrophages Alleviate Periodontitis

Research progress in endothelial cell injury and repair

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